期刊
MARINE DRUGS
卷 15, 期 11, 页码 -出版社
MDPI AG
DOI: 10.3390/md15110338
关键词
Crassostrea gigas; polysaccharides; aqueous two-phase system; anti-tumor activity
资金
- National Natural Science Foundation of China [21476135]
- Educational Commission of Guangdong Province, China [2016KZDXM014]
- Ocean and Fisheries Administration Project of Guangdong Province, China
- Research and Development Start-Up Foundation of Shantou University [NTF16014]
In this study, a simple aqueous two-phase system (ATPS) was employed for concurrent purification of oyster polysaccharides. The chemical structure and anti-tumor activities of purified oyster polysaccharides (OP-1) were also investigated. Under optimal ATPS conditions, oyster polysaccharides can be partitioned in the bottom phase with 67.02% extraction efficiency. The molecular weight of OP-1 was determined as 3480 Da. OP-1 is a (14)--d-glucosyl backbone and branching points located at O-3 of glucose with a terminal-d-Glcp. The anti-tumor activity assay showed that OP-1 exhibited good activities, including promotion of splenocyte proliferation, IL-2 release, and inhibition of HepG2 cell proliferation. Additionally, OP-1 had no in vivo toxicity. This finding suggests that ATPS is a much simpler and greener system, and it opens up new possibilities in the large-scale separation of active polysaccharides from oysters. OP-1 could be used by the health food and pharmaceutical therapies as potential anti-cancer adjuvants.
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