Article
Materials Science, Biomaterials
Cynthia M. Co, Samira Izuagbe, Jun Zhou, Ning Zhou, Xiankai Sun, Joseph Borrelli, Liping Tang
Summary: A novel cell-based therapy has been developed for treating articular cartilage injuries, involving the pre-targeting of apoptotic chondrocytes and the delivery of healthy, metabolically active chondrocytes using click chemistry. The effectiveness of this approach was demonstrated in reducing cartilage degeneration and enhancing ECM regeneration in an ex vivo human cartilage explant PTOA model. This Tz-TCO click chemistry-mediated cell delivery approach shows great potential in clinical applications for targeting and treating cartilage injury.
REGENERATIVE BIOMATERIALS
(2021)
Article
Materials Science, Multidisciplinary
Rong Jin, Minqiang Wang, Liefu Zhou, Zhengshi Yang, Qing Chang, Aoneng Cao
Summary: Polymeric nanogels from clickable dextran prodrugs were designed for pH-responsive drug release against solid tumors. The nanogels showed slow DOX release under physiological conditions and faster release in an acidic environment. In vitro and in vivo studies demonstrated significant antitumor activity and improved drug accumulation for the nanogels compared to free DOX, highlighting their potential for cancer chemotherapy.
ACS APPLIED POLYMER MATERIALS
(2021)
Article
Chemistry, Multidisciplinary
Shanmugam Revathi, Aditya L. Shinde, Mulimani K. Rajashekhar, Debasish Mandal, Amit Ranjan Maity, Somenath Garai, Tapas Ghatak
Summary: A novel synthetic approach using innovative metal-free N-heterocyclic imino catalysts has been developed to efficiently synthesize regioselective 1,4-disubstituted 1,2,3-triazoles. Experimental observations have been supported by TD-DFT computational studies. This state-of-the-art metal-free approach serves as an alternative to CuAAC and shows promising potential in the field of synthesizing regioselective 1,4-triazoles.
CHEMICAL COMMUNICATIONS
(2023)
Review
Chemistry, Multidisciplinary
Hong Yeol Yoon, Donghyun Lee, Dong-Kwon Lim, Heebeom Koo, Kwangmeyung Kim
Summary: Traditional organic chemical reactions often require organic solvents, toxic catalysts, heat, or high pressure. However, copper-free click chemistry offers a more favorable alternative with its fast reaction rates and orthogonality in water, buffer solutions, and physiological conditions without the need for toxic catalysts. This has led to its growing interest in biomedical research, where it has been used in various applications such as molecular imaging and therapy.
ADVANCED MATERIALS
(2022)
Article
Nanoscience & Nanotechnology
Hua-Yun Lee, Po-Yu Chiang, Hsi-Ju Wu, Ting-Yi Wang, Tzu-Ching Yang, Wei-Chieh Cheng, Lee-Chiang Lo, Wei-Ssu Liao
Summary: A bottom-up approach was adopted to synthesize azido-functionalized carbon dots (CDs) and functionalize them with other molecules using click chemistry. Folate-decorated CDs showed fluorescence in tumor cells with elevated folate receptors. This method has great potential for future applications.
ACS APPLIED NANO MATERIALS
(2022)
Article
Materials Science, Biomaterials
Wenjie Han, Miao He, Yunhan Zhang, Junxiang Zhou, Zhigang Li, Xiaoyu Liu, Xiaoyun Sun, Xue Yin, Dongbao Yao, Haojun Liang
Summary: The emergence of synthetic biology enables the construction of cell-assembly biosystems with specific gene expression and function. This study explores the process of cell migration and assembly regulated by cadherins by constructing different cadherin plasmids and introducing them into different cells. The findings have potential applications in the construction of self-assembling synthetic tissues and organoids.
JOURNAL OF MATERIALS CHEMISTRY B
(2022)
Article
Biochemical Research Methods
Ariel Helms Thames, Sam J. Moons, Derek A. Wong, Thomas J. Boltje, Bruce S. Bochner, Michael C. Jewett
Summary: The study developed a cell-free glycoprotein synthesis platform called GlycoCAP for building noncanonical glycans. Through optimization, the synthesis achieved over 60% sialylation efficiency with a noncanonical azido-sialic acid. The study also demonstrated the conjugation of a model fluorophore using click chemistry. GlycoCAP is expected to facilitate the development and discovery of glycan-based drugs.
ACS SYNTHETIC BIOLOGY
(2023)
Article
Biochemical Research Methods
Zhuhao Wu, Bin Chen, Yue Wu, Yu Xia, Hui Chen, Zhiyi Gong, Hang Hu, Zhao Ding, Shishang Guo
Summary: A scaffold-free acoustofluidic device was developed to fabricate heterotypic cell spheroids with complexity over cell architectures and components, demonstrating potential in various areas such as tumor research, tissue engineering, developmental biology, and drug discovery.
Article
Pharmacology & Pharmacy
Peter Bitsch, Eva S. Baum, Irati Beltran Hernandez, Sebastian Bitsch, Jakob Harwood, Sabrina Oliveira, Harald Kolmar
Summary: In this study, nanobody dextran polymer conjugates (dextraknobs) loaded with small molecules were developed for cancer diagnostics and therapy. It was found that using smaller sized dextrans improved the homogeneous penetration of the conjugates into tumor spheroids. Additionally, the dextraknobs showed potent cell killing effects in photodynamic therapy. The monovalent nanobody conjugates exhibited deeper and more homogeneous penetration compared to the bivalent conjugates. These findings encourage further development of dextraknobs.
Review
Chemistry, Analytical
Shinsuke Segawa, Xuewen He, Ben Zhong Tang
Summary: The emergence of 'click chemistry' and 'aggregation-induced emission (AIE)' as transformative research paradigms has had significant impacts on academia in the early 21st century. Click chemistry has simplified complex molecular syntheses and functionalization, while AIE has offered profound insight into solid-state photophysics and promoted the creation of aggregate materials. This review showcases the synergetic applications of AIE probes and metal-free click or bioorthogonal reactions, highlighting both the achievements and unexplored avenues in this promising field.
Review
Chemistry, Multidisciplinary
Vincent Rigolot, Christophe Biot, Cedric Lion
Summary: The field of bioorthogonal chemistry has made significant advancements in chemical biology, allowing researchers to probe biomolecules in complex biological environments. However, implementing bioorthogonal ligations within cells remains a challenging task that requires careful planning and overcoming various limiting factors.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Chemistry, Organic
Debabrata Mondal, Sohom Kundu, Emad Elramadi, Isa Valiyev, Michael Schmittel
Summary: We demonstrated the self-healing of a molecular machine's shuttling dynamics through negative feedback. Addition of zinc(II) to the copper(I)-loaded [2]rotaxane shuttle [Cu(R)](+) led to the replacement of copper(I) with static zinc(II)-loaded [2]rotaxane [Zn(R)](2+). Loss of dynamics was accompanied by fluorescence enhancement at lambda = 364 nm. However, the released copper(I) ions catalyzed the formation of a bis-triazole ligand, which selectively captured zinc(II), resulting in the restoration of copper(I) in the rotaxane and the regained dynamic shuttling motion of [Cu(R)](+). The healing process was conveniently monitored through diagnostic fluorescence changes.
Article
Chemistry, Multidisciplinary
Leona L. Rodrigues, Aaron S. Micallef, Michael C. Pfrunder, Vinh X. Truong, John C. McMurtrie, Tim R. Dargaville, Anja S. Goldmann, Florian Feist, Christopher Barner-Kowollik
Summary: A highly efficient ligation system based on visible light-induced rearrangement was introduced, generating a thiophenol for rapid thiol-Michael additions without the need for additives. The self-catalyzed ligation through pyridine mediated deprotonation of the photochemically generated thiophenol was described, leading to additive-free thiol-Michael reactions. The study explored various reaction conditions, solvents, and substrates, showcasing excellent end group fidelity in polymer modification.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Polymer Science
Le Chang, Cong Wang, Shuang Han, Xiaolong Sun, Feng Xu
Summary: This article presents a smart hydrogel system that can undergo structural transformations under neutral aqueous conditions, regulating stiffness and properties through dynamic covalent bonding. With good biocompatibility, this system shows great potential in biomedical applications such as tissue engineering and cell therapy.
Article
Biochemical Research Methods
Alexandra Bristiel, Manon Cadinot, Mathieu Pizzonero, Frederic Taran, Dominique Urban, Raphael Guignard, Dominique Guianvarc'h
Summary: The researchers developed a facile method for conjugating relevant probes, ligands, or delivery agents onto oligonucleotides (ONs), which is important for both fundamental studies in chemical biology and improving the pharmacology of ONs in medicinal chemistry. They introduced novel 2'-functionalized nucleoside phosphoramidite monomers that contain bioorthogonal methylcyclopropene or sydnone moieties for the first time to ON solid-phase synthesis, allowing traceless ON postsynthetic modifications with reactive complementary probes through inverse electron-demand Diels-Alder reactions or strain-promoted sydnone-alkyne cycloaddition. These findings expand the bioorthogonal phosphoramidite building blocks and provide a new method for generating labeled ONs.
BIOCONJUGATE CHEMISTRY
(2023)
Article
Materials Science, Biomaterials
Mohammadreza Kasravi, Alireza Yaghoobi, Tahereh Tayebi, Mahsa Hojabri, Abdolkarim Talebi Taheri, Fatemeh Shirzad, Bahram Jambar Nooshin, Radman Mazloomnejad, Armin Ahmadi, Fatemeh A. Tehrani, Ghasem Yazdanpanah, Mohammad Hadi Farjoo, Hassan Niknejad
Summary: As a promising approach in translational medicine, the decellularization of discarded livers to produce bioscaffolds that support recellularization has potential in overcoming the limitations of conventional liver transplantation. In this study, the researchers investigated the use of matrix metalloproteinase (MMP) inhibition to preserve the extracellular matrix (ECM) during liver decellularization. The results demonstrated that the application of an MMP inhibitor significantly improved the preservation of ECM components and mechanical properties of the bioscaffolds, which supported cell viability and function in vitro. The study also confirmed that the MMP inhibition led to the inhibition of MMP2 and MMP9, providing a novel method to enhance ECM preservation during liver decellularization.
BIOMATERIALS ADVANCES
(2024)
Article
Materials Science, Biomaterials
Mohammadsadegh Nadimifar, Weiguang Jin, Clara Coll-Satue, Gizem Bor, Paul Joseph Kempen, Ali Akbar Moosavi-Movahedi, Leticia Hosta-Rigau
Summary: This study presents a metal-phenolic self-assembly approach that can prepare nanoparticles fully made of hemoglobin. The nanoparticles exhibit good oxygen binding and releasing capabilities.
BIOMATERIALS ADVANCES
(2024)
Article
Materials Science, Biomaterials
Jyoti Kumari, Roel Hammink, Jochem Baaij, Frank A. D. T. G. Wagener, Paul H. J. Kouwer
Summary: Fibrosis is the formation of fibrous connective tissue in response to injury, leading to organ dysfunction. A novel hybrid hydrogel combining synthetic polyisocyanide with hyaluronic acid has been developed, showing strong antifibrotic properties.
BIOMATERIALS ADVANCES
(2024)
Letter
Materials Science, Biomaterials
Melissa Machado Rodrigues, Cristian Padilha Fontoura, Charlene Silvestrin Celi Garcia, Sandro Tomaz Martins, Joao Antonio Pegas Henriques, Carlos Alejandro Figueroa, Mariana Roesch Ely, Cesar Aguzzoli
BIOMATERIALS ADVANCES
(2024)
Article
Materials Science, Biomaterials
Jessica Polak, David Sachs, Nino Scherrer, Adrian Suess, Huan Liu, Mitchell Levesque, Sabine Werner, Edoardo Mazza, Gaetana Restivo, Mirko Meboldt, Costanza Giampietro
Summary: Human skin equivalents (HSEs) play a crucial role in tissue engineering. This study introduces a 3D-printed culture insert to apply a static radial constraint on HSEs and examines its effects on tissue characteristics. The results show that the diameter of the culture insert significantly influences tissue contraction, fibroblast and matrix organization, keratinocyte differentiation, epidermal stratification, and basement membrane formation. This study provides important insights for the design of skin tissue engineering.
BIOMATERIALS ADVANCES
(2024)
Review
Materials Science, Biomaterials
Shiliang Chen, Tianming Du, Hanbing Zhang, Jing Qi, Yanping Zhang, Yongliang Mu, Aike Qiao
Summary: This paper reviewed the primary methods for improving the overall properties of biodegradable zinc stents. It discussed the mechanical properties, degradation behavior, and biocompatibility of various improvement strategies. Alloying was found to be the most common, simple, and effective method for improving mechanical properties. Deformation processing and surface modification further improved the mechanical properties and biological activity of zinc alloys. Meanwhile, structural design could endow stents with special properties. Manufacturing zinc alloys with excellent properties and exploring their interaction mechanism with the human body are areas for future research.
BIOMATERIALS ADVANCES
(2024)