期刊
JOURNAL OF PROTEOME RESEARCH
卷 16, 期 7, 页码 2653-2659出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jproteome.7b00249
关键词
electron-transfer dissociation; activated-ion; photoactivation; infrared photons; intact proteins; top-down proteomics
资金
- Thermo Fisher Scientific
- NIH [R35 GM119110, R21 GM118341]
- NIH Predoctoral to Postdoctoral Transition Award [F99 CA212454]
Here we report the first demonstration of near complete sequence coverage of intact proteins using activated ion-electron transfer dissociation (AI-ETD), a method that leverages concurrent infrared photoactivation to enhance electron-driven dissociation. AI-ETD produces mainly c/z-type product ions and provides comprehensive (77-97%) protein sequence coverage, outperforming HCD, ETD, and EThcD for all proteins investigated. AI-ETD also maintains this performance across precursor ion charge states, mitigating charge-state dependence that limits traditional approaches.
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