期刊
MARINE POLLUTION BULLETIN
卷 115, 期 1-2, 页码 39-46出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.marpolbul.2016.11.007
关键词
Biodegradation; Polycyclic aromatic hydrocarbons; Saudi Arabia; Pseudomonas aeruginosa; P. citronellolis; Ochrobactrum intermedium and Cupriavidus taiwanensis
资金
- National Plan for Science, Technology and Innovation (MAARIFA)-King Abdulaziz City for Science and Technology through the Science & Technology Unit at Kihg Fand University of Petroleum & Minerals (KFUPM)-the Kingdom of Saudi Arabia [08-ENV458-4]
Contaminated sediment samples were collected from the Eastern Province, Saudi Arabia for isolation of pyrene-and phenanthrene-degrading bacteria by enrichment method. Four isolates were morphologically characterized as Gram-negative rod strains and 16S rRNA sequence analysis revealed the isolates as closely related to Pseudomonas aeruginosa, P. citronellolis, Ochrobactrum intermedium and Cupriavidus taiwanensis. Degradation of the polycyclic aromatic hydrocarbons (PAHs) by the latter three strains was investigated in liquid cultures. Results of concentration reduction analyzed with gas chromatography show that P. citronellolis_LB was efficient in removing phenanthrene, degrading 94% of 100 ppm in 15 days while 0. intermedium_BC1 was more efficient in pyrene-removal, degrading 62% in 2 weeks. Furthermore, bacterial growth assessment using optical density and population counts revealed the latter as more suitable for microbial growth analysis in PAH-containing cultures. In conclusion, the isolated bacterial strains could be further developed for efficient use in biodegradation of PAH. (C) 2016 Elsevier Ltd. All rights reserved.
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