期刊
JOURNAL OF THE FORMOSAN MEDICAL ASSOCIATION
卷 116, 期 7, 页码 542-548出版社
ELSEVIER TAIWAN
DOI: 10.1016/j.jfma.2016.09.001
关键词
c-KIT; male infertility; spermatogenesis; stem cell factor; varicocele
资金
- National Natural Science Foundation of China [81373650, 81401192]
- Shanxi Natural Science Foundation [2009011057-3, 2012011045-4]
Background/Purpose: Varicocele (VC) is considered by the World Health Organization as the main cause of male infertility. Studies have shown that VC can affect spermatogenesis and then result in male infertility. But the exact mechanism by which VC affects spermatogenesis is still unclear. Stem cell factor (SCF) and c-KIT receptor are crucial molecules during spermatogenesis in testis. This study aims to investigate whether SCF/c-KIT signaling is involved in the pathophysiology of VC on spermatogenesis. Methods: Rat models of VC were built (n = 13), and sham-operated rats were used as controls (n = 8). The seminiferous tubules of the testis were observed with hematoxylin and eosin staining, expression of SCF was analyzed via enzyme-linked immunosorbent assay and Western blot, and expression of c-KIT was assessed with Western blot and immunofluorescence. Results: Compared with controls, the seminiferous epithelium was disorganized and had significantly fewer cells in the testes of rats with VC. Expression of SCF increased in testes of VC rats, while expression of c-KIT was decreased. Conclusion: These results suggest that sperm counts in seminiferous epithelium are affected by VC, and the SCF/c-KIT system is aberrantly expressed in VC testis, which could be involved in male infertility caused by VC. Copyright (C) 2016, Formosan Medical Association. Published by Elsevier Taiwan LLC.
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