期刊
JOURNAL OF THE KOREAN PHYSICAL SOCIETY
卷 71, 期 11, 页码 752-757出版社
KOREAN PHYSICAL SOC
DOI: 10.3938/jkps.71.752
关键词
Soybean; Mutant; Proton beam; Morphological response
资金
- National Research Foundation of Korea (NRF) - Korean government (MSIP: Ministry of Science, ICT and Future Planning) [NRF-2015M2B2A4028156]
- Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (IPET) through the Agri-Bio Industry Technology Development Program
- Ministry of Agriculture, Food and Rural Affairs (MAFRA), Republic of Korea [315025-03-1-HD060]
- National Research Foundation of Korea [2015M2B2A4028156] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
The present study aimed to evaluate the morphological effects of proton beam irradiation on the seed germination, seedling survival, and plant growth of soybean. Seeds of three Korean elite cultivars (Kwangankong, Daepungkong, and Pungsannamulkong) were irradiated with a 57-MeV proton beam in the range of 50 - 400 Gy. The germination rates of all the varieties increased to > 95%; however, the survival rates were significantly reduced. At doses of > 300 Gy irradiation, the Daepungkong, Kwangankong, and Pungsannamulkong cultivars exhibited 39, 75, and 71% survival rates, respectively. In addition, plant height and the fresh weight of shoots and roots were significantly decreased by doses of > 100 Gy irradiation, as were the dry weights of the shoots and roots. However, SPAD values increased with increasing doses of irradiation. Abnormal plants with atypically branched stems, modified leaves, and chlorophyll mutations were observed. Based on the survival rate, plant growth inhibition, and mutation frequency, it appears that the optimum dosage of proton beam irradiation for soybean mutation breeding is between 250 and 300 Gy.
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