期刊
JOURNAL OF PROTEOME RESEARCH
卷 16, 期 4, 页码 1383-1390出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jproteome.6b00034
关键词
G-PTM-D; post-translational modification discovery; proteomics; database search
资金
- National Institutes of Health from the National Institute of General Medical Sciences [R01GM103315, R01GM114292]
- National Institutes of Health from the National Institute on Deafness and Other Communication Disorders [R01 DC010777, R01 DC010777-S1]
A new global post-translational modification (PTM) discovery strategy, G-PTM-D, is described. A proteomics database containing UniProt-curated PTM information is supplemented with potential new modification types and sites discovered from a first-round search of mass spectrometry data with ultrawide precursor mass tolerance. A second-round search employing the supplemented database conducted with standard narrow mass tolerances yields deep coverage and a rich variety of peptide modifications with high confidence in complex unenriched samples. The G-PTM-D strategy represents a major advance to the previously reported G-PTM strategy and provides a powerful new capability to the proteomics research community.
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