Article
Microbiology
James C. Romero-Masters, Miranda Grace, Denis Lee, Joshua Lei, Melanie DePamphilis, Darya Buehler, Rong Hu, Ella Ward-Shaw, Simon Blaine-Sauer, Nathalie Lavoie, Elizabeth A. White, Karl Munger, Paul F. Lambert
Summary: Human papillomaviruses (HPVs) contribute to 5% of human cancers. Murine papillomavirus (MmuPV1) provides a powerful tool to study the roles of papillomavirus genes in pathogenesis arising from a natural infection. In this study, we confirmed the interaction between MmuPV1 E7 and PTPN14, and found that the intact PTPN14 binding site is essential for MmuPV1 E7 to promote virus-induced pathogenesis and delay epithelial differentiation.
Article
Virology
Kerry Dust, Michael Carpenter, Julie Chih-yu Chen, Chris Grant, Stuart McCorrister, Garret R. Westmacott, Alberto Severini
Summary: This study investigates the effects of HPV16 viral proteins E6 and E7 on the host proteome in human neonatal keratinocytes. The results show that the expression of E6 and E7 leads to dysregulation in pathways related to interferon response, inflammation, and DNA damage repair. The study also identifies potential biomarkers associated with HPV oncoproteins in primary keratinocytes.
Article
Oncology
Chiara Bertagnin, Lorenzo Messa, Matteo Pavan, Marta Celegato, Mattia Sturlese, Beatrice Mercorelli, Stefano Moro, Arianna Loregian
Summary: HPV-induced cancers are a major global health issue and lack specific therapeutic regimens. In this study, researchers identified a compound that could inhibit the interaction between the E7 protein and PTPN14, leading to a reduction in viability, proliferation, migration, and cancer-stem cell potential of HPV-positive cervical cancer cells. This compound also showed activity against cervical cancer cells transformed by different high-risk HPV genotypes, suggesting a potential broad-spectrum activity.
Article
Biology
Joshua Hatterschide, Paola Castagnino, Hee Won Kim, Steven M. Sperry, Kathleen T. Montone, Devraj Basu, Elizabeth A. White
Summary: This study found that the HPV oncoprotein E7 activates YAP1 and promotes its nuclear localization in basal epithelial cells. E7 activates YAP1 through binding and degradation of PTPN14, which plays a critical role in HPV persistence and carcinogenesis.
Article
Virology
Shuo Li, Mengxia Ye, Yonglin Chen, Quan Gong, Bing Mei
Summary: This study sheds light on the relationship between HPV-52 and cervical cancer, identifying variations in the E6-E7 genes and analyzing the protein's structure and selective pressure. Multiple single nucleotide variants were found in the E6 and E7 sequences of HPV-52, some impacting B cell epitopes.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Article
Microbiology
Ji Young Song, Young Min Park, Soon Yong Choi
Summary: The study found that HPV-2 can significantly decrease the expression levels of E-cadherin, which affects cell adhesion and may contribute to the formation of cutaneous warts.
JOURNAL OF MICROBIOLOGY
(2021)
Article
Virology
Sadhana M. Gupta, Himangi Warke, Hemangi Chaudhari, Padmaja Mavani, Rajshree D. Katke, Shilpa C. Kerkar, Jayanti Mania-Pramanik
Summary: This study aimed to evaluate HPV E6/E7 oncogene expression as markers for early identification of cervical cancer risk in women with minor cytological abnormalities and negative cytology. The results showed that E6 and E7 transcripts could serve as sensitive early predictors of cervical cancer risk.
JOURNAL OF MEDICAL VIROLOGY
(2022)
Article
Virology
Mengxia Ye, Shuo Li, Ping Luo, Xuan Tang, Quan Gong, Bing Mei
Summary: This study analyzed the variations and phylogenetic relationship of HPV-51 virus in central China, as well as predicted its immune epitopes. The results showed high prevalence and genetic variations of HPV-51 in the region. These findings are of great importance for future research on vaccines with wider coverage and the development of diagnostic probes.
JOURNAL OF MEDICAL VIROLOGY
(2022)
Article
Oncology
Jiang Zhu, Lingjing Chen, Qiaoli Zheng, Rui Han, Xianzhen Chen, Qiang Zhou, Hao Cheng
Summary: This study aimed to establish a reliable protocol for immunohistochemistry stains to detect HPV16 E7 protein and found a positive correlation between HPV16 E7 protein expression and malignancy grade.
Review
Virology
James C. Romero-Masters, Paul F. Lambert, Karl Munger
Summary: HPVs cause a wide range of human diseases, from benign warts to malignant cancers. The discovery of a murine papillomavirus that infects laboratory mice provides an opportunity to study HPV-induced malignant diseases. The E6 and E7 proteins encoded by high-risk HPVs contribute to HPV-induced cancers in multiple ways.
Article
Microbiology
Huimin Huang, Yongtao Li, Dongliang Li, Li Wang, Wenqiang Jiao, Yilin Bai, Gaiping Zhang
Summary: This study reveals the role of PTPN14 in PEDV infection. PTPN14 overexpression inhibits PEDV infection, while PTPN14 knockdown or phosphatase inhibitor treatment promotes PEDV proliferation. Furthermore, PTPN14 may regulate PEDV infection by dephosphorylating STAT3 in the cytoplasm.
VETERINARY MICROBIOLOGY
(2022)
Article
Medicine, Research & Experimental
Diane Bruyere, Patrick Roncarati, Alizee Lebeau, Thomas Lerho, Florian Poulain, Elodie Hendrick, Charlotte Pilard, Celia Reynders, Marie Ancion, Margaux Luyckx, Michael Renard, Yves Jacob, Jean-Claude Twizere, Raphael Peiffer, Olivier Peulen, Philippe Delvenne, Pascale Hubert, Alison McBride, Nicolas Gillet, Murielle Masson, Michael Herfs
Summary: HPV-positive cancers are usually associated with a favorable outcome due to high sensitivity to radiation therapy. However, the impact of viral E6/E7 oncoproteins on cellular radiosensitivity and host DNA repair is still largely speculative.
Article
Psychiatry
S. Dominguez-Alonso, A. Carracedo, C. Rodriguez-Fontenla
Summary: Autism Spectrum Disorders (ASD) is a group of neurodevelopmental disorders characterized by difficulties in social interaction and communication. The study uses a newly developed tool called eQTpLot to analyze the largest ASD GWAS data, along with GWAS summary statistics from other studies, to identify genes associated with ASD.
TRANSLATIONAL PSYCHIATRY
(2023)
Article
Biochemistry & Molecular Biology
Hye Seon Lee, Min Wook Kim, Kyeong Sik Jin, Ho-Chul Shin, Won Kon Kim, Sang Chul Lee, Seung Jun Kim, Eun-Woo Lee, Bonsu Ku
Summary: The interactions of the viral oncoprotein E7 with PTPN14 and PTPN21 are similar at the molecular level but play different physiological roles. PTPN21 exhibits pro-tumor activity and shows differences in biological consequences compared to PTPN14.
MOLECULES AND CELLS
(2021)
Article
Virology
Emily Montosa Nunes, Valeria Talpe-Nunes, Joao Simao Sobrinho, Silvaneide Ferreira, Vanesca de Souza Lino, Lara Termini, Gabriela Avila Fernandes Silva, Enrique Boccardo, Luisa Lina Villa, Laura Sichero
Summary: Studies suggest that HPV-18 variants from the A lineage have higher oncogenic potential compared to B variants, with uneven distribution in cervical adenocarcinomas and squamous cell carcinomas. Immortalized PHKs with HPV-18 A1 and B1 sublineages showed qualitative differences in colony formation, invasion ability, and resistance to apoptosis. Further investigation is needed to understand the mechanisms underlying these differences and their relevance to cervical cancer subtypes.
Article
Microbiology
Shyam Kumar Mishra, Prashant Basukala, Om Basukala, Keshab Parajuli, Bharat Mani Pokhrel, Basista Prasad Rijal
CURRENT MICROBIOLOGY
(2015)
Article
Microbiology
Om Basukala, Suruchi Mittal, Paola Massimi, Marco Bestagno, Lawrence Banks
Review
Biochemistry & Molecular Biology
Om Basukala, Vanessa Sarabia-Vega, Lawrence Banks
BIOLOGICAL CHEMISTRY
(2020)
Review
Virology
Om Basukala, Lawrence Banks
Summary: Infection with HPV begins with viral particles accessing basal cells in the epidermis through potential skin microtraumas. While HPV infections are typically cleared within 2 to 3 weeks by the host's immune response, persistent infections and compromised immune systems can lead to malignancy. Despite the strong association of HPV infection with cancer, therapeutic interventions against HPV-induced cancers remain a challenge, emphasizing the necessity for focused translational research.
Article
Virology
Ailyn C. Ramon, Om Basukala, Paola Massimi, Miranda Thomas, Yasser Perera, Lawrence Banks, Silvio E. Perea
Summary: This study investigated the physical interaction of CIGB-300 with HPV-E7 and its impact on CK2-mediated phosphorylation. The findings revealed that CIGB-300 binds to the N terminal region of E7 proteins of the HPV-16 type, reducing CK2-mediated phosphorylation of E7 and its binding to pRB. However, targeting of E7 phosphorylation by CIGB-300 seems to be dispensable for inducing cell death in HPV-18 cervical cancer-derived cells.
Article
Oncology
Maria Paula Dizanzo, Marina Bugnon Valdano, Om Basukala, Lawrence Banks, Daniela Gardiol
Summary: This study reveals the mechanisms behind the misexpression of PDZ-polarity proteins during Human Papillomavirus (HPV) infections, highlighting the importance of CKII-mediated regulation of E7 activities, and providing new insights into the joint action of HPV oncoproteins.
Article
Microbiology
Om Basukala, Oscar Trejo-Cerro, Michael P. Myers, David Pim, Paola Massimi, Miranda Thomas, Corrado Guarnaccia, David Owen, Lawrence Banks
Summary: HPV-E7 plays a crucial role in HPV-induced malignancy by targeting AP2 complex and modulating clathrin-mediated endocytosis, which may contribute to cellular transformation.
Meeting Abstract
Cell Biology
O. Basukala, A. Zine el Abidine, S. Mittal, P. Massimi, L. Banks
MOLECULAR BIOLOGY OF THE CELL
(2018)