期刊
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
卷 136, 期 -, 页码 55-65出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jpba.2016.12.036
关键词
Size exclusion chromatography; Phosphorothioate oligonucleotides; Nucleic acids therapeutics
We evaluated size exclusion chromatography (SEC) for the detection of high-order structure of phosphorothioate oligonucleotides (PS-oligo). Because of strong interaction between PS-oligo and column packing material, peaks were broader and elution time was longer than those of the corresponding natural DNA oligonucleotides. However, single- and double-stranded structures of PS-oligo were clearly separated and discriminated, while single-stranded with high-order structures such as G-quadruplex and hairpin structure were not distinguished from each other. (C) 2016 Elsevier B.V. All rights reserved.
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