Review
Microbiology
Yasuhito Funahashi
Summary: Recent advances in immunosuppressive therapy have improved renal transplantation outcomes by reducing acute rejection incidence. BK virus nephropathy has emerged as a significant cause of graft dysfunction, progressing from viruria to viremia and associated nephropathy. Diagnosis of BK virus nephropathy involves histological examination and categorization of disease severity to guide treatment strategies.
Article
Chemistry, Physical
Jaehyeon Hwang, Younghun Jung, Seokoh Moon, Seokhyeon Yu, Hyunseok Oh, Soomin Kim, Kyeong Won Kim, Jeong Hyeon Yoon, Jihwan Chun, Sang Jick Kim, Woo-Jae Chung, Dae-Hyuk Kweon
Summary: This study demonstrates the engineering of a nanodisc to enhance the antiviral activity of antibodies. The nanodisc can form a complex with antibodies, which can enter infected cells and inhibit viral replication and spread.
Article
Virology
Francesca Colavita, Giulia Matusali, Valentina Mazzotta, Aurora Bettini, Daniele Lapa, Silvia Meschi, Massimo Francalancia, Carmela Pinnetti, Licia Bordi, Klizia Mizzoni, Sabrina Coen, Enrico Girardi, Francesco Vaia, Emanuele Nicastri, Andrea Antinori, Fabrizio Maggi
Summary: This study describes the short-term kinetics of the antibody response in MPXV-infected patients during the 2022 multicountry outbreak. The results show that IgG, IgM, and IgA antibodies were detected as early as 4 days after symptom onset, while neutralizing antibodies were detectable after 1 week. There were no significant differences in antibody levels regardless of smallpox vaccination status, HIV positivity, or disease severity. Lower levels of IgM and IgG were observed in patients treated with antivirals.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Review
Genetics & Heredity
Margherita Borriello, Diego Ingrosso, Alessandra Fortunata Perna, Angela Lombardi, Paolo Maggi, Lucia Altucci, Michele Caraglia
Summary: Poliomavirus BK virus (BKV) is a highly infective virus that can cause asymptomatic infections. In renal transplanted recipients, BKV can lead to severe complications. Current treatments and prophylaxis options are limited, but increasing surveillance and early diagnosis show promise in improving outcomes.
Article
Chemistry, Multidisciplinary
Jinrong Long, Changxiao Yu, Yiming Cao, Yiqi Miao, Huisheng Sun, Zhen Zhang, Jierui Mai, Xin Wang, Yingying Mao, Hongwei Li, Jing Yang, Shengqi Wang
Summary: Rabies is a deadly viral disease and vaccinations are the most effective way to prevent and control it. Researchers have developed an mRNA-based vaccine that can be produced quickly and in large quantities. This vaccine has shown robust immune responses and long-term protection in mice and dogs.
Review
Immunology
Vincent Dussupt, Kayvon Modjarrad, Shelly J. Krebs
Summary: The unprecedented Zika outbreak in the Americas from 2015-2016 led to global concern and accelerated the deployment of vaccine and therapeutic countermeasures. Several potent neutralizing antibodies against Zika and related flaviviruses have been discovered in recent years. Structure-based approaches have helped identify species-specific and cross-protective epitopes with therapeutic value.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Multidisciplinary Sciences
Noriaki Sato, Keita P. Mori, Kaoru Sakai, Hitomi Miyata, Shinya Yamamoto, Takashi Kobayashi, Hironori Haga, Motoko Yanagita, Yasushi Okuno
Summary: BK polyomavirus-associated nephropathy can occur in kidney transplant recipients undergoing immunosuppressive treatment, and it is potentially linked to the development of renal cell carcinoma. This study analyzed gene expression profiles of renal biopsy samples and identified a gene cluster specifically upregulated in BK polyomavirus-associated nephropathy, which was associated with the prognosis of renal cell carcinoma.
Review
Microbiology
Fenghao Peng, Naijing Hu, Yingjun Liu, Cong Xing, Longlong Luo, Xinying Li, Jing Wang, Guojiang Chen, He Xiao, Chenghua Liu, Beifen Shen, Jiannan Feng, Chunxia Qiao
Summary: Smallpox is an infectious disease caused by the variola virus, with a high mortality rate. Although smallpox was declared eradicated in 1980, the potential threat of bioterrorism has led to resumed research on the treatment and prevention of smallpox.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Zongmin Zhao, Jayoung Kim, Vinny Chandran Suja, Neha Kapate, Yongsheng Gao, Junling Guo, Vladimir R. Muzykantov, Samir Mitragotri
Summary: This study demonstrates that using red blood cells (RBCs) as carriers for adeno-associated virus (AAV) can improve its tissue-targeted transduction and enable repetitive administration. RBC-anchored AAVs show efficient transduction in the lungs and lead to enhanced target gene expression compared to free AAVs.
Article
Biotechnology & Applied Microbiology
Yanshan Dai, Uma Kavita, Margit H. Lampen, Sander Gielen, Glen Banks, Paul Levesque, Alexander Kozhich, Renuka Pillutla, Yan Zhang, Vibha Jawa, Leonard Adam
Summary: Pre-existing neutralizing antibodies to adeno-associated virus (AAV) may reduce the effectiveness of AAV-based therapies. A study on different pig strains showed that most pigs had a good response to AAV1, AAV5, AAV8, and AAV9, suggesting that these strains are suitable for gene therapy studies. Pre-screening for circulating AAV antibodies could be helpful before including pigs in studies.
HUMAN GENE THERAPY
(2022)
Article
Microbiology
Poonsook Keelapang, Romchat Kraivong, Rojjanaporn Pulmanausahakul, Rungtawan Sriburi, Eakachai Prompetchara, Jutamart Kaewmaneephong, Nicha Charoensri, Pattarakul Pakchotanon, Thaneeya Duangchinda, Piyanan Suparattanagool, Prasit Luangaram, Promsin Masrinoul, Juthathip Mongkolsapaya, Gavin Screaton, Kiat Ruxrungtham, Prasert Auewarakul, Sutee Yoksan, Prida Malasit, Chunya Puttikhunt, Chutitorn Ketloy, Nopporn Sittisombut
Summary: This study describes a blockade-of-binding assay to determine antibodies recognizing particular epitopes in the envelope of dengue virus. The assay showed moderate to strong correlations between epitope-blocking activities and virus-neutralizing antibody titers for each of the four dengue serotypes using blood samples from infected or immunized macaques.
MICROBIOLOGY SPECTRUM
(2023)
Article
Virology
Guosong Wang, Pengfei Huang, Junping Hong, Rao Fu, Qian Wu, Ruiqi Chen, Lina Lin, Qiangyuan Han, Honglin Chen, Yixin Chen, Ningshao Xia
Summary: The ELISPOT assay based on the efficient detection of influenza virus and neutralizing antibodies is rapid, accurate, and high-throughput, serving as a powerful tool for quantification in the research and development of drugs and vaccines against influenza. The study demonstrated a good correlation with traditional PFU and PRNT methods, making it a valuable alternative in influenza studies.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Article
Cell Biology
Panke Qu, Julia N. Faraone, John P. Evans, Yi-Min Zheng, Claire Carlin, Mirela Anghelina, Patrick Stevens, Soledad Fernandez, Daniel Jones, Ashish R. Panchal, Linda J. Saif, Eugene M. Oltz, Baoshan Zhang, Tongqing Zhou, Kai Xu, Richard J. Gumina, Shan-Lu Liu
Summary: This study demonstrates the nearly complete escape of XBB.1.5, CH.1.1, and CA.3.1 variants from neutralizing antibodies stimulated by mRNA vaccine or BA.4/5 wave infection, but neutralization can be rescued by a bivalent booster containing BA.5. CH.1.1 and CA.3.1 also show strong immune escape from monoclonal antibody S309. Furthermore, the spike proteins of XBB.1.5, CH.1.1, and CA.3.1 exhibit enhanced fusogenicity and processing compared to BA.2.
Article
Veterinary Sciences
Qi Wu, Zhixian Lin, Jinsen Wu, Kun Qian, Hongxia Shao, Jianqiang Ye, Aijian Qin
Summary: The study developed a peptide enzyme-linked immunosorbent assay (pELISA) as a possible alternative to the neutralization test for evaluating the immune response to IBV vaccine. The pELISA could indirectly evaluate neutralizing antibody titers against different types of IBV in all tested sera, with a coefficient of 0.83 for neutralizing antibody titers. The pELISA has the potential to detect antibodies against different types of IBV in all tested sera and surveillance the infection of IBV.
BMC VETERINARY RESEARCH
(2021)
Article
Immunology
Christina R. DeMaso, Lovkesh Karwal, Melissa Zahralban-Steele, David Dominguez, Zhang-Li Springer, Maima Kaiser, Sunil Palani, Tim Rindfleisch, Kelly Bohning, Greg Hather, Subash Das, Mayuri Sharma, Hansi J. Dean
Summary: The study found that the TAK-003 vaccine can induce broad type-specific and cross-reactive neutralizing antibody response.
JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Geriatrics & Gerontology
Cedric Villain, Marie Metzger, Sophie Liabeuf, Aghiles Hamroun, Solene Laville, Nicolas Mansencal, Christian Combe, Denis Fouque, Luc Frimat, Christian Jacquelinet, Maurice Laville, Carole Ayav, Serge Briancon, Roberto Pecoits-Filho, Thierry Hannedouche, Benedicte Stengel, Ziad A. Massy
Summary: This study suggests that aging does not appear to modify the beneficial effects of RASi on major CKD outcomes or their potential adverse effects.
JOURNAL OF THE AMERICAN MEDICAL DIRECTORS ASSOCIATION
(2022)
Article
Urology & Nephrology
Dorian Nezam, Raphael Porcher, Francois Grolleau, Pauline Morel, Dimitri Titeca-Beauport, Stanislas Faguer, Alexandre Karras, Justine Solignac, Noemie Jourde-Chiche, Francois Maurier, Hamza Sakhi, Khalil El Karoui, Rafik Mesbah, Pierre Louis Carron, Vincent Audard, Didier Ducloux, Romain Paule, Jean-Fracois Augusto, Julien Aniort, Aurelien Tiple, Cedric Rafat, Severine Beaudreuil, Xavier Puechal, Pierre Gobert, Ziad Massy, Catherine Hanrotel, Stephane Bally, Nihal Martis, Cecile-Audrey Durel, Geoffroy Desbuissons, Pascal Godmer, Aurelie Hummel, Francois Perrin, Antoine Neel, Claire De Moreuil, Tiphaine Goulenok, Dominique Guerrot, Steven Grange, Aurelie Foucher, Alban Deroux, Carole Cordonnier, Celine Guilbeau-Frugier, Anne Modesto-Segonds, Dominique Nochy, Laurent Daniel, Anissa Moktefi, Marion Rabant, Loic Guillevin, Alexis Regent, Benjamin Terrier
Summary: The study found that PLEX did not improve the primary outcome in the entire population, but identified a subset of patients who could benefit from PLEX. However, these findings need to be validated before being used in clinical decision making.
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
(2022)
Letter
Urology & Nephrology
Dorian Nezam, Raphael Porcher, Francois Grolleau, Benjamin Terrier, French Vasculitis Study Grp
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
(2022)
Article
Biochemistry & Molecular Biology
Raphael Carapito, Ismail Aouadi, Martin Verniquet, Meiggie Untrau, Angelique Pichot, Thomas Beaudrey, Xavier Bassand, Sebastien Meyer, Loic Faucher, Juliane Posson, Aurore Morlon, Irina Kotova, Florent Delbos, Alexandre Walencik, Alice Aarnink, Anne Kennel, Caroline Suberbielle, Jean-Luc Taupin, Benedict M. Matern, Eric Spierings, Nicolas Congy-Jolivet, Arnaud Essaydi, Peggy Perrin, Antoine Blancher, Dominique Charron, Nezih Cereb, Myriam Maumy-Bertrand, Frederic Bertrand, Valerie Garrigue, Vincent Pernin, Laurent Weekers, Maarten Naesens, Nassim Kamar, Christophe Legendre, Denis Glotz, Sophie Caillard, Marc Ladriere, Magali Giral, Dany Anglicheau, Caner Suesal, Seiamak Bahram
Summary: This study investigates the role of the MICA gene in a French multicenter cohort of kidney transplants and finds that MICA mismatches are associated with decreased graft survival. Both before and after transplantation, anti-MICA donor-specific antibodies are strongly associated with increased antibody-mediated rejection. This effect is synergetic with anti-HLA DSA. De novo-developed anti-MICA DSA are the most harmful and associated with reduced graft survival. Evaluation of MICA matching and immunization is warranted for identifying high-risk transplant rejection and loss.
Article
Engineering, Manufacturing
Theodoros Evgeniou, Mathilde Fekom, Anton Ovchinnikov, Raphael Porcher, Camille Pouchol, Nicolas Vayatis
Summary: This study presents a framework that combines epidemiological and machine learning models to predict the risk of ICU treatment for individuals infected with COVID-19. Simulation results based on French data show that policies considering clinical risk predictions can relax isolation restrictions earlier without overwhelming the ICU capacity.
PRODUCTION AND OPERATIONS MANAGEMENT
(2022)
Article
Multidisciplinary Sciences
Viet-Thi Tran, Raphael Porcher, Isabelle Pane, Philippe Ravaud
Summary: About 10% of people infected by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) experience post COVID-19 disease. This study analyzed data from a French disease cohort and found that among patients symptomatic after 2 months, 85% still reported symptoms one year after their symptom onset. Symptoms showed different trends over time, with some decreasing, some remaining stable, and some increasing in prevalence. The study provides important insights into the natural history of post COVID-19 disease.
NATURE COMMUNICATIONS
(2022)
Article
Hematology
David Marx, Arnaud Dupuis, Anita Eckly, Anne Molitor, Jerome Olagne, Guy Touchard, Sihem Kaaki, Cecile Ory, Anne-Laure Faller, Benedicte Gerard, Melanie Cotter, Lisa Westerberg, Marton Keszei, Bruno Moulin, Christian Gachet, Sophie Caillard, Seiamak Bahram, Raphael Carapito
Summary: This study reports a gain-of-function variant in the WAS gene associated with both the hematological phenotype of X-linked neutropenia (neutropenia, macrothrombocytopenia) and renal disease (proteinuria, renal failure).
Article
Health Care Sciences & Services
Dimitris Mavridis, Adriani Nikolakopoulou, Irini Moustaki, Anna Chaimani, Raphael Porcherd, Isabelle Boutron, Philippe Ravaud
Summary: This study presents graphical methods to group interventions and uses conjoint analysis to place weights on outcomes based on stakeholders' preferences. The results provide valuable information about the clustering of interventions. Grouping interventions helps decision makers identify optimal options in terms of benefit-risk balance and choose interventions from the best cluster based on other factors such as cost and implementation.
JOURNAL OF CLINICAL EPIDEMIOLOGY
(2023)
Review
Food Science & Technology
Maria Lavilla, Pilar Domingo-Calap, Sandra Sevilla-Navarro, Amaia Lasagabaster
Summary: Ingestion of food or water contaminated with pathogenic bacteria can lead to serious diseases. The One Health approach is important in preventing the spread of diseases between animals, humans, and the environment, especially antibiotic-resistant bacteria. Bacteriophages have gained importance as effective alternatives for treating bacterial diseases and ensuring food safety.
Review
Hematology
Jerome Lambert, Etienne Lengline, Raphael Porcher, Rodolphe Thiebaut, Sarah Zohar, Sylvie Chevret
Summary: In the past decade, it has become common practice to provide rapid answers and early patient access to innovative treatments in the absence of randomized clinical trials. This trend is particularly important in oncology when evaluating new targeted therapies. This article provides guidelines for the critical appraisal of comparisons or conducting single-arm trials, using the example of ciltacabtagene autoleucel for the treatment of relapsed or refractory multiple myeloma.
Article
Hematology
Florie Brion Bouvier, Raphael Porcher
Summary: The preferred approach to compare two treatments is a randomized controlled trial (RCT) which ensures similarity between groups and allows causal conclusions. When RCTs are not possible, observational data can be used to infer treatment effectiveness under certain assumptions, using regression modeling or propensity score methods. Target trial emulation principles are also discussed.
BEST PRACTICE & RESEARCH CLINICAL HAEMATOLOGY
(2023)
Article
Virology
Vicente Arnau, Wladimiro Diaz-Villanueva, Jorge Mifsut Benet, Paula Villasante, Beatriz Beamud, Paula Mompo, Rafael Sanjuan, Fernando Gonzalez-Candelas, Pilar Domingo-Calap, Maria Dzunkova
Summary: The environmental impact of uncultured phages is influenced by their life cycle, which can be predicted by comparing their genomic signatures to those of their hosts. This study used oligonucleotide-based methods to successfully discriminate between lytic and lysogenic phages and predict their relationships with host bacteria.
Article
Medicine, Research & Experimental
Sylvain Mayeur, Benoit Lhermitte, Justine Gantzer, Anne Molitor, Tristan Stemmelen, Sebastien Meyer, Aline Kolmer, Jean-Emmanuel Kurtz, Seiamak Bahram, Raphael Carapito
Summary: Exome sequencing of a patient with postchemotherapy thymic teratoma revealed 19 shared somatic mutations among the mature teratoma, melanocytic neuroectodermal tumor (MNT), and metastatic anaplastic MNT components. Enrichment analysis indicated the involvement of known cancer-related genes such as KRAS, TP53, ERBB4, and KDR in the development of anaplastic MNT transformation. This study provides valuable insights into the molecular characteristics of MNT and highlights the importance of understanding these tumors for improved therapeutics.
COLD SPRING HARBOR MOLECULAR CASE STUDIES
(2023)
Article
Microbiology
Robby Concha-Eloko, Pilar Barberan-Martinez, Rafael Sanjuan, Pilar Domingo-Calap
Summary: The emergence of antibiotic-resistant bacteria is a serious threat to global health, and new treatments are needed. This study presents three new Klebsiella phages with a broad host range, which hold potential for combating infections by multiple capsular types of Klebsiella pneumoniae.
MICROBIOLOGY SPECTRUM
(2023)
Review
Infectious Diseases
Assilina Parfut, Elodie Laugel, Sarah Baer, Gaelle Gonzalez, Yves Hansmann, Marie-Josee Wendling, Samira Fafi-Kremer, Aurelie Velay
Summary: Tick-borne encephalitis (TBE) is caused by the tick-borne encephalitis virus (TBEV) transmitted by infected Ixodes ricinus ticks. TBEV-Eu is endemic in 27 European countries, and there has been an increase in TBE incidence, even in countries with low endemicity. Children with TBE may experience severe forms and long-term cognitive impairment. This review comprehensively describes TBEV incidence, transmission routes, clinical manifestations, imaging findings, and long-term outcomes in children, as well as vaccination recommendations in European countries.
INFECTIOUS DISEASES NOW
(2023)
