Identification of genotoxic compounds in crude oil using fractionation according to distillation, polarity and Kow

We examined the degree of DNA damage caused by fractions of crude oil in accordance with the boiling points, polarity and log Km. Relatively high DNA damage was observed in the aromatic fraction (290-330 degrees C) and resin and polar fraction (350-400 degrees C). The resin and polar fraction showed relatively high genotoxicity compared with the aliphatic and aromatic fraction at the 1-4 log K-ow range. At the 6-7 log K-ow, range, the aromatic fraction showed relatively high DNA damage compared with the aliphatic and resin and polar fraction. In particular, every detailed fraction in accordance with the log K-ow values (aliphatic and aromatic (310-320 degrees C) and resins and polar fractions (370-380 degrees C)) showed one or less than one DNA damage. However, the fractions before separation in accordance with log Kow values (aliphatic and aromatic (310-320 degrees C) and resin and polar (370-380 degrees C) fractions) showed high DNA damage. Thus, we confirm the synergistic action between the detailed compounds. (C) 2016 Elsevier Ltd. All rights reserved.