Review
Chemistry, Multidisciplinary
Amir Mohammad Maadani, Erfan Salahinejad
Summary: Bioceramics, particularly calcium phosphates, bioactive glasses, and crystalline silicates, are widely used biomaterials for tissue regeneration and therapeutic delivery. Polymeric coatings, such as polylactic acid (PLA) and poly(lactic-co-glycolic acid) (PLGA), have been extensively researched for enhancing the properties of ceramic tissue engineering scaffolds. Both PLA and PLGA can improve the mechanical behaviors of the scaffolds, but PLA has a greater influence on their biodegradability, bioactivity, and delivery kinetics. However, the biocompatibility response to this surface treatment is dependent on the specific conditions and needs further experimentation for accurate determination.
JOURNAL OF CONTROLLED RELEASE
(2022)
Article
Engineering, Biomedical
Caroline D. Hoemann, Javier Rodriguez Gonzalez, Jessica Guzman-Morales, Gaoping Chen, Ebrahim Jalali Dil, Basil D. Favis
Summary: This study found that non-inflammatory chitosan coatings promoted cartilage infiltration into and over a bioplastic scaffold, and were compatible with trabecular bone integration. Compared to drill-only defects, the 83-99-PCL scaffold induced angiogenesis and slight bone resorption, with the smallest change in bone volume fraction.
BIOACTIVE MATERIALS
(2022)
Article
Biochemistry & Molecular Biology
Sebastian Blatt, Daniel G. E. Thiem, Andreas Pabst, Bilal Al-Nawas, Peer W. Kaemmerer
Summary: The combination of bone substitute materials (BSMs) with platelet-rich fibrin (PRF) showed significant positive pro-angiogenic effects, particularly with alloplastic and xenogeneic materials, leading to increased vessel formation in vivo. This demonstrates the enhanced angiogenetic potential of BSMs when combined with PRF, suggesting potential for bony regeneration in clinical applications.
Article
Materials Science, Biomaterials
Lidong Huang, Weiyu Gong, Guibin Huang, Jingyi Li, Jilin Wu, Yanmei Dong
Summary: Bioactive glasses (BG) are commonly used in bone defect repair for their osteoinductivity and osteoconductivity. However, the early angiogenesis of BG may not be sufficient to support new bone formation in large-sized defects. Photobiomodulation (PBM) therapy, known for promoting early angiogenesis, may enhance the angiogenesis of BG and improve the bone repair. In this study, BG combined with PBM showed additive effects on bone regeneration, enhancing cell proliferation, gene expression, mineralization, new bone formation, and bone reconstruction.
REGENERATIVE BIOMATERIALS
(2023)
Article
Orthopedics
Junlei Li, Fang Cao, Bin Wu, Jiahui Yang, Wenwu Xu, Weidan Wang, Xiaowei Wei, Ge Liu, Dewei Zhao
Summary: This study successfully immobilized VEGF on the surface of degradable magnesium through polydopamine, giving it angiogenic bioactivity. This method has the potential to promote bone tissue regeneration and play a significant role in the clinical treatment of large bone defects.
JOURNAL OF ORTHOPAEDIC TRANSLATION
(2021)
Review
Biochemistry & Molecular Biology
Sundaravadhanan Lekhavadhani, Abinaya Shanmugavadivu, Nagarajan Selvamurugan
Summary: The biomimetics of scaffolds in bone regeneration is crucial for promoting osteogenic differentiation. Changes in porous characteristics of scaffolds may affect their physicochemical and biological properties. Chitosan-based scaffolds have shown potential in promoting osteogenesis through supporting cell attachment and proliferation and the formation of mineralized bone matrix. This article discusses the correlation between porous properties of chitosan-based scaffolds and osteogenesis and angiogenesis, as well as methods to overcome the mechanical limitations of chitosan.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Chemistry, Multidisciplinary
Jin Zhang, Dongmei Tong, Honghai Song, Renjie Ruan, Yifu Sun, Yandai Lin, Jun Wang, Linxi Hou, Jiayong Dai, Jianxun Ding, Huanghao Yang
Summary: Engineering a biomimetically hierarchical scaffold can improve bone tissue regeneration by facilitating the balance of the immune system and bone metabolism. The scaffold exhibits strong immunomodulatory, intensive angiogenic, weak osteoclastogenic, and superior osteogenic abilities, providing a valuable foundation and inspiration for large-scale bone defect repair.
ADVANCED MATERIALS
(2022)
Article
Polymer Science
Miguelangel Moncayo-Donoso, Gustavo A. Rico-Llanos, Diego A. Garzon-Alvarado, Jose Becerra, Rick Visser, Marta R. Fontanilla
Summary: This study fabricated uni- and multidirectional collagen sponges and evaluated their microstructural, physicochemical, mechanical and biological characteristics. The unidirectional sponges showed higher tensile strength, Young's modulus and liquid absorption capacity, but pore orientation had some influence on cell attachment and differentiation. Both types of scaffolds supported bone formation in an ectopic rat model, with histological analysis confirming the influence of pore orientation on the osteogenic process.
Review
Biochemistry & Molecular Biology
Zhaozhao Ding, Weinan Cheng, Md Shipan Mia, Qiang Lu
Summary: Silk, a natural fibrous polymer, shows potential in regenerative medicine and bone tissue engineering. It is processed into scaffolds, hydrogels, and films to facilitate bone regeneration. Bioactive factors and stem cells are introduced to create osteogenic microenvironments for directing bone regeneration.
MACROMOLECULAR BIOSCIENCE
(2021)
Article
Engineering, Biomedical
Qingli Liu, Wenling Dai, Yongli Gao, Longpeng Dong, Hengxing Jia, Shikui Li, Likun Guo, Yujiang Fan, Xingdong Zhang
Summary: The physicochemical properties of collagen-based hydrogels and co-culture system have synergistic effects on cartilage formation. The spreading and proliferation of cells are regulated by the degradation rate and stiffness of hydrogels. Co-culture cells in collagen-based hydrogels significantly improve chondrogenesis.
ACTA BIOMATERIALIA
(2022)
Review
Biochemistry & Molecular Biology
Parisa Khayambashi, Janaki Iyer, Sangeeth Pillai, Akshaya Upadhyay, Yuli Zhang, Simon D. Tran
Summary: Tissue engineering has a long history in regenerative medicine, and methods such as cell-encapsulated hydrogels and exosomes can enhance the regenerative potential of various tissues, with hydrogels being the most user-friendly, economical, and accessible material for encapsulation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Arlinda Ljoki, Tanzila Aslam, Tina Friis, Ragnhild G. Ohm, Gunnar Houen
Summary: In this study, a co-culture assay with human umbilical vein endothelial cells (HUVECs) and normal human dermal fibroblasts (NHDFs) was used to investigate the effects of selected angiogenesis inhibitors on the differentiation and network formation of HUVECs in vitro. The results showed that neutralizing VEGF and inhibiting VEGFR2 resulted in the formation of HUVEC clusters, while other inhibitors of the VEGF signaling pathways did not have this effect. Additionally, glucocorticoid receptor activation was found to be linked to angiogenesis and HUVEC differentiation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Engineering, Biomedical
Mohammed A. Al-Baadani, Lihua Xu, Kexin Cai, Kendrick Hii Ru Yie, Yiding Shen, Abdullrahman M. Al-Bishari, Bilal A. Al-Shaaobi, Pingping Ma, Xinkun Shen, Jinsong Liu
Summary: The study aimed to develop a tissue regeneration membrane that can improve damaged areas by promoting angiogenesis and inducing osteogenesis. The researchers fabricated a hierarchical polycaprolactone/gelatin co-electrospinning membrane that incorporated substance P and simvastatin. In vitro and in vivo experiments confirmed the membrane's ability to enhance cell homing, revascularization, and bone regeneration.
MATERIALS TODAY BIO
(2023)
Review
Materials Science, Biomaterials
Yiping Song, Ning Wang, Huixin Shi, Dan Zhang, Qiang Wang, Shu Guo, Shude Yang, Jia Ma
Summary: Bone tissue engineering, supported by scaffold, seed cells, and bioactive molecules, offers new hope for treating bone defects. Adipose-derived stem cells (ADSCs) are favored by researchers due to their abundant sources and multi-differentiation potentials. However, there is a lack of systematic theoretical basis for selecting suitable biomaterials loaded with ADSCs.
REGENERATIVE BIOMATERIALS
(2023)
Article
Anatomy & Morphology
Nima Beheshtizadeh, Yazdan Asgari, Noushin Nasiri, Ali Farzin, Mohammad Ghorbani, Nasrin Lotfibakhshaiesh, Mahmoud Azami
Summary: The purpose of tissue engineering is to stimulate damaged tissues to recover their function using chemical agents, cells, and biocompatible materials. Choosing the right protein as a growth factor is crucial for creating artificial tissues. This study identified PTGS2 and FGF18 as potential growth factors for bone tissue engineering applications, while TEK requires further investigation.
Article
Engineering, Biomedical
Bin Ma, Ming Li, Sabine Fuchs, Iris Bischoff, Alexander Hofmann, Ronald E. Unger, Charles J. Kirkpatrick
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2020)
Article
Dentistry, Oral Surgery & Medicine
Sarah Al-Maawi, James L. Rutkowski, Robert Sader, C. James Kirkpatrick, Shahram Ghanaati
JOURNAL OF ORAL IMPLANTOLOGY
(2020)
Article
Oncology
Laura Anspach, Roman Tsaryk, Larissa Seidmann, Ronald E. Unger, Caren Jayasinghe, Nektaria Simiantonaki, C. James Kirkpatrick, Felicitas Proels
JOURNAL OF PATHOLOGY
(2020)
Article
Orthopedics
David Grevenstein, Andreas Mamilos, Volker H. Schmitt, Tanja Niedermair, Willi Wagner, C. James Kirkpatrick, Christoph Brochhausen
Summary: The study analyzed histological and immunohistological findings from five patients who underwent spheroid-based autologous chondrocyte transplantation after traumatic lesions of the knee, showing excellent histological results in terms of hyaline articular cartilage regeneration. This indicates that spheroid-based autologous chondrocyte transplantation is a promising strategy for treating traumatic lesions of the knee's articular cartilage.
KNEE SURGERY SPORTS TRAUMATOLOGY ARTHROSCOPY
(2021)
Article
Dentistry, Oral Surgery & Medicine
Anna Maria Tanneberger, Sarah Al-Maawi, Carlos Herrera-Vizcaino, Anna Orlowska, Alica Kubesch, Robert Sader, C. J. Kirkpatrick, Shahram Ghanaati
Summary: The study characterized the cellular reaction to a xenogeneic resorbable collagen membrane of porcine origin in Wistar rats over 30 days. It was found that the membrane induced a large number of mononuclear cells, including lymphocytes, macrophages, and fibroblasts, as well as multinucleated giant cells (MNGCs) expressing specific markers. The significant increase in MNGC number correlated with a high rate of vascularization compared to the control group, suggesting a potential role for MNGCs in biomaterial degradation.
CLINICAL ORAL INVESTIGATIONS
(2021)
Review
Immunology
Ignaz Wessler, Charles James Kirkpatrick
INTERNATIONAL IMMUNOPHARMACOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Susana G. Guerreiro, Ronald E. Unger, Nuno M. F. S. A. Cerqueira, Anne Sartoris, Maria J. Martins, Mario A. Barbosa, Raquel Soares, Pedro L. Granja, Charles J. Kirkpatrick
Summary: The study found that cocultures of fibroblasts and human endothelial cells stimulate collagen synthesis and growth factors production by fibroblasts, ultimately affecting the formation and distribution of microvessel-like structures in cell cultures. Areas with activated fibroblasts and high alkaline phosphatase (ALP) activity were also observed in the cocultures, indicating the impact of ALP on collagen proteins' aggregation, cell migration, and microvessel assembly. These findings suggest that bioinformatics and coculture systems are valuable tools for investigating the involvement of proteins like collagen and ALP in angiogenesis.
JOURNAL OF CELLULAR BIOCHEMISTRY
(2021)
Article
Chemistry, Physical
Samuel Ebele Udeabor, Carlos Herrera-Vizcaino, Robert Sader, C. James Kirkpatrick, Sarah Al-Maawi, Shahram Ghanaati
Article
Cell & Tissue Engineering
Eva Dohle, Andreas Scherrieble, Michael Doser, Sarah Al-Maawi, Martin Hoss, Martin Dauner, Robert Sader, Charles James Kirkpatrick, Shahram Ghanaati
TISSUE ENGINEERING PART C-METHODS
(2020)
Article
Oncology
Cristina L. Cotarelo, Annemarie Zschoeck-Manus, Marcus Schmidt, Arno Schad, Christoph Brochhausen, Charles James Kirkpatrick, Sonja Thaler
Summary: The intraoperative method combining touch imprint cytology (TIC) and subsequent frozen section (FS) analysis is highly reliable for detecting SLN macrometastases, allowing accurate identification of patients at high risk for extended axillary involvement during surgery and for accurate histological diagnosis of negative SLN.
CLINICAL & EXPERIMENTAL METASTASIS
(2021)
Article
Engineering, Biomedical
Sarah Al-Maawi, Mike Barbeck, Carlos Herrera Vizcaino, Rainer Egli, Robert Sader, Charles James Kirkpatrick, Marc Bohner, Shahram Ghanaati
Summary: The biological response of calcium phosphates is influenced by the presence of an alkaline layer on their surface during thermal treatment. Thermally treated alpha-TCP and beta-TCP granules stimulate fewer multinucleated giant cells and are more resistant to degradation compared to non-thermally treated granules. Heat treatment of alpha-TCP and beta-TCP granules shifts the reaction from foreign body response to physiological response, by reducing the number of induced multinucleated giant cells and enhancing degradation resistance.
ACTA BIOMATERIALIA
(2021)
Article
Chemistry, Physical
Sarah Al-Maawi, Xuejiu Wang, Robert Sader, Werner Goetz, Antonella Motta, Claudio Migliaresi, Charles James Kirkpatrick, Shahram Ghanaati
Summary: This study analyzed the long-term polarization pattern of macrophages and multinucleated giant cells (MNGCs) in the implantation areas of biomaterials, and found sustained expression of pro-inflammatory markers in SF-induced MNGCs over 180 days. This highlights the importance of tailored physicochemical properties to avoid extensive inflammatory reactions for clinical success.
Article
Biochemistry & Molecular Biology
Thanuja D. K. Herath, Leonardo Saigo, Benoit Schaller, Anis Larbi, Swee Hin Teoh, Charles James Kirkpatrick, Bee Tin Goh
Summary: This study showed that autologous neutrophils may have a positive effect on promoting new bone formation, providing potential clinical benefits for bone regeneration in oral and maxillofacial surgery.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Engineering, Biomedical
Sarah Al-Maawi, Sandra Rother, Norbert Halfter, Karen M. Fiebig, Juliane Moritz, Stephanie Moeller, Matthias Schnabelrauch, Charles James Kirkpatrick, Robert Sader, Hans-Peter Wiesmann, Dieter Scharnweber, Vera Hintze, Shahram Ghanaati
Summary: This study aimed to functionalize a collagen-based scaffold with high-sulfated hyaluronan and investigate its properties in vitro and in vivo. The results showed that covalent binding of high-sulfated hyaluronan to collagen released only a marginal amount of the hyaluronan, while electrostatic binding released more. The covalent binding scaffolds showed reduced release of vascular endothelial growth factor and transforming growth factor beta 1, but increased release of interleukin-8 and epithelial growth factor compared to other scaffolds. In vivo, the covalent binding scaffolds reduced the proinflammatory macrophage response and did not induce multinucleated giant cell formation, while maintaining a stable structure and integration into the implantation region. This functionalized collagen scaffold has potential as a stable biomaterial for full-thickness skin regeneration.
BIOACTIVE MATERIALS
(2022)
Article
Oncology
Linda A. Liang, Thomas Einzmann, Arno Franzen, Katja Schwarzer, Gunther Schauberger, Dirk Schriefer, Kathrin Radde, Sylke R. Zeissig, Hans Ikenberg, Chris J. L. M. Meijer, Charles J. Kirkpatrick, Heinz Koelbl, Maria Blettner, Stefanie J. Klug
Summary: The study compared the strategies of stand-alone HPV testing and cotesting for cervical cancer screening in a population-based study in Germany. The results showed that cotesting had higher sensitivity but also resulted in more false positive results and colposcopy referrals compared to stand-alone HPV testing.
CANCER EPIDEMIOLOGY BIOMARKERS & PREVENTION
(2021)