Article
Multidisciplinary Sciences
Simon Renders, Arthur Flohr Svendsen, Jasper Panten, Nicolas Rama, Maria Maryanovich, Pia Sommerkamp, Luisa Ladel, Anna Rita Redavid, Benjamin Gibert, Seka Lazare, Benjamin Ducarouge, Katharina Schoenberger, Andreas Narr, Manon Tourbez, Bertien Dethmers-Ausema, Erik Zwart, Agnes Hotz-Wagenblatt, Dachuan Zhang, Claudia Korn, Petra Zeisberger, Adriana Przybylla, Markus Sohn, Simon Mendez-Ferrer, Mathias Heikenwaelder, Maik Brune, Daniel Klimmeck, Leonid Bystrykh, Paul S. Frenette, Patrick Mehlen, Gerald de Haan, Nina Cabezas-Wallscheid, Andreas Trumpp
Summary: Haematopoietic stem cells are characterized by their self-renewal potential and dormancy. This study shows that niche-produced netrin-1 preserves HSC quiescence and self-renewal through neogenin-1, with a decline in netrin-1 production during aging leading to decreased Neo1-mediated HSC self-renewal.
NATURE COMMUNICATIONS
(2021)
Review
Cell Biology
Yan Man, Xiangmei Yao, Tonghua Yang, Yajie Wang
Summary: The self-renewal and differentiation of hematopoietic stem cells are tightly controlled by various cells and cytokines in the bone marrow microenvironment. Changes in the niche composition can lead to hematological malignancies, and processes like homing, proliferation, and differentiation of HSCs are crucial for the success of hematopoietic stem cell transplantation. Single-cell sequencing and in vivo imaging are powerful tools for studying the bone marrow microenvironment in diseases like leukemia and after transplantation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Sarah Ennis, Alessandra Conforte, Eimear O'Reilly, Javid Sabour Takanlu, Tatiana Cichocka, Sukhraj Pal Dhami, Pamela Nicholson, Philippe Krebs, Pilib O. Broin, Eva Szegezdi
Summary: In this study, a single-cell gene expression database of 339,381 bone marrow cells was established to comprehensively characterize the microenvironment of both healthy and acute myeloid leukemia (AML). Significant changes in cell type proportions and gene expression were observed in AML, indicating disruption of the entire niche. Predicted interactions between hematopoietic stem and progenitor cells (HSPCs) and other bone marrow cell types were also explored, revealing an expansion of interactions in AML that promote HSPC-cell adhesion, immunosuppression, and cytokine signaling. Transforming growth factor b1 (TGFB1)-related interactions were particularly widespread and were shown to drive AML cell quiescence in vitro. These findings highlight potential mechanisms of enhanced AML-HSPC competitiveness and a skewed microenvironment fostering AML growth.
Article
Cell & Tissue Engineering
Zhengqi Wang, Grace Emmel, Hong Seo Lim, Wandi Zhu, Astrid Kosters, Eliver E. B. Ghosn, Peng Qiu, Kevin D. Bunting
Summary: This study reveals the important role of STAT5ab gene in both hematopoietic and stromal cells, and the deletion of STAT5ab using specific Cre promoters leads to a reduction in multipotent hematopoietic progenitors. Furthermore, STAT5ab is involved in the secretion of niche factors in mesenchymal stem cells and the differentiation priming of hematopoietic stem cells.
Article
Cell & Tissue Engineering
Dachuan Zhang, Xin Gao, Huihui Li, Daniel K. Borger, Qiaozhi Wei, Eva Yang, Chunliang Xu, Sandra Pinho, Paul S. Frenette
Summary: This study reveals that the microbiota regulates hematopoietic stem cells (HSCs) self-renewal and differentiation by modulating local iron availability in the bone marrow. Microbiota depletion enhances HSC self-renewal but compromises differentiation under stress conditions. The interplay between the microbiota, macrophages, and iron is essential for regulating critical HSC fate decisions.
Article
Oncology
Dhrishya Dharmapal, Athira Jyothy, Amrutha Mohan, P. G. Balagopal, Nebu Abraham George, Paul Sebastian, Tessy Thomas Maliekal, Suparna Sengupta
Summary: Recent cancer research has highlighted the importance of cancer stem cell (CSC) niches in tumor progression and treatment outcomes. The role of TUBB4B, a membrane trafficking protein, in sustaining CSCs in oral cancer has been investigated. It was found that TUBB4B may play a crucial role in constituting the CSC niche, potentially through its cooperation with signaling protein Ephrin-B1, which affects the self-renewal of oral cancer stem cells and leads to a poor prognosis.
FRONTIERS IN ONCOLOGY
(2021)
Article
Pathology
Christina H. Stuelten, Nicolas Melis, Bhagawat Subramanian, Yi Tang, Megan Kimicata, John P. Fisher, Roberto Weigert, Ying E. Zhang
Summary: This study found that Smurf2 plays an important role in cutaneous wound healing. Loss of Smurf2 exacerbates early inflammation in the wounds and leads to narrower wounds but with greater breaking strength. In addition, loss of Smurf2 also increases the linearization of collagen bundles in normal and wounded skin.
AMERICAN JOURNAL OF PATHOLOGY
(2022)
Review
Chemistry, Medicinal
Annamaria Aprile, Silvia Sighinolfi, Laura Raggi, Giuliana Ferrari
Summary: Research in the last decade has shown multiple alterations of the BM niche in beta-thalassemia and sickle cell disease, emphasizing the importance of understanding the interaction between HSC biology and the BM microenvironment in improving clinical outcomes of transplantation.
Article
Biology
Jose Gabriel Barcia Duran, Tyler Lu, Sean Houghton, Fuqiang Geng, Ryan Schreiner, Jenny Xiang, Shahin Rafii, David Redmond, Raphael Lis
Summary: Jak3, a member of the Jak family of secondary messengers, is expressed in various endothelial cell types, with high expression in the bone marrow; Jak3 in the bone marrow niche plays a crucial role in maintaining long-term repopulating hematopoietic stem cells; Overexpression of Jak3 in endothelial cells enhances the potential for expanding LT-HSCs.
COMMUNICATIONS BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Peng Zhang, Pinpin Sui, Shi Chen, Ying Guo, Ying Li, Guo Ge, Ganqian Zhu, Hui Yang, Cody M. Rogers, Patrick Sung, Stephen D. Nimer, Mingjiang Xu, Feng-Chun Yang
Summary: INTS11, a catalytic subunit of the INT complex, is essential for maintaining HSPC functions by interacting with the PRC2 complex. Loss of INTS11 destabilizes PRC2, leading to decreased H3K27me3 levels and derepression of PRC2 target genes in HSPCs. Reintroducing INTS11 or PRC2 proteins in Ints11-deficient HSPCs restores PRC2 levels, H3K27me3, and overall HSPC functions.
Article
Medicine, Research & Experimental
Yan Lin, Quan Gu, Shihong Lu, Zengkai Pan, Zining Yang, Yapu Li, Shangda Yang, Yanling Lv, Zhaofeng Zheng, Guohuan Sun, Fanglin Gou, Chang Xu, Xiangnan Zhao, Fengjiao Wang, Chenchen Wang, Shiru Yuan, Xiaobao Xie, Yang Cao, Yue Liu, Weiying Gu, Tao Cheng, Hui Cheng, Xiaoxia Hu
Summary: Severe acute graft-versus-host disease (aGVHD) is a complication of allogeneic hematopoietic stem cell transplantation. The bone marrow (BM) niche is damaged in aGVHD hosts, and the use of the JAK1/2 inhibitor ruxolitinib can restore BM mesenchymal stromal cell (BMSC) function and improve hematopoietic dysfunction caused by aGVHD.
JOURNAL OF CLINICAL INVESTIGATION
(2023)
Article
Cell Biology
Krystle Joy Ng, Jamien Lim, Yolanda Nwayhtetmaw Tan, Delia Quek, Zoe Lim, Nikolaos Pantelireis, Carlos Clavel
Summary: This study reveals that dermal papilla cells within the hair follicle not only induce hair growth but also regulate hair color. Sox2 is identified as a key regulator of melanocyte signaling, affecting the expression of genes related to melanin production through the regulation of Agouti, Corin, and bone morphogenic protein signaling.
Article
Hematology
Yang Liu, Qi Chen, Hyun-Woo Jeong, Dong Han, Joerg Fabian, Hannes C. A. Drexler, Martin Stehling, Hans R. Schoeler, Ralf H. Adams
Summary: This study reveals that dopamine derived from sympathetic nerves directly controls the behavior of hematopoietic stem and progenitor cells through D-2 subfamily dopamine receptors. Dopamine signaling affects proliferation, frequency, and transplantation efficiency of HSPCs.
Review
Biochemistry & Molecular Biology
Nicole Pui-Yu Ho, Hitoshi Takizawa
Summary: This article summarizes the current understanding of how hematopoietic stem cell functions are maintained, damaged, or exhausted during acute, prolonged, and pathological inflammatory conditions. It also highlights the impact of inflammation-altered hematopoietic stem cell niche on escalating the insults on hematopoietic stem cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Hematology
Alice Tang, Ana Nicolle Strat, Mahmudur Rahman, Helen Zhang, Weili Bao, Yunfeng Liu, David Shi, Xiuli An, Deepa Manwani, Patricia Shi, Karina Yazdanbakhsh, Avital Mendelson
Summary: Studies have found that murine SCD MSCs exhibit altered gene signatures, reduced stem cell properties, and increased oxidative stress, leading to decreased HSC maintenance ability. The activation of Toll-like receptor-4 through p65 in MSCs further exacerbates MSC dysfunction in SCD.
