Article
Veterinary Sciences
Kayla M. Even, Angela M. Gaesser, Sarah A. Ciamillo, Renata L. Linardi, Kyla F. Ortved
Summary: Joint injury often leads to cartilage damage and posttraumatic inflammation, which can eventually cause osteoarthritis. This study aims to compare the immunomodulatory properties of bone marrow-derived mesenchymal stem cells (BM-MSCs) culture-expanded in different media supplemented with xenogen-free substitutes. The findings suggest that BM-MSCs cultured in equine serum (ES) demonstrate enhanced immunomodulatory abilities, while media containing platelet lysate (PL) may have therapeutic potential due to higher concentrations of growth factors. Further research is needed to determine the optimal xenogen-free supplement for optimizing BM-MSC performance.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Agriculture, Dairy & Animal Science
Usman Rashid, Evelyn Saba, Arfan Yousaf, Waleed Ahsan Tareen, Adeel Sarfraz, Man Hee Rhee, Mansur Abdullah Sandhu
Summary: The use of fetal bovine serum (FBS) in regenerative medicine has ethical and scientific concerns. This study found that autologous platelet lysate (A-PL) could be used as an alternative to FBS for culturing canine mesenchymal stem cells (cMSCs) without negatively affecting their proliferation and viability. Furthermore, the A-PL enhanced the genetic potential of the cMSCs for multilineage differentiation.
Article
Veterinary Sciences
Rudy Oyarzo, Ximena Valderrama, Francisca Valenzuela, Javiera Bahamonde
Summary: The viability of bovine fetal MSCs extracted from bone marrow (BM), adipose tissue (AT), and placenta (PT) was compared after exposure to different cryoprotectant solutions. BM-MSC exhibited significantly lower viability compared to AT-MSC and PT-MSC, with BM-MSC showing higher sensitivity to experimental conditions. BCL-2 expression was higher in BM-MSC, but the BAX/BCL-2 ratio did not differ among the cell types.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Pharmacology & Pharmacy
Ilenia Mastrolia, Andrea Giorgini, Alba Murgia, Pietro Loschi, Tiziana Petrachi, Valeria Rasini, Massimo Pinelli, Valentina Pinto, Francesca Lolli, Chiara Chiavelli, Giulia Grisendi, Maria Cristina Baschieri, Giorgio De Santis, Fabio Catani, Massimo Dominici, Elena Veronesi
Summary: In this study, a rabbit model was established to mimic human osteonecrosis of the femoral head (ONFH) and evaluate the autologous MSC-based treatment. Results showed that implantation of autologous BM-MSCs could restore the damaged tissue in rabbit femurs. Furthermore, the study found that osteonecrosis did not affect the behavior of MSCs.
Article
Materials Science, Multidisciplinary
Ran Li, Jiansheng Lin, Feng Zheng
Summary: Stem cell therapy provides a new approach for reconstructing central nervous system function and nerve regeneration. This study investigates the impact of autologous bone marrow mesenchymal stem cell transplantation on neural repair in an animal model of spinal cord injury. The combined treatment of graphene nanomaterials and autologous BMSC transplantation shows significant effects on neuropathic pain caused by spinal cord injury.
MATERIALS & DESIGN
(2022)
Article
Medicine, General & Internal
Hideaki Kagami, Minoru Inoue, Hideki Agata, Izumi Asahina, Tokiko Nagamura-Inoue, Masataka Taguri, Arinobu Tojo
Summary: By optimizing the cell-processing protocol, stable bone regeneration can be achieved with comparable quality and less fluctuation.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Cell Biology
Ju -Pi Li, Kang-Hsi Wu, Wan-Ru Chao, Yi-Ju Lee, Shun-Fa Yang, Yu-Hua Chao
Summary: Immune-mediated hematopoietic destruction plays a major role in idiopathic severe aplastic anemia. Mesenchymal stem cells (MSCs) have important immunomodulatory functions in the bone marrow niche. Dysfunction of MSCs is believed to be a major cause of immunological changes in SAA bone marrow.
Review
Biochemistry & Molecular Biology
Xing-An Wang, Ju-Pi Li, Kang-Hsi Wu, Shun-Fa Yang, Yu-Hua Chao
Summary: Aplastic anemia is a rare but life-threatening disease characterized by low blood cell count and reduced cellularity in the bone marrow. Mesenchymal stem cell dysfunction is believed to play a role in the development of the disease. This review provides a comprehensive understanding of the involvement of mesenchymal stem cells in acquired idiopathic aplastic anemia and discusses their clinical application. The pathophysiology of the disease, properties of mesenchymal stem cells, and results of mesenchymal stem cell therapy in animal models are also described. The authors anticipate that more patients with aplastic anemia can benefit from mesenchymal stem cell therapy in the future.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Yu-Hsun Chang, Kun-Chi Wu, Dah-Ching Ding
Summary: This study found that Exo(-)FBS-cultured HUCMSCs have similar characteristics to FBS-cultured HUCMSCs and showed better therapeutic effects in a mouse osteoarthritis model. These findings provide a basis for future clinical trials using Exo(-)FBS-cultured stem cells for the treatment of osteoarthritis.
Article
Veterinary Sciences
Aileen L. Rowland, Madison E. Burns, Gwendolyn J. Levine, Ashlee E. Watts
Summary: Research shows that there are significant differences between MSCs prepared with FBS and MSCs prepared without FBS, with FBS-prepared MSCs causing inflammation and antibody-mediated cytotoxicity. Additionally, the efficacy of FBS-prepared MSCs in treatment is reduced. Therefore, it is recommended to no longer use FBS in the preparation of MSCs.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Review
Immunology
Yanjing Huang, Mengdi Zhu, Zhuo Liu, Runan Hu, Fan Li, Yufan Song, Yuli Geng, Wenwen Ma, Kunkun Song, Mingmin Zhang
Summary: Premature ovarian failure is a common female reproductive disorder characterized by early menopause, increased gonadotropin levels, and estrogen deficiency. The use of bone marrow mesenchymal stem cells may offer potential for restoring ovarian function and fertility in POF patients.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Cell Biology
Jianhai Bi, Qiuchen Li, Zhigang Yang, Lei Cai, Tao Lv, Xun Yang, Li Yan, Xia Liu, Qian Wang, Xin Fu, Ran Xiao
Summary: Excessive consumption of high-fat diet (HFD) is a significant risk factor for many diseases, and induces low-grade systemic inflammation characterized by increased levels of pro-inflammatory cytokines, impairing the functions of mesenchymal stem cells (MSCs) like bone marrow MSCs. Specifically, continuous elevation of serum CXCL2 level in rats under HFD suppresses adipogenic potential, promotes migration and senescence of BMSCs via Rac1 activation, over-production of ELMO1 and ROS respectively.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Retraction
Biochemistry & Molecular Biology
Sabine Wislet-Gendebien, Emerence Laudet, Virginie Neirinckx, Philippe Alix, Pierre Leprince, Aneta Glejzer, Christophe Poulet, Benoit Hennuy, Lukas Sommer, Olga Shakhova, Bernard Rogister
Summary: A correction to this paper has been published.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Medicine, General & Internal
Izumi Asahina, Hideaki Kagami, Hideki Agata, Masaki J. Honda, Yoshinori Sumita, Minoru Inoue, Tokiko Nagamura-Inoue, Arinobu Tojo
Summary: This study evaluated the safety and efficacy of bone tissue engineering using BMSCs in patients with severe atrophic alveolar bone. The results showed good long-term stability and safety of the regenerated bone, suggesting potential application in clinical practice. However, individual variations were observed, highlighting the need for further studies with larger sample sizes to confirm the efficacy of BMSCs.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Biotechnology & Applied Microbiology
Yue Ke, Yu Ye, Jintao Wu, Yanxia Ma, Yuxin Fang, Fei Jiang, Jinhua Yu
Summary: A phosphoserine-loaded chitosan membrane was synthesized to improve the osteogenic effect of chitosan membranes. The incorporation of phosphoserine into chitosan membranes was confirmed and the phosphoserine-chitosan membranes significantly stimulated bone regeneration.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)