期刊
METHODS
卷 118, 期 -, 页码 146-162出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymeth.2016.12.002
关键词
Analytical ultracentrifugation; Computational modeling; Disordered and flexible systems; Dynamic light scattering; Size exclusion chromatography; Size exclusion chromatography coupled to multi-angle laser light scattering; Small angle neutron scattering; Small angle X-ray scattering
资金
- University of Lethbridge Start-up fund
- Polish National Science Centre [2012/04/A/NZ2/00455]
- European Research Council [RNA+P=123D]
- Foundation for Polish Science (FNP)
- Natural Sciences and Engineering Research Council of Canada [RGPIN-2015-06142]
- Cancer Research Society
The diverse functional cellular roles played by ribonucleic acids (RNA) have emphasized the need to develop rapid and accurate methodologies to elucidate the relationship between the structure and function of RNA. Structural biology tools such as X-ray crystallography and Nuclear Magnetic Resonance are highly useful methods to obtain atomic-level resolution models of macromolecules. However, both methods have sample, time, and technical limitations that prevent their application to a number of macromolecules of interest. An emerging alternative to high-resolution structural techniques is to employ a hybrid approach that combines low-resolution shape information about macromolecules and their complexes from experimental hydrodynamic (e.g. analytical ultracentrifugation) and solution scattering measurements (e.g., solution X-ray or neutron scattering), with computational modeling to obtain atomic level models. While promising, scattering methods rely on aggregation-free, monodispersed preparations and therefore the careful development of a quality control pipeline is fundamental to an unbiased and reliable structural determination. This review article describes hydrodynamic techniques that are highly valuable for homogeneity studies, scattering techniques useful to study the low-resolution shape, and strategies for computational modeling to obtain high-resolution 3D structural models of RNAs, proteins, and RNA-protein complexes. (C) 2016 The Author(s). Published by Elsevier Inc.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据