P(TA) macro-, micro-, nanoparticle-embedded super porous p(HEMA) cryogels as wound dressing material

Super porous poly(2-hydroxy ethyl methacrylate) (p(HEMA)) cryogel was successfully synthesized by using polyethylene glycol diacrylate (p(EGDA)) crosslinker under cryogenic conditions. Poly(Tannic acid) (p(TA)) macro-, micro-, and nanopartides prepared from a natural polyphenol, tannic acid (TA), were embedded into p(HEMA) cryogel networks to obtain composite p(TA) particle-embedded p(HEMA) cryogel. Different size ranges of spherical p(TA) particles, 2000-500 mu m, 500-200 mu m, 200-20 mu m, and 20-0.5 mu m size, were included in the cryogel network and illustrated by digital camera, optic microscope, and SEM images of the microgelcryogel network. The swelling properties and moisture content of p(TA) microgel-embedded p(HEMA) cryogel were investigated at wound healing pH conditions such as pH 5.4, 7A, and 9 at 37.5 degrees C, and the highest swelling capacity was found at pH 9 with 972 +/- 2% swelling in 30 s. Higher amounts of DI water were quickly absorbed by p(HEMA)-based cryogel, and moisture retention within the cryogel structure for a longer time period at room temperature is due to existence of p(TA) particles. Degradation profiles of p(TA) particle-embedded p(HEMA) cryogel were shown to be controlled by different pH conditions, and a linear release profile was found with total cumulative release of 5.8 +/- 0.8 mg/g TA up to 12 days at pH 7A and 37.5 degrees C. The antioxidant behavior of degraded p(TA) particles from p(HEMA) cryogel were found as 46 +/- 1 mu g mL(-1) gallic acid equivalent and 165 +/- 18 mM trolox equivalent g(-1). The p(TA) particle-embedded p(HEMA) cryogel has high hemocompatibility with 0.0158 +/- 0.0126% hemolysis ratio, and effective hemostatic properties with 8.1 +/- 0.9 blood clotting index. (C) 2016 Elsevier B.V. All rights reserved.