期刊
JOURNAL OF PHYSICAL CHEMISTRY B
卷 121, 期 49, 页码 11124-11131出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jpcb.7b09173
关键词
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资金
- Japan Society for the Promotion of Science KAKENHI [24224012, 16H06024, 15H02185]
- Ministry of Education, Culture, Sports, Science and Technology KAKENHI [25108002]
- Collaborative Research Program of Institute for Chemical Research, Kyoto University [2016-76, 2017-87]
- Grants-in-Aid for Scientific Research [25108002, 26810075, 15K12188, 16H06024, 17K18795, 17H06087, 15H02185, 17K14502, 25108001] Funding Source: KAKEN
The outermost surface of the leaves of land plants is covered with a lipid membrane called the cuticle that protects against various stress factors. Probing the molecular-level structure of the intact cuticle is highly desirable for understanding its multifunctional properties. We report the in situ characterization of the surface structure of Kalanchoe pinnata leaves using polarization-modulation infrared reflection absorption spectroscopy (PM-IRRAS). Without sample pretreatment, PM-IRRAS measures the IR spectra of the leaf cuticle of a potted K. pinnata plant. The peak position of the CH2-related modes shows that the cuticular waxes on the leaf surface are mainly crystalline, and the alkyl chains are highly packed in an all-trans zigzag conformation. The surface selection rule of PM-IRRAS revealed the average orientation of the cuticular molecules, as indicated by the positive and negative signals of the IR peaks. This unique property of PM-IRRAS revealed that the alkyl chains of the waxes and the main chains of polysaccharides are oriented almost perpendicular to the leaf surface. The nondestructive, background-free, and environmental gas-free nature of PM-IRRAS allows the structure and chemistry of the leaf cuticle to be studied directly in its native environment.
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