期刊
JOURNAL OF VIROLOGICAL METHODS
卷 240, 期 -, 页码 14-20出版社
ELSEVIER
DOI: 10.1016/j.jviromet.2016.11.006
关键词
Reverse genetics; West Nile virus; Homologous recombination
资金
- JSPS KAKENHI Grant from the Ministry of Education, Culture, Sports, Sciences and technology of Japan [15K19069, 25304040, 26660220]
- Health Sciences Grants for Research on Emerging and Re-emerging Infectious Disease from the Ministry of Health, Labour and Welfare of Japan
- Grants-in-Aid for Scientific Research [16K15032, 25304040, 15K19069, 26660220] Funding Source: KAKEN
Reverse genetics systems facilitate investigation of many aspects of the life cycle and pathogenesis of viruses. However, genetic instability in Escherichia coli has hampered development of a reverse genetics system for West Nile virus (WNV). In this study, we developed a novel reverse genetics system for WNV based on homologous recombination in mammalian cells. Introduction of the DNA fragment coding for the WNV structural protein together with a DNA-based replicon resulted in the release of infectious WNV. The growth rate and plague size of the recombinant virus were almost identical to those of the parent WNV. Furthermore, chimeric WNV was produced by introducing the DNA fragment coding for the structural protein and replicon plasmid derived from various strains. Here, we report development of a novel system that will facilitate research into WNV infection. (C) 2016 Elsevier B.V. All rights reserved.
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