Article
Plant Sciences
Yuliya Genievskaya, Alibek Zatybekov, Saule Abugalieva, Yerlan Turuspekov
Summary: In this study, a collection of 406 barley accessions were evaluated for powdery mildew resistance, and seven QTLs associated with resistance were identified. Haplotype analysis revealed three haplotypes associated with total resistance and one haplotype associated with high severity of the disease. These findings provide valuable information for resistance analysis, trait pyramiding, and marker-assisted selection in barley.
Article
Agronomy
Jerzy H. Czembor, Elzbieta Czembor
Summary: Out of 129 barley landraces from Turkey, 19 showed resistance to powdery mildew. One line (5583-1-4) exhibited resistance against all 19 isolates used. These new sources of highly effective powdery mildew resistance in barley landraces from Turkey could be successfully utilized in breeding programs.
Article
Agronomy
Paula Moolhuijzen, Cynthia Ge, Elzette Palmiero, Simon R. R. Ellwood
Summary: Gene expression analysis revealed that RBgh2 plays a role in cAMP/G-protein-coupled signalling and innate immunity in barley powdery mildew adult plant resistance. RBgh2 is a major effect adult plant resistance gene in barley powdery mildew, showing different underlying genetic factors compared to conventional resistance genes. The resistance mechanism of RBgh2 involves temporary genome-wide gene expression changes and constitutive changes, especially at the RBgh2 locus, inducing defence-related genes associated with innate immunity and pathogen recognition.
THEORETICAL AND APPLIED GENETICS
(2023)
Article
Agronomy
Antonin Dreiseitl
Summary: The aim of this study is to identify genes for powdery mildew resistance in spring barley varieties bred for human consumption. Fifteen resistance genes, including the nonspecific resistance gene Mlo, were found. The use of Mlo gene is recommended for barley varieties earmarked for human consumption.
Article
Plant Sciences
Judit Kolozsvarine Nagy, Ildiko Schwarczinger, Lorant Kiraly, Renata Bacso, Attila L. Adam, Andras Kunstler
Summary: This study investigates the effects of short- and long-term heat stress on barley resistance to powdery mildew infection, finding that heat stress negatively affects resistance and enhances susceptibility in barley cultivars.
Article
Agronomy
Jerzy H. Czembor, Elzbieta Czembor, Radoslaw Suchecki, Nathan S. Watson-Haigh
Summary: This study identified marker-trait associations for powdery mildew, barley brown rust, and stem rust resistance in European barley accessions. The results will be used to establish a Polish Gene Bank platform for breeding programs and serve as a valuable resource for breeding resistant barley varieties.
Article
Plant Sciences
Hongbo Yuan, Cong Jin, Hongcui Pei, Lifang Zhao, Xue Li, Jiali Li, Wanting Huang, Renchun Fan, Wende Liu, Qian-Hua Shen
Summary: The study reveals that the effector protein CSEP0027 of the barley mildew fungus interacts with barley catalase HvCAT1 to regulate host immunity and likely reactive oxygen species homeostasis, promoting fungal virulence during barley infection.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Samar M. Esmail, Diego Jarquin, Andreas Boerner, Ahmed Sallam
Summary: This study evaluated the NB and PM resistance of 122 diverse barley genotypes from 34 countries and found high genetic variation among the genotypes. No significant correlation was found between PM and NB resistance, but 21 genotypes immune to both diseases were identified. Furthermore, GWAS identified SNP markers associated with increased resistance to PM and NB.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2023)
Article
Multidisciplinary Sciences
Shengnan Li, Dexing Lin, Yunwei Zhang, Min Deng, Yongxing Chen, Bin Lv, Boshu Li, Yuan Lei, Yanpeng Wang, Long Zhao, Yueting Liang, Jinxing Liu, Kunling Chen, Zhiyong Liu, Jun Xiao, Jin-Long Qiu, Caixia Gao
Summary: Disruption of susceptibility genes in crops can confer disease resistance, but often leads to undesired effects. This study identifies a mutant wheat variety with a targeted deletion in a specific gene that retains crop growth and yields while conferring robust resistance to powdery mildew.
Article
Plant Sciences
Cynthia Ge, Elzette Wentzel, Nola D'Souza, Kefei Chen, Richard P. Oliver, Simon R. Ellwood
Summary: Barley powdery mildew is a major disease that breeders have traditionally controlled using dominant, pathogen race-specific resistance genes. However, continuous selection pressure and pathogen mutation have led to the need for alternative solutions. Three novel major-effect adult plant resistance genes have been identified from landraces, offering opportunities for breeding programs to develop robust mildew resistance.
Article
Plant Sciences
Xiaodong Zhang, Pei Wang, Yafei Wang, Lian Hu, Xiwen Luo, Hanping Mao, Baoguo Shen
Summary: This study explores the use of information technology in detecting crop diseases, and proposes a method based on hyperspectral-terahertz technology for detecting cucumber powdery mildew. The research focuses on the effective establishment of hyperspectral data, spectral preprocessing, feature wavelength selection, and discriminant model establishment. Additionally, the study also investigates the preprocessing of terahertz data, the screening of terahertz effective spectrum, and the establishment of a sparse representation classification model. The findings demonstrate that the detection of cucumber powdery mildew can be achieved using hyperspectral and terahertz technology.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Agronomy
Zha Sang, Minjuan Zhang, Wang Mu, Haizhen Yang, Chunbao Yang, Qijun Xu
Summary: This study explored the phytohormone levels and transcriptome of a Bgh susceptible and resistant hulless barley genotypes, revealing higher levels of plant hormones in the resistant genotype. Transcriptome analysis identified more differentially expressed genes in the resistant genotype in defense-related pathways. Changes in hormone levels drove downstream gene expression changes in plant-hormone signaling, aiding the resistant genotype in performing better under Bgh infection.
Article
Plant Sciences
Lior Gur, Yigal Cohen, Omer Frenkel, Ron Schweitzer, Meir Shlisel, Moshe Reuveni
Summary: The foliar application of potassium phosphate fertilizer and micronutrients can effectively reduce the incidence and severity of powdery mildew on grape leaves and berries. These fertilizers are as effective as fungicides and can increase the production of anti-fungal and antioxidant metabolites, providing further control of the disease and improving wine quality.
Article
Biochemistry & Molecular Biology
Stefan Engelhardt, Adriana Trutzenberg, Michaela Kopischke, Katja Probst, Christopher McCollum, Johanna Hofer, Ralph Hueckelhoven
Summary: Successful pathogens benefit from host cellular processes. The small monomeric G-protein RACB is required for susceptibility of Blumeria hordei to fungal penetration in barley. RACB can interact with the host protein RIC157, promoting fungal penetration into barley epidermal cells.
PLANT MOLECULAR BIOLOGY
(2023)
Article
Agronomy
Xiaona Liang, Xiaomin Bie, Yuliang Qiu, Ke Wang, Zujun Yang, Yuqi Jia, Zhiying Xu, Mei Yu, Lipu Du, Zhishan Lin, Xingguo Ye
Summary: Genetic transformation is a common method to improve traits and study gene function in wheat, but its efficiency is influenced by factors including powdery mildew infection. In this study, PM resistant lines with Pm21 and PmV genes were backcrossed to the susceptible variety Fielder, and five transgenic lines were developed and evaluated for their agronomic traits and regeneration ability. Among them, FL347, WT48, NT89, and NT154 showed similar or superior regeneration and transformation ability compared to Fielder, making them suitable materials for wheat gene transfer and genome editing studies.
Article
Agronomy
Tomasz Warzecha, Roman Bathelt, Edyta Skrzypek, Marzena Warchol, Jan Bocianowski, Agnieszka Sutkowska
Summary: The environmental conditions have an impact on the growth and productivity of crop plants. Among the various factors, drought stress is identified as one of the major threats to plant productivity. This research focused on evaluating the physiological, biochemical, and agronomic parameters of OMA plants under soil drought conditions. The results showed that most of the examined lines exhibited reduced photosynthetic pigment content, PSII photosystem activity, and yield-related traits under drought stress. However, two lines (9 and 78b) were found to retain high yielding potential compared to the commercial cv. Bingo under drought stress.
Article
Genetics & Heredity
Aras Turkoglu, Kamil Haliloglu, Seyyed Abolgahasem Mohammadi, Ali Ozturk, Parisa Bolouri, Guller Ozkan, Jan Bocianowski, Alireza Pour-Aboughadareh, Bita Jamshidi
Summary: This study investigated the molecular diversity and population structure of 120 Turkish bread wheat genotypes using 120 microsatellite markers. The results showed that wheat genotypes have rich genetic diversity, which can be used to improve wheat genotypes for food security.
Article
Chemistry, Multidisciplinary
Dariusz Piesik, Jacek Lyczko, Krzysztof Krawczyk, Magdalena Gantner, Jan Bocianowski, Veronika Ruzsanyi, Chris A. A. Mayhew
Summary: This study aims to determine the similarity between the profile of green leaf volatiles (GLVs) naturally produced by undamaged plants and those provoked by chemical stimulants. It also investigates the influence of sex and mating status on the reproductive choices of Apion miniatum beetles. Additionally, it assesses the use of chemically stimulated GLV emissions as a means to attract pests for biological control of Rumex confertus.
APPLIED SCIENCES-BASEL
(2023)
Article
Agronomy
Fatih Demirel, Baris Eren, Abdurrahim Yilmaz, Aras Turkoglu, Kamil Haliloglu, Gniewko Niedbala, Henryk Bujak, Bita Jamshidi, Alireza Pour-Aboughadareh, Jan Bocianowski, Kamila Nowosad
Summary: This study aimed to determine the correlations between morphological traits of various wheat species and demonstrated the application of MARS and CHAID algorithms in wheat-derived data sets. The MARS algorithm was found to be the best model for grain yield prediction and outperformed the CHAID algorithm. This research showed how MARS and CHAID algorithms can help wheat breeders describe complex interaction effects more accurately.
Article
Plant Sciences
Hassan Zali, Ali Barati, Alireza Pour-Aboughadareh, Ahmad Gholipour, Shirali Koohkan, Akbar Marzoghiyan, Jan Bocianowski, Henryk Bujak, Kamila Nowosad
Summary: The main objective of this study was to evaluate and select superior barley genotypes based on grain yield and phenotypic traits using a new proposed selection index (SIIG). The results showed that the SIIG index has the potential to identify genotypes with high yield and desirable traits.
Article
Plant Sciences
Alireza Pour-Aboughadareh, Habibollah Ghazvini, Seyed Shahriyar Jasemi, Solaiman Mohammadi, Sayed Alireza Razavi, Mehrdad Chaichi, Marefat Ghasemi Kalkhoran, Hassan Monirifar, Hamid Tajali, Asadollah Fathihafshjani, Jan Bocianowski
Summary: The present study aimed to analyze the effect of genotype-by-environment interaction on barley in cold regions of Iran and identify superior genotypes. G2 and G20 were found to be superior genotypes in terms of yield and stability, with G20 considered as the ideal genotype. ARK1 and KAJ2 were identified as ideal environments for testing advanced genotypes. Therefore, it is recommended to include the high-yielding and stable G20 genotype in breeding programs for cold climates in Iran.
Article
Genetics & Heredity
Julia Spychala, Agnieszka Tomkowiak, Aleksandra Noweiska, Roksana Bobrowska, Jan Bocianowski, Michal Ksiazkiewicz, Aleksandra Sobiech, Michal Tomasz Kwiatek
Summary: The main efforts in common wheat breeding focus on yield, grain quality, and resistance to biotic and abiotic stresses. Rust diseases, including leaf rust caused by Puccinia triticina, are a major threat to global wheat cultivation. The study investigated the early transcriptomic responses of two major wheat APR genes, Lr34 and Lr67, and three complementary miRNAs, tae-miR9653b, tae-miR9773, and tae-miR9677b, to inoculation with P. triticina. The expression profiles of the APR genes and miRNAs varied between varieties and time-points, but the expression of Lr34 and Lr67 genes in studied cultivars was significantly higher than in a control line carrying susceptible alleles.
Article
Chemistry, Multidisciplinary
Jan Bocianowski, Kamila Nowosad, Henryk Bujak
Summary: Knowledge of the genetic potential and variation in parental forms is crucial for shortening the breeding process and achieving desired traits in hybrids. The effects of heterosis and specific combining ability can be predicted through selection of genetically diverse parental forms. Preliminary studies suggest that selecting phenotypically equal but genetically diverse parental forms is the best approach for obtaining favorable hybrids.
APPLIED SCIENCES-BASEL
(2023)
Article
Chemistry, Multidisciplinary
Fatih Demirel, Aras Turkoglu, Kamil Haliloglu, Baris Eren, Guller Ozkan, Pinar Uysal, Alireza Pour-Aboughadareh, Agnieszka Lesniewska-Bocianowska, Bita Jamshidi, Jan Bocianowski
Summary: This study investigated the effects of mammalian sex hormones on DNA damage and DNA methylation in wheat plants, and found that these hormones can alter the genomic stability and cytosine methylation status of wheat plants.
APPLIED SCIENCES-BASEL
(2023)
Article
Agronomy
Kamila Nowosad, Jan Bocianowski, Farzad Kianersi, Alireza Pour-Aboughadareh
Summary: The assessment of 1000-kernel weight is crucial for determining maize grain yield, and understanding its genetic mechanisms is important for improving overall performance. This study focused on 26 doubled-haploid maize lines and evaluated genotype by environment interaction, selection of stable lines, and estimation of additive and additive-additive gene interaction effects. Results indicated significant effects of genotype and environment, as well as genotype by environment interaction, on 1000-kernel weight. The lines KN07 and KN10 were recommended for further breeding due to their stability and highest average weight.
Article
Biochemistry & Molecular Biology
Agnieszka Wozniak, Jacek Kesy, Paulina Glazinska, Wojciech Glinkowski, Dorota Narozna, Jan Bocianowski, Renata Rucinska-Sobkowiak, Van Chung Mai, Wlodzimierz Krzesinski, Slawomir Samardakiewicz, Beata Borowiak-Sobkowiak, Mateusz Labudda, Philippe Jeandet, Iwona Morkunas
Summary: The study aimed to understand the regulation of phytohormone biosynthesis as signaling molecules in the defense mechanisms of pea seedlings under abiotic and biotic stress factors. The expression levels of genes involved in the biosynthesis of jasmonates, ethylene, and abscisic acid were analyzed. The results showed that the expression of these genes increased in response to stress factors, particularly at sublethal doses of lead and aphid infestation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Jan Bocianowski, Agnieszka Tomkowiak, Marianna Bocianowska, Aleksandra Sobiech
Summary: Association analysis using DArTseq technology revealed 105 molecular markers associated with maize heterosis. Three markers were consistently associated with yield improvement across multiple environments.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2023)
Article
Zoology
M. Paprocka, K. Dancewicz, B. Kordan, M. Damszel, I. Sergiel, M. Biesaga, J. Mroczek, J. Bocianowski, B. Gabrys
Summary: In this study, the susceptibility of different white-berried grapevine cultivars to black bean aphid and green peach aphid was evaluated using the EPG technique. The study also analyzed the chemical composition and leaf anatomy parameters of grapevine leaves. The results showed that different cultivars had varying degrees of susceptibility to aphids, which may be related to the chemical composition of the leaves. The study also revealed that while the direct damage caused by aphids to grapevines is low, they may pose a serious threat as vectors of viruses.
EUROPEAN ZOOLOGICAL JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Aleksandra Sobiech, Agnieszka Tomkowiak, Jan Bocianowski, Grazyna Szymanska, Bartosz Nowak, Maciej Lenort
Summary: This study aims to identify new markers associated with resistance to fusarium head blight in maize plants using next-generation sequencing, association and physical mapping. Two markers have been found to effectively differentiate between susceptible and resistant genotypes, providing potential tools for selecting fusarium-resistant genotypes in maize.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Alireza Pour-Aboughadareh, Shirali Koohkan, Hassan Zali, Akbar Marzooghian, Ahmad Gholipour, Masoome Kheirgo, Ali Barati, Jan Bocianowski, Alireza Askari-Kelestani
Summary: One of the most important effects of climatic changes is the increase in temperature and water deficit stress in tropical and subtropical regions. This study investigated the performance of 56 barley genotypes in warm climate of Iran to identify high-yielding and adapted genotypes. The analysis of variance and various statistical models revealed the significant effects of genotypes, environments, and their interaction. Several selection indices and BLUP-based indices were used to identify the most desirable and superior genotypes. Overall, genotypes G24, G29, and G57 were found to be well-adapted to the warm regions of Iran.