31P magnetization transfer magnetic resonance spectroscopy: Assessing the activation induced change in cerebral ATP metabolic rates at 3 T

PurposeIn vivo P-31 magnetic resonance spectroscopy (MRS) magnetization transfer (MT) provides a direct measure of neuronal activity at the metabolic level. This work aims to use functional P-31 MRS-MT to investigate the change in cerebral adenosine triphosphate (ATP) metabolic rates in healthy adults upon repeated visual stimuli. MethodsA magnetization saturation transfer sequence with narrowband selective saturation of -ATP was developed for P-31 MT experiments at 3T. ResultsUsing progressive saturation of -ATP, the intrinsic T-1 relaxation times of phosphocreatine (PCr) and inorganic phosphate (Pi) at 3T were measured to be 5.10.8 s and 3.0 +/- 1.4 s, respectively. Using steady-state saturation of -ATP, a significant 24%+/- 14% and 11%+/- 7% increase in the forward creatine kinase (CK) pseudo-first-order reaction rate constant, k(1), was observed upon visual stimulation in the first and second cycles, respectively, of a paradigm consisting of 10-minute rest followed by 10-minute stimulation, with the measured baseline k(1) being 0.35 +/- 0.04 s(-1). No significant changes in forward ATP synthase reaction rate, PCr/-ATP, Pi/-ATP, and nicotinamide adenine dinucleotide/-ATP ratios, or intracellular pH were detected upon stimulation. ConclusionThis work demonstrates the potential of studying cerebral bioenergetics using functional P-31 MRS-MT to determine the change in the forward CK reaction rate at 3T. Magn Reson Med 79:22-30, 2018. (c) 2017 The Authors Magnetic Resonance in Medicine published by Wiley Periodicals, Inc. on behalf of International Society for Magnetic Resonance in Medicine. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.