期刊
ECOTOXICOLOGY
卷 24, 期 10, 页码 2200-2206出版社
SPRINGER
DOI: 10.1007/s10646-015-1553-2
关键词
Mercury; Bioavailability; Humic acids; EDTA; Bioreporter; Escherichia coli ARL1
资金
- Natural Science Foundation of China [41101294]
- Natural Science Foundation of Jiangsu Province, China [BK2010572]
- Jiangsu Government Scholarship for Overseas Studies
- Strategic Priority Research Program of the Chinese Academy of Sciences [XDB14020204]
- University of Tennessee's Center for Environmental Biotechnology
- Institute for a Secure and Sustainable Environment
A luxCDABE-based genetically engineered bacterial bioreporter (Escherichia coli ARL1) was used to detect bioavailable ionic mercury (Hg(II)) and investigate the effects of humic acids and ethylenediaminetetraacetic acid (EDTA) on the bioavailability of mercury in E. c oli. Results showed that the E. c oli ARL1 bioreporter was sensitive to mercury, with a detection limit of Hg(II) of 0.5 A mu g/L and a linear dose/response relationship up to 2000 A mu g Hg(II)/L. Humic acids and EDTA decreased the Hg(II)-induced bioluminescent response of strain ARL1, suggesting that the two organic ligands reduced the bioavailability of Hg(II) via complexation with Hg(II). Compared with traditional chemical methods, the use of E. c oli ARL1 is a cost-effective, rapid, and reliable approach for measuring aqueous mercury at very low concentrations and thus has potential for applications in field in situ monitoring.
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