Article
Microbiology
Weimin Wan, Xiaochun Yang, Hua Yu, Min Wang, Wei Jia, Bin Huang, Fen Qu, Bin Shan, Yi-Wei Tang, Liang Chen, Hong Du
Summary: This multi-center study in China revealed the prevalence and antimicrobial resistance characteristics of carbapenem-resistant Klebsiella oxytoca complex, showing a polyclonal dissemination pattern.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Pharmacology & Pharmacy
Beata Weber-Dabrowska, Maciej Zaczek, Malgorzata Lobocka, Marzanna Lusiak-Szelachowska, Barbara Owczarek, Filip Orwat, Norbert Lodej, Aneta Skaradzinska, Lukasz Laczmanski, Dariusz Martynowski, Marta Kaszowska, Andrzej Gorski
Summary: In recent years, multidrug-resistant strains of Klebsiella pneumoniae have become a global concern and bacteriophage therapy may provide a potential solution. This study evaluated the properties and effectiveness of bacteriophages against Klebsiella strains, identifying promising candidates for therapeutic purposes. Additionally, historical phages were examined, including one with lytic activity that has been maintained for nearly 60 years.
Article
Environmental Sciences
Tao Zhang, Shuang-yan Xu, Hui Lin, Jian Yang, Zhuo-qun Zhao, Damia Barcelo, Hua-bao Zheng
Summary: In this study, a new tylosin-degrading strain, TYL-T1, was isolated and its degradation mechanism was revealed through transcriptome and metabolite analysis. The results demonstrate the potential of TYL-T1 in removing tylosin from the environment.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Microbiology
Tufail Bashir, Rowan D. Brackston, Christopher Waite, Ioly Kotta-Loizou, Matthew R. Carey, Christoph Engl, Martin Buck, Jorg Schumacher
Summary: This study investigates the molecular origins of phenotypic heterogeneity in clonal bacterial batch cultures under extreme low-nitrogen stress. The researchers found high heterogeneity of nifHDK gene expression, which codes for the structural genes of nitrogenase, in the model diazotroph Klebsiella oxytoca. Through analysis and experiments, they identified the likely molecular origins of this heterogeneity and discovered the significant effect of the regulatory gene ginK on nifHDK variance. This understanding can inform biotechnology strategies and potentially have implications for nitrogen fixation in natural soil environments.
Article
Microbiology
Nagender Ledala, Mishika Malik, Karim Rezaul, Sara Paveglio, Anthony Provatas, Aaron Kiel, Melissa Caimano, Yanjiao Zhou, Jonathan Lindgren, Kristyna Krasulova, Peter Illes, Zdenek Dvorak, Sandhya Kortagere, Sabine Kienesberger, Amar Cosic, Lisa Poeltl, Ellen L. Zechner, Subho Ghosh, Sridhar Mani, Justin D. Radolf, Adam P. Matson
Summary: In this study, it was shown that bacterial indole acts as a multifunctional mitigator of pathogenicity by suppressing toxin production, enhancing conversion of tilimycin to tilivalline, and activating PXR.
Review
Microbiology
Nakul Neog, Upasana Phukan, Minakshi Puzari, Mohan Sharma, Pankaj Chetia
Summary: Klebsiella oxytoca is emerging as an important opportunistic pathogen causing hospital infections, especially posing a high risk for immunocompromised patients. It can lead to a variety of diseases, including colitis and infective endocarditis, in addition to common urinary and respiratory tract infections. The bacterium exhibits resistance to a wide range of antibiotics.
CURRENT MICROBIOLOGY
(2021)
Article
Microbiology
Christopher J. Waite, Anya Lindstrom Battle, Mark H. Bennett, Matthew R. Carey, Chun K. Hong, Ioly Kotta-Loizou, Martin Buck, Jorg Schumacher
Summary: Free-living nitrogen-fixing bacteria can improve growth yields of non-leguminous plants and address nutrient imbalances in crop production. Nitrogen fixation is a resource-costly adaptation regulated by signaling processes, and reinvestment of fixed nitrogen is essential for sustained nitrogenase activity. The study highlights the quantitative resource allocation required for successful nitrogen fixation in free-living diazotrophs.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Deyan Donchev, Ivan N. N. Ivanov, Ivan Stoikov, Stefana Sabtcheva, Yordan Kalchev, Marianna Murdjeva, Elina Dobreva, Rumyana Hristova
Summary: The study improved and extended the multi-locus VNTR analysis (MLVA) for strain-level differentiation of Klebsiella pneumoniae species complex. The modified scheme (MLVA8+) showed high concordance with classic MLST and provided greater differentiation capability. MLVA8+ also demonstrated its applicability in investigating the intra-hospital spread of carbapenem-resistant producers. It offers improved typing accuracy and extended typing scope for Klebsiella pneumoniae.
Review
Microbiology
Jing Yang, Haiyan Long, Ya Hu, Yu Feng, Alan McNally, Zhiyong Zong
Summary: Klebsiella oxytoca is a complex of nine species that can cause various infections and is increasingly becoming resistant to antibiotics, posing a potential threat to human health.
CLINICAL MICROBIOLOGY REVIEWS
(2022)
Article
Pediatrics
Michelle Bous, Malte Tielsch, Cihan Papan, Elisabeth Kaiser, Regine Weber, Joerg Ingo Baumbach, Soeren L. Becker, Michael Zemlin, Sybelle Goedicke-Fritz
Summary: The study developed a method for analyzing volatile organic compound (VOC) profiles of different bacterial species using MCC/IMS. The MCC/IMS analysis showed clear differentiation between Klebsiella pneumoniae (KP) and Klebsiella oxytoca (KO), as well as high sensitivity, specificity, and positive predictive values for identifying these bacteria. The results suggest that MCC/IMS may be a useful tool for in vitro diagnostics.
FRONTIERS IN PEDIATRICS
(2023)
Article
Infectious Diseases
Eva Leitner, Michael Bozic, Sabine Kienesberger, Amar Cosic, Olfert Landt, Christoph Hoegenauer, Harald H. Kessler
Summary: This study evaluated a new test kit for detecting toxin genes of Klebsiella oxytoca, which causes antibiotic-associated diarrhea. The results showed that the test kit has high sensitivity and can be used as the preferred method for improving the diagnosis of AAHC.
CLINICAL MICROBIOLOGY AND INFECTION
(2022)
Article
Microbiology
Haiyan Long, Ya Hu, Yu Feng, Zhiyong Zong
Summary: The Klebsiella oxytoca complex consists of nine closely related species that cause human infections, with K. oxytoca and Klebsiella michiganensis being more commonly associated with human infections and carrying more virulence genes.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Veterinary Sciences
Takeshi Tsuka, Hiroichi Ozaki, Daisuke Saito, Toshiyuki Murase, Yoshiharu Okamoto, Kazuo Azuma, Tomohiro Osaki, Norihiko Ito, Yusuke Murahata, Tomohiro Imagawa
Summary: CTX-M-2-producing Klebsiella oxytoca strains with high ESBL production rates may spread clonally within a farm, while the bla(CTX-M-2) gene in Klebsiella pneumoniae may disseminate among farms through different plasmids. Monitoring ESBL genes, including bla(CTX-M-2), among causative agents of bacterial mastitis in cows can help develop relevant treatments and control practices.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Jiaxue Zhao, Yanyan Xi, Jiangfeng Zhang, Yuefei Jin, Haiyan Yang, Guangcai Duan, Shuaiyin Chen, Jinzhao Long
Summary: This study investigated the prevalence and diversity of CRISPR/Cas systems in 175 Klebsiella oxytoca strains. It was found that 58.86% of the strains possessed at least one confirmed CRISPR locus, with the I-F system being the most prevalent. The IV-A3 system was randomly distributed and could be divided into two subgroups. Space origin analysis revealed significant matches between some spacer sequences and mobile genetic elements. The phage tail tape measure protein and conjunctive transfer system protein were identified as important targets of the I-F and IV-A3 systems, respectively. The predicted PAM sequences for the different systems were also identified.
MOLECULAR GENETICS AND GENOMICS
(2023)
Article
Engineering, Environmental
Shanshan Lin, Yinghao Song, Yang Huo, Qi Wang, Xiangru Liu, Ya Gao, Wei Fan, Mingxin Huo
Summary: This study investigated the copper transport and trafficking mechanisms using the Cu resistant strain Klebsiella oxytoca CAV 1374, and found that the strain is a suitable biosorbent for copper removal. Different uptake mechanisms and pathways were identified under different copper concentrations, providing critical insights into copper-uptake mechanisms of microorganisms.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Gastroenterology & Hepatology
Theresa M. Greimel, Laura Stampfer, Eva Leitner, Sabine Kienesberger, Ellen L. Zechner, Michael Bozic, Gabriel E. Wagner, Katrin Unterhauser, Maksym Kitsera, Almuthe C. Hauer, Gregor Gorkiewicz, Philipp Wurm, Francesco Valitutti, Christoph Hogenauer, Karl Martin Hoffmann
Summary: Over 70% of healthy infants were found to be colonized with K oxytoca, but toxin levels were below detection level and only half of the isolates produced toxin in vitro. This has important implications for future disease association studies.
JOURNAL OF PEDIATRIC GASTROENTEROLOGY AND NUTRITION
(2022)
Article
Microbiology
Nagender Ledala, Mishika Malik, Karim Rezaul, Sara Paveglio, Anthony Provatas, Aaron Kiel, Melissa Caimano, Yanjiao Zhou, Jonathan Lindgren, Kristyna Krasulova, Peter Illes, Zdenek Dvorak, Sandhya Kortagere, Sabine Kienesberger, Amar Cosic, Lisa Poeltl, Ellen L. Zechner, Subho Ghosh, Sridhar Mani, Justin D. Radolf, Adam P. Matson
Summary: In this study, it was shown that bacterial indole acts as a multifunctional mitigator of pathogenicity by suppressing toxin production, enhancing conversion of tilimycin to tilivalline, and activating PXR.
Article
Medicine, General & Internal
Francesco Fortarezza, Federica Pezzuto, Paul Hofman, Izidor Kern, Angel Panizo, Jan von der Thuesen, Sergei Timofeev, Gregor Gorkiewicz, Sabina Berezowska, Laurence de Leval, Cristian Ortiz-Villalon, Francesca Lunardi, Fiorella Calabrese
Summary: Autoptic studies of patients who died from COVID-19 are crucial for improving our understanding of the disease and its impact on patient management and treatment.
Article
Hematology
Francesco Marchesi, Jon Salmanton-Garcia, Ziad Emarah, Klara Piukovics, Marcio Nucci, Alberto Lopez-Garcia, Zdenek Racil, Francesca Farina, Marina Popova, Sofia Zompi, Ernesta Audisio, Marie-Pierre Ledoux, Luisa Verga, Barbora Weinbergerova, Tomas Szotkovski, Maria Gomes Da Silva, Nicola Fracchiolla, Nick De Jonge, Graham Collins, Monia Marchetti, Gabriele Magliano, Carolina Garcfa-Vidal, Monika M. Biernat, Jaap Van Doesum, Marina Machado, Fatih Demirkan, Murtadha Al-Khabori, Pavel Zak, Benjamfn Vfsek, Igor Stoma, Gustavo-Adolfo Mendez, Johan Maertens, Nina Khanna, Ildefonso Espigado, Giulia Dragonetti, Luana Fianchi, Maria Ilaria Del Principe, Alba Cabirta, Irati Ormazabal-Velez, Ozren Jaksic, Caterina Buquicchio, Valentina Bonuomo, Josip Batinic, Ali S. Omrani, Sylvain Lamure, Olimpia Finizio, Noemf Fernandez, Iker Falces-Romero, Ola Blennow, Rui Bergantim, Natasha Ali, Sein Win, Jens Van Praet, Maria Chiara Tisi, Ayten Shirinova, Martin Schoenlein, Juergen Prattes, Monica Piedimonte, Verena Petzer, Milan Navratil, Austin Kulasekararaj, Pavel Jindra, Jiff Sramek, Andreas Glenthoj, Rita Fazzi, Cristina De Ramon-Sanchez, Chiara Cattaneo, Maria Calbacho, Nathan C. Bahr, Shaimaa El-Ashwah, Raul Cordoba, Michaela Hanakova, Giovanni Paolo Maria Zambrotta, Giovanni Zambrotta, Mariarita Sciume, Stephen Booth, Raquel Nunes Rodrigues, Maria Vittoria Sacchi, Nicole Garcia-Pouton, Juan-Alberto Martin-Gonzalez, Sofya Khostelidi, Stefanie Graefe, Laman Rahimli, Emanuele Ammatuna, Alessandro Busca, Paolo Corradini, Martin Hoenigl, Nikolai Klimko, Philipp Koehler, Antonio Pagliuca, Francesco Passamonti, Oliver A. Cornely, Livio Pagano
Summary: Patients with acute myeloid leukemia (AML) who are diagnosed with COVID-19 are at high risk of death. The management of AML patients with COVID-19 has not been established. In our study, a significant number of AML patients receiving or having received AML treatment had severe or critical COVID-19. Chemotherapy was modified or discontinued in a substantial portion of patients. The mortality rate was high, and survival was better when AML treatment could be delayed.
Article
Gastroenterology & Hepatology
Christoph Hoegenauer, Heinz F. Hammer, Alexander Mahnert, Christine Moissl-Eichinger
Summary: Archaea, as a part of human gut microbiota, play an important role in human health and disease. However, their significance remains largely unknown due to their non-bacterial biology and detection issues.
NATURE REVIEWS GASTROENTEROLOGY & HEPATOLOGY
(2022)
Article
Medicine, General & Internal
Maximilian Kutschera, Gottfried Novacek, Walter Reinisch, Christoph Hoegenauer, Wolfgang Petritsch, Thomas Haas, Alexander Moschen, Clemens Dejaco
Summary: This paper summarizes the mechanism, clinical development, efficacy, and safety of tofacitinib, and aims to optimize its safe and effective use in daily clinical practice.
WIENER KLINISCHE WOCHENSCHRIFT
(2022)
Article
Gastroenterology & Hepatology
Matthias Egger, Angela Horvath, Florian Prueller, Peter Fickert, Malcolm Finkelman, Lisa Kriegl, Henning Gronbaek, Holger Jon Moller, Juergen Prattes, Robert Krause, Martin Hoenigl, Vanessa Stadlbauer
Summary: Liver cirrhosis, a systemic disease causing 1 million deaths per year, is associated with fungal translocation and its effects on gut integrity, inflammation, and liver disease severity/outcome. This study found a correlation between fungal translocation and markers of gut inflammation, as well as a significant difference in mortality between patients with positive versus negative fungal translocation. Further research is needed to understand the complex interactions and potential therapeutic interventions.
LIVER INTERNATIONAL
(2023)
Article
Virology
Ellen Gelpi, Sigrid Klotz, Miriam Beyerle, Sven Wischnewski, Verena Harter, Harald Kirschner, Katharina Stolz, Christoph Reisinger, Elisabeth Lindeck-Pozza, Alexander Zoufaly, Marlene Leoni, Gregor Gorkiewicz, Martin Zacharias, Christine Haberler, Johannes Hainfellner, Adelheid Woehrer, Simon Hametner, Thomas Roetzer, Till Voigtlaender, Gerda Ricken, Verena Endmayr, Carmen Haider, Judith Ludwig, Andrea Polt, Gloria Wilk, Susanne Schmid, Irene Erben, Anita Nguyen, Susanna Lang, Ingrid Simonitsch-Klupp, Christoph Kornauth, Maja Nackenhorst, Johannes Klaeger, Renate Kain, Andreas Chott, Richard Wasicky, Robert Krause, Guenter Weiss, Judith Loeffler-Rag, Thomas Berger, Patrizia Moser, Afshin Soleiman, Martin Asslaber, Roland Sedivy, Nikolaus Klupp, Martin Klimpfinger, Daniele Risser, Herbert Budka, Lucas Schirmer, Anne-Katrin Probstel, Romana Hoeftberger
Summary: We conducted a neuropathological analysis on 32 patients who died from COVID-19 in Austria between 2020 and 2021. The results showed diffuse white matter damage and microglial activation in all cases, indicating indirect brain damage rather than direct virus-specific damage. Inflammatory changes and vascular pathologies were also observed, along with silent neurodegenerative pathologies in elderly patients. These findings align with experimental data on SARS-CoV-2-related brain damage and provide a better understanding of the mechanisms underlying neurological symptoms after COVID-19.
Article
Cell Biology
Lisa Poltl, Maksym Kitsera, Sandra Raffl, Stefan Schild, Amar Cosic, Sabine Kienesberger, Katrin Unterhauser, Georg Raber, Christian Lembacher-Fadum, Rolf Breinbauer, Gregor Gorkiewicz, Carlos Sebastian, Gerald Hoefler, Ellen L. Zechner
Summary: The DNA-alkylating metabolite tilimycin is a microbial genotoxin that causes colitis in individuals carrying til+ Klebsiella spp. Intestinal stem cells are affected by tilimycin-induced DNA damage, leading to genetic aberrations and increased disease susceptibility in the colon. The presence of tilimycin-producing Klebsiella results in higher frequencies and more mutations per individual compared to non-producing mutants.
Article
Microbiology
Matthew E. Mead, Patricia Alves L. de Castro, Jacob L. Steenwyk, Jean-Pierre Gangneux, Martin Hoenigl, Juergen Prattes, Riina Rautemaa-Richardson, Helene B. Guegan, Caroline B. Moore, Cornelia Lass-Floerl, Florian Reizine, Clara Valero, Norman J. Van Rhijn, Michael J. Bromley, Antonis H. Rokas, Gustavo H. Goldman, Sara Gago, ECMM CAPA Study Grp
Summary: Secondary infections caused by Aspergillus fumigatus are a major cause of death in severe COVID-19 patients. Genomic analysis of 11 A. fumigatus isolates from CAPA patients in Europe revealed that CAPA isolates do not cluster based on geographic origin. Phenotypically, CAPA isolates are more similar to the A1160 reference strain, but differ from the Af293 strain in infection-relevant stresses. Further research is needed to understand the molecular epidemiology of CAPA and identify genetic factors contributing to copathogenicity and antifungal resistance in COVID-19 patients.
MICROBIOLOGY SPECTRUM
(2023)
Review
Dermatology
Jon Salmanton-Garcia, Ilana Reinhold, Juergen Prattes, Nico Bekaan, Philipp Koehler, Oliver A. Cornely
Summary: The growing threat of antimicrobial resistance is a global concern, particularly in the treatment of candidemia. The current recommendation of a minimum treatment duration of 14 days for candidemia is being questioned, and further research and clinical trials are needed to establish evidence-based guidelines.
Article
Cell Biology
Daniel Podlesny, Marija Durdevic, Sudarshan Paramsothy, Nadeem O. Kaakoush, Christoph Hogenauer, Gregor Gorkiewicz, Jens Walter, W. Florian Fricke
Summary: The study reveals that the coexistence of donor and recipient strains after fecal microbiota transplantation is rare, and the engraftment of donor strains is strongly positively correlated with recipient microbiota dysbiosis. Engraftment of donor strains can be enhanced through antibiotic pretreatment and bowel lavage, and is dependent on the diversity of donor and recipient microbiota.
CELL REPORTS MEDICINE
(2022)
Meeting Abstract
Pathology
M. Zacharias, A. Thueringer, K. Kashofer, R. Krause, G. Gorkiewicz
