期刊
LAB ON A CHIP
卷 17, 期 14, 页码 2500-2507出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c7lc00339k
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资金
- Ramon y Cajal Programme
- Spanish Ministry of Economy and Competitiveness
- European Regional Development Fund (ERDF) [CTQ2013-46404-R, CTQ2016-77128-R, 2016 FI_B1 00136]
- Generalitat de Catalunya and Marti i Franques Programme from Universitat Rovira i Virgili [2013PMF-PIPF-16]
A novel low-cost, compact and sensitive paper-based platform for the accurate monitoring of glucose in biological fluids is presented. Paper-based working and reference electrodes are combined to build a whole potentiometric cell, which also fits a sampling module for simple and fast determination of glucose in a single drop of blood. The working electrode is built using a platinized filter paper coated with a Nafion membrane that entraps the enzyme glucose oxidase; the reference electrode is made by casting a polyvinylbutyral-based membrane onto a conductive paper. The system works by detecting the hydrogen peroxide generated as a result of the enzymatic reaction. Selectivity is achieved due to the permselective behaviour of Nafion, while a significant enhancement of the sensitivity is reached by exploiting the Donnan-coupled formal potential. Under optimum conditions, a sensitivity of -95.9 +/- 4.8 mV per decade in the 0.3-3 mM range is obtained. Validation of the measurements has been performed against standard methods in human serum and blood. Final integration with a wireless reader allows for truly in situ measurements with a less than 2 minute procedure including a two-point calibration, washing and measurement. This low-cost analytical device opens up new prospects for rapid diagnostic results in nonlaboratory settings.
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