Article
Chemistry, Multidisciplinary
Dao Thi Nguyet Nga, Quan Doan Mai, Ha Anh Nguyen, Nguyen Le Nhat Trang, Pham Minh Khanh, Nguyen Quang Hoa, Vu Dinh Lam, Van-Tuan Hoang, Anh-Tuan Le
Summary: A functional ternary substrate consisting of MnO2 nanosheets, graphene oxide (GO) nanosheets, and plasmonic Ag nanoparticles (e-AgNPs) was developed for surface-enhanced Raman scattering (SERS) sensing systems. The substrate showed enhanced SERS sensing signal for organic chemicals without the need for chemical bonds, and improved sensing performance compared to individual components.
Review
Oncology
Luca Guerrini, Eduardo Garcia-Rico, Ana O'Loghlen, Vincenzo Giannini, Ramon A. Alvarez-Puebla
Summary: Exosomes serve as important cancer biomarkers, while nanoplasmonic methods incorporating surface-enhanced Raman spectroscopy technique have become effective tools for detecting and discriminating exosomes. The main challenge lies in achieving rapid, sensitive, and cost-effective analysis of exosomes.
Article
Chemistry, Analytical
Qingnan Zhao, Hai Wang, Wenji Jiang, Huimin Gao, Sisi Wen, Xin Feng, Qiong Wu, Chengyan He, Youliang Zhu, Lianghai Hu, Bing Zhao, Wei Song
Summary: Understanding the catalytic process in an acetic acid-sodium acetate buffer environment is crucial for designing efficient nanozymes. This study investigated the lattice restructuration of Au-LaNiO3-delta nanofibers after acidification and revealed the role of carboxyl group insertion in enhancing oxidase-like activity and protecting vacancies. The strategy offers potential for constructing an efficient nanozyme-linked immunosorbent assay system.
ANALYTICAL CHEMISTRY
(2022)
Article
Engineering, Biomedical
Qiong Wu, Qin Ding, Wanzun Lin, Youliang Weng, Shangyuan Feng, Rong Chen, Chuanben Chen, Sufang Qiu, Duo Lin
Summary: This study combined surface-enhanced Raman spectroscopy with proteomics to analyze exosomes secreted from radioresistant nasopharyngeal cancer cells. Specific protein expression variations associated with radioresistance formation were identified, including collagen alpha-2 (I) chain (COL1A2), which is associated with a favorable prognosis in NPC. A deep learning model-based diagnostic model was developed to accurately identify exosomes from the radioresistant group. This research demonstrates the potential of exosomes as a biomarker for predicting radioresistance and provides a rapid and sensitive liquid biopsy method for personalized clinical NPC treatment.
ADVANCED HEALTHCARE MATERIALS
(2022)
Review
Biochemistry & Molecular Biology
Antonio Sasso, Angela Capaccio, Giulia Rusciano
Summary: This review focuses on the recent studies conducted by our group in the past decade, where novel nanofabrication techniques were successfully applied to SERS and TERS experiments for studying bio-systems and molecular species of environmental interest.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Kirill Khabarov, Emiliia Filalova, Messan Nouraldeen, Ekaterina Kameneva, Andrey Musaev, Sergei Tikhonov, Victor Ivanov
Summary: In this study, the influence of agglomeration degree of nanoparticles on the signal enhancement of surface-enhanced Raman spectroscopy (SERS) was investigated. It was found that structures formed mainly from non-agglomerated nanoparticles can enhance the SERS signal better. Aerosol nanoparticles modified by pulsed laser radiation show better results compared to thermally modified nanoparticles.
Article
Chemistry, Analytical
Yu Fukunaga, Makoto Harada, Tetsuo Okada
Summary: Freezing is proposed as an efficient way to enhance sensitivity and reproducibility in SERS measurements, maximizing signal intensity and preparing a high-performance SERS platform through simple methods.
Article
Chemistry, Multidisciplinary
Niclas S. Mueller, Emanuel Pfitzner, Yu Okamura, Georgy Gordeev, Patryk Kusch, Holger Lange, Joachim Heberle, Florian Schulz, Stephanie Reich
Summary: Research shows that plasmonic supercrystals, with a high density of hotspots, are an excellent platform for enhancing vibrational spectra by increasing the cross section of Raman scattering and infrared absorption. The study demonstrates that the intensity of surface-enhanced Raman scattering inside the crystal is uniform and predictable, with supercrystal polaritons inducing resonances in the enhanced cross section for both Raman scattering and infrared absorption. The enhancement of infrared absorption within supercrystals is attributed to the combined effects of hotspot formation and the excitation of standing polariton waves.
Article
Materials Science, Multidisciplinary
Zhinan Yu, Zhengkun Wang, Jie Zhang
Summary: The study discussed the Raman enhancement mechanism of optical fibers decorated with silver nanoparticles as SERS substrates, focusing on the LSPR of AgNPs and cavity enhancement of a silver capillary. Optimal parameters for coating AgNPs and the formation mechanism based on temperature and velocity field distributions were explored. Experimental results demonstrated a detection limit down to 10(-11) mol/L, with a total enhancement factor of approximately 10(9) and an additional cavity-coupled enhancement effect.
OPTICAL MATERIALS EXPRESS
(2022)
Article
Chemistry, Analytical
Samuel Adesoye, Saqer Al Abdullah, Anjali Kumari, Gayani Pathiraja, Kyle Nowlin, Kristen Dellinger
Summary: This study proposes a novel method for biomolecular detection using surface-enhanced Raman scattering (SERS) technique to address the limitations of current detection methods. By coating gold (Au) precursor onto zinc oxide (ZnO), the signal enhancement is significantly improved and applied for molecular identification of exosomes.
Article
Chemistry, Analytical
Xiao-Xia Zhou, Rui Liu, Li-Teng Hao, Jing-Fu Liu
Summary: The accumulation of micro- and nanoplastics in the environment is a major global issue, with concerns about potential harm to wildlife. Identification of nanoplastics in environmental samples remains a challenge, but surface enhanced Raman spectroscopy (SERS) has been shown as a feasible approach to quickly identify and analyze trace polystyrene (PS) nanoplastics. The proposed method has the potential to further study the occurrence, formation, and transports of nanoplastics in the natural environment.
Article
Chemistry, Analytical
Di Liu, Huimin Gao, Wenji Jiang, Su Yan, Hao Liu, Junjie Chen, Sisi Wen, Wei Zhang, Xu Wang, Bing Zhao, Wei Song
Summary: In this study, three-dimensional Ag aerogel-supported Hg single-atom catalysts were used as an efficient SERS substrate for monitoring enhanced oxidase-like reactions. The effect of Hg2+ concentrations on the SERS properties of 3D Hg/Ag aerogel networks was investigated, and an optimized addition of Hg2+ resulted in specific enhancement. The formation of Ag-supported Hg SACs was confirmed using HAADF-STEM imaging and XPS measurement, and the oxidase-like catalytic mechanism of Hg/Ag SACs was revealed using DFT. This research provides a mild synthetic strategy for fabricating Ag aerogel-supported Hg single atoms with promising prospects in various catalytic fields.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Physical
Han-Kyu Choi, Sang-Min Park, Jeeyoon Jeong, Hankyul Lee, Gyu Jin Yeon, Dai-Sik Kim, Zee Hwan Kim
Summary: In this study, a simple photolithographic method was demonstrated to generate highly sensitive SERS hot spot structures at desired positions. The solid-state dewetting of an Ag thin film using a continuous-wave laser resulted in the formation of closely packed assemblies of hemispherical Ag nanoislands with substantial plasmonic-field enhancement. These hot spot structures can be utilized as position-specific chemical-sensing elements in integrated analytical devices.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2022)
Article
Chemistry, Analytical
Robert A. Hunter, Meshach Asare-Werehene, Aseel Mandour, Benjamin K. Tsang, Hanan Anis
Summary: The study introduces a new method of diagnosing chemoresistance in ovarian cancers by quantifying tumor-derived exosomes and cisplatin drugs within them using surface enhanced Raman scattering and support vector machine models. The method can differentiate between histological subtypes of ovarian cancer and their chemoresponsiveness with over 90% accuracy.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Review
Chemistry, Analytical
Irene Calderon, Luca Guerrini, Ramon A. Alvarez-Puebla
Summary: Surface-enhanced Raman spectroscopy (SERS) combines nanotechnology with traditional Raman spectroscopy to create an ultra-sensitive and highly specific analytical tool, utilized in various advanced applications such as biosensing platforms for nucleic acids (NAs) and precision engineering of nanomaterials using structural DNA nanotechnology.
Article
Chemistry, Multidisciplinary
Hao Wu, Lu Zhang, Jinfan Yang, Ruonan Bo, Hongxu Du, Kai Lin, Dalin Zhang, Mythili Ramachandran, Yingbin Shen, Yangxi Xu, Xiangdong Xue, Zhao Ma, Aaron Raymond Lindstrom, Randy Carney, Tzu-Yin Lin, Yuanpei Li
ADVANCED FUNCTIONAL MATERIALS
(2020)
Article
Optics
Haojie Ji, Valeria Nava, Yu Yang, James W. Chan
Article
Biochemical Research Methods
Marcos A. Soares de Oliveira, Michael Campbell, Alaa M. Afify, Eric C. Huang, James W. Chan
BIOMEDICAL OPTICS EXPRESS
(2020)
Article
Chemistry, Multidisciplinary
Tatu Rojalin, Hanna J. Koster, Juanjuan Liu, Rachel R. Mizenko, Di Tran, Sebastian Wachsmann-Hogiu, Randy P. Carney
Article
Instruments & Instrumentation
Tatu Rojalin, Dexter Antonio, Ambarish Kulkarni, Randy P. Carney
Summary: SERS is a powerful technique for sensitive label-free analysis, but automation progress is limited by manual sample preparation. Current automation efforts focus on downstream processing of collected data, but there is a need to develop algorithms to simplify and accelerate data collection. Machine learning-assisted methods at the acquisition stage can serve as a useful building block for point-of-care SERS diagnostic platforms.
APPLIED SPECTROSCOPY
(2022)
Article
Biotechnology & Applied Microbiology
Rachel R. Mizenko, Terza Brostoff, Tatu Rojalin, Hanna J. Koster, Hila S. Swindell, Gary S. Leiserowitz, Aijun Wang, Randy P. Carney
Summary: The study revealed distinct patterns in tetraspanin expression of EVs from different sources, with flow cytometry measurements showing some inconsistencies. Non-specific tetraspanin capture can bias multiplexed tetraspanin profiles, impacting diagnostic sensitivity for tumor-associated EV surface markers. The findings provide insights into protein expression heterogeneity of EVs, informing better EV capture and detection platforms for diagnostic or downstream use.
JOURNAL OF NANOBIOTECHNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Silvia Hilt, Ruiwu Liu, Izumi Maezawa, Tatu Rojalin, Hnin H. Aung, Madhu Budamagunta, Ryan Slez, Qizhi Gong, Randy P. Carney, John C. Voss
Summary: This study synthesized a class of bifunctional stilbenes to modulate the toxicity and oxidative stress of amyloid beta oligomers (AβO) in Alzheimer's disease. The findings suggest that this bifunctional approach has the potential to counteract the molecular pathogenesis of Alzheimer's disease in a powerful and synergistic manner.
FRONTIERS IN CHEMISTRY
(2022)
Article
Chemistry, Physical
Hanna J. Koster, Tatu Rojalin, Randy P. Carney, Hannah J. O'Toole, Kwan Lun Chiu
Summary: Surface enhanced Raman scattering (SERS) is a powerful tool for vibrational spectroscopy that enhances chemical sensitivity by several orders of magnitude compared to spontaneous Raman scattering. However, synthesizing robust and uniform SERS substrates quickly and easily remains a challenge.
COLLOID AND INTERFACE SCIENCE COMMUNICATIONS
(2022)
Article
Microbiology
Rachel R. Mizenko, Terza Brostoff, Kenneth Jackson, Patricia A. Pesavento, Randy P. Carney
Summary: Feline calicivirus (FCV) is a significant cause of respiratory disease in cats and has implications for human medical research. This study explores the role of extracellular vesicles (EVs) in FCV infection and highlights the importance of coisolates during viral purification.
MICROBIOLOGY SPECTRUM
(2022)
Review
Nanoscience & Nanotechnology
Jugal Suthar, Marissa Taub, Randy P. Carney, Gareth R. Williams, Stefan Guldin
Summary: Extracellular vesicles (EVs) are widely regarded as a valuable source of biomarkers for human health and disease. However, their small size and complexity present challenges for effective characterization. This has led to the development of novel EV biosensing techniques to overcome these limitations.
WILEY INTERDISCIPLINARY REVIEWS-NANOMEDICINE AND NANOBIOTECHNOLOGY
(2023)
Article
Nanoscience & Nanotechnology
Lalithasri Ramasubramanian, Harsha Jyothi, Leora Goldbloom-Helzner, Brandon M. Light, Priyadarsini Kumar, Randy P. Carney, Diana L. Farmer, Aijun Wang
Summary: This study validates lipid rafts as a functional biomaterial for synthesizing biomimetic nanovesicles and shows their potential in promoting neurogenesis and angiogenesis.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Multidisciplinary Sciences
Hanna J. Koster, Antonio Guillen-Perez, Juan Sebastian Gomez-Diaz, Maria Navas-Moreno, Andrew C. Birkeland, Randy P. Carney
Summary: Raman spectroscopy shows great promise for liquid biopsy cancer detection and diagnosis as it is rapid, label-free, non-destructive, and requires little to no sample preparation. By fusing the Raman spectra of plasma and saliva, higher diagnostic performance can be achieved, demonstrating the ability of Raman spectroscopy to provide additive value across different biofluid sources.
SCIENTIFIC REPORTS
(2022)
Article
Medicine, Research & Experimental
Dake Hao, Lu Lu, Hengyue Song, Yixin Duan, Jianing Chen, Randy Carney, Jian Jian Li, Ping Zhou, Jan Nolta, Kit S. Lam, J. Kent Leach, Diana L. Farmer, Alyssa Panitch, Aijun Wang
Summary: A new generation of collagen-binding extracellular vesicles (EVs) was developed to improve their retention and therapeutic efficacy. The results showed that these collagen-binding EVs had longer in situ retention, suppressed inflammatory responses, and enhanced tissue regeneration.
Article
Chemistry, Multidisciplinary
Hanna J. Koster, Tatu Rojalin, Alyssa Powell, Dina Pham, Rachel R. Mizenko, Andrew C. Birkeland, Randy P. Carney
Summary: This study confirms that common EV isolation methods yield variable lipoprotein content, and dual isolation methods are necessary for successful separation of EVs from different lipoprotein classes. Additionally, disease state is the main driver for distinguishing EV samples, largely unaffected by the choice of isolation method.
Article
Chemistry, Multidisciplinary
Meruyert Imanbekova, Sorina Suarasan, Tatu Rojalin, Rachel R. Mizenko, Silvia Hilt, Meghna Mathur, Paula Lepine, Michael Nicouleau, Nguyen-Vi Mohamed, Thomas M. Durcan, Randy P. Carney, John C. Voss, Sebastian Wachsmann-Hogiu
Summary: This study used Raman spectroscopy to analyze the biochemical composition of Aβ peptide in extracellular vesicles (EVs) associated with Alzheimer's disease. The results suggest that Aβ associated with secreted EVs has an alpha-helical secondary structure and the size of a monomer or small oligomer. Additionally, altered fatty acid chain lengths were identified in EVs carrying Aβ, potentially affecting the fluidity of the EV membrane.
NANOSCALE ADVANCES
(2021)
