期刊
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
卷 145, 期 -, 页码 195-202出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jpba.2017.06.039
关键词
Anemarrhenae Rhizome; Fibrous root; alpha-glucosidase inhibition; HPLC-QQQ/MS
资金
- National Natural Science Foundation of China [81322051]
- Project Funded by the Priority Academic Program Development(PAPD) of Jiangsu Higher Education Institutions
Comprehensive utilization of medicinal plant resources is of great significance for sustainable development of traditional Chinese medicines. In the present study, the alpha-glucosidase inhibitory activities of the rhizome and fibrous root ofAnemarrhenaAsphodeloides Bunge, were compared detailedly, and a high performance liquid chromatography coupled with electrospray ionization tandem triple quadrupole mass spectrometry (HPLC-QQQ/MS) method was developed for simultaneous quantification of seven bioactive constituents including neomangiferin, mangiferin, isomangiferin, timosaponin BII, timosaponin B, timosaponin AIII, and timosaponin N in 40 batches of samples. The results demonstrated that fibrous root extracts had more potent alpha-glucosidase inhibitory activity than rhizome extracts. Mangiferin and isomangiferin were abundant in fibrous root, while the analyzed saponins were rich in rhizome. Based on the chemometrics methods including principal component analysis (PCA), orthogonal partial least square discriminant analysis (OPLS-DA), and partial least square (PLS), mangiferin and isomangiferin might be mainly responsible for alpha-glucosidase inhibitory activity of the genus. These findings indicate that the established HPLC-QQQ/MS method was proven to be useful and efficient for quality control of Anemarrhena materials, and fibrous root had the potential to be utilized as anti -diabetic medicinal resource. (C) 2017 Elsevier B.V. All rights reserved.
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