Article
Infectious Diseases
Helio S. Sader, Rodrigo E. Mendes, S. J. Ryan Arends, Cecilia G. Carvalhaes, Mariana Castanheira
Summary: The study evaluated the activity of aztreonam-avibactam against Enterobacterales isolates from European medical centers and the occurrence of carbapenemases. The results showed that aztreonam-avibactam had high inhibitory activity against Enterobacterales isolates and retained potent activity against resistant strains. The study also found that isolates from the Eastern European and Mediterranean region had lower susceptibility to comparator agents compared to isolates from Western Europe.
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2022)
Article
Infectious Diseases
Remy A. Bonnin, Sandrine Bernabeu, Cecile Emeraud, Elodie Creton, Oceane Vanparis, Thierry Naas, Agnes B. Jousset, Laurent Dortet
Summary: This study demonstrates that the addition of relebactam and vaborbactam did not significantly modify the susceptibility of OXA-48-like-producing Enterobacterales to imipenem and meropenem. However, the addition of avibactam strongly restored ceftazidime susceptibility.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2022)
Review
Food Science & Technology
Rafael M. P. Poeys-Carvalho, Alice G. M. Gonzalez
Summary: There is an increasing concern about the occurrence of antimicrobial-resistant Enterobacteriaceae in vegetables. Vegetables serve as vectors for the dissemination of resistance mechanisms previously observed in hospitals.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Article
Infectious Diseases
Mariana Castanheira, Timothy B. Doyle, Lalitagauri M. Deshpande, Rodrigo E. Mendes, Helio S. Sader
Summary: Research on carbapenem-resistant Enterobacterales (CRE) without carbapenemase genes revealed complex resistance mechanisms, limited activity of most beta-lactam agents, and effective inhibition by ceftazidime/avibactam. Further studies are needed to understand the diverse resistance mechanisms and optimize treatment strategies for these isolates.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2021)
Article
Infectious Diseases
Hui Zhang, Peiyao Jia, Ying Zhu, Ge Zhang, Jingjia Zhang, Wei Kang, Simeng Duan, Weijuan Zhang, Qiwen Yang, Yingchun Xu
Summary: This study investigated the susceptibility of imipenem/relebactam for infections caused by A. baumannii and P. aeruginosa in China, showing that the drug is effective in treating MDR or imipenem-non-susceptible P. aeruginosa infections but not A. baumannii infections.
INFECTION AND DRUG RESISTANCE
(2021)
Article
Infectious Diseases
Vincenzo Di Pilato, Luigi Principe, Lilia Andriani, Noemi Aiezza, Marco Coppi, Silvia Ricci, Tommaso Giani, Francesco Luzzaro, Gian Maria Rossolini
Summary: This article reports an outbreak caused by carbapenemase-producing Klebsiella pneumoniae that is resistant to ceftazidime/avibactam and variably resistant to carbapenem-based beta-lactam/beta-lactamase inhibitor combinations. The overproduction of KPC-3 was identified as the main mechanism of cross-resistance to ceftazidime/avibactam and meropenem/vaborbactam, while resistance to imipenem/relebactam was less affected.
CLINICAL MICROBIOLOGY AND INFECTION
(2023)
Article
Microbiology
G. L. Drusano, Robert A. Bonomo, Steven M. Marshall, Laura J. Rojas, Mark D. Adams, Maria F. Mojica, Barry N. Kreiswirth, Liang Chen, Nino Mtchedlidze, Meredith Bacci, Michael Vicchiarelli, Jurgen B. Bulitta, Arnold Louie
Summary: The study investigated the impact of Ceftazidime-avibactam combination on Pseudomonas aeruginosa resistance and bacterial killing rate, finding a close relationship between AVI concentration and resistance emergence, and continuous infusion of AVI effectively suppressed resistance formation.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Infectious Diseases
Krisztina M. Papp-Wallace, Melissa D. Barnes, Magdalena A. Taracila, Christopher R. Bethel, Joseph D. Rutter, Elise T. Zeiser, Katherine Young, Robert A. Bonomo
Summary: Ceftazidime-avibactam is ineffective against KPC-2 D179 variants, but imipenem-relebactam can overcome their resistance. The D179Y variant is unable to hydrolyze imipenem, while the D179N variant has a slower rate. Acyl complexes are less prevalent with the D179Y variant compared to the D179N variant. Imipenem-relebactam shows promise for treating clinical isolates harboring D179 variants of KPC-2.
Article
Microbiology
Yanfang Feng, Arend L. de Vos, Shakir Khan, Mary St John, Tayyaba Hasan
Summary: Beta-lactamase inhibition and restoration of beta-lactam susceptibility in carbapenemase-producing organisms are positively correlated and depend on the specific carbapenemase molecular identity. It is crucial to use quantitative tools to guide the choice and dosing of beta-lactam/beta-lactamase inhibitor treatments, and utilize rapid molecular diagnostics to prevent ineffective treatment due to resistance mechanisms beyond beta-lactamase.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Infectious Diseases
Pisut Pongchaikul, Paninee Mongkolsuk
Summary: Antibiotic resistance, especially beta-lactam resistance, is a significant problem worldwide. IMP-type metallo-beta-lactamase (MBL) has become a prominent enzyme in Asia, with 88 variants currently identified. IMP-1 is the most common variant. IMP-type MBLs have been detected in multiple species, with Pseudomonas aeruginosa being the most frequent carrier. The distribution of IMP-type MBLs in Asia has been observed in various countries. This study provides comprehensive insight into the global and regional prevalence of IMP-type MBLs, highlighting their resistance to carbapenems and third-generation cephalosporins.
Review
Microbiology
Olga Lomovskaya, Ruslan Tsivkovski, Dongxu Sun, Raja Reddy, Maxim Totrov, Scott Hecker, David Griffith, Jeffery Loutit, Michael Dudley
Summary: QPX7728 is an ultra-broad-spectrum beta-lactamase inhibitor with potent inhibition against a wide range of clinically important beta-lactamases. It is minimally affected by common resistance mechanisms and shows broad coverage when combined with various beta-lactam antibiotics. The oral delivery option of QPX7728 also provides potential for application in combination products.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Infectious Diseases
Mohammad Irfan, Alhomidi Almotiri, Zeyad Abdullah AlZeyadi
Summary: Hospital and municipal wastewater contribute to the spread of antibiotic-resistant bacteria and genes in the environment. This study examined the antibiotic resistance and beta-lactamase production in clinically significant Gram-negative bacteria isolated from hospital and municipal wastewater. The results showed a significant rate of bacteria that produce ESBL and carbapenemase, which can promote the spread of bacterial resistance. Identifying ESBL and carbapenemase production genes in wastewater samples and their resistance patterns can provide valuable data and guide the development of pathogen management strategies to reduce multidrug resistance.
Article
Immunology
Mariana Castanheira, John H. Kimbrough, Sean DeVries, Rodrigo E. Mendes, Helio S. Sader
Summary: The temporal and longitudinal trends of beta-lactamases and their associated susceptibility patterns were analyzed for Escherichia coli and Klebsiella pneumoniae isolates consecutively collected in 56 United States hospitals during 2016-2020. The results showed that extended-spectrum beta-lactamases (ESBLs) were detected among 88.0% of the isolates without carbapenem resistance, and bla(CTX-M-15) was the most common ESBL variant. Significant increases in ESBL rates were noted among bloodstream infection and K pneumoniae isolates due to the increase in bla(CTX-M). Carbapenem resistance and carbapenemase genes were also detected, but their prevalence remained low. Ceftazidime-avibactam and carbapenems were highly active against ESBL producers without carbapenem resistance. Ceftazidime-avibactam also showed good inhibition against carbapenem-resistant isolates. Overall, the study highlights the importance of continuous surveillance of beta-lactamase-producing isolates.
OPEN FORUM INFECTIOUS DISEASES
(2023)
Article
Microbiology
Yu-Shan Huang, Pao-Yu Chen, Pei-Chun Chou, Jann-Tay Wang
Summary: Cefiderocol and aztreonam-avibactam (ATM-AVI) both showed activity against carbapenem-resistant Gram-negative bacilli, including those that produce metallo-beta-lactamases (MBLs). However, Enterobacteriaceae isolates producing NDM carbapenemase displayed lower susceptibility to both antibiotics.
MICROBIOLOGY SPECTRUM
(2023)
Article
Biotechnology & Applied Microbiology
Filipe Pereira Matteoli, Francisnei Pedrosa-Silva, Lorena Dutra-Silva, Admir Jose Giachini
Summary: Research has revealed that Serratia marcescens can be classified into 12 lineages, with some containing a large number of environmental strains while others exclusively harbor human-associated strains with specific ecological niches. The diversity of resistant genomes in different lineages underscores the importance of surveillance programs, with whole-genome sequencing providing insights into carbapenemase-producing strains of this species.
Article
Microbiology
Krisztina M. Papp-Wallace, Adam B. Shapiro, Scott A. Becka, Elise T. Zeiser, John J. LiPuma, Douglas J. Lane, Rekha G. Panchal, John P. Mueller, John P. O'Donnell, Alita A. Miller
Summary: Burkholderia genus, including pathogens causing Melioidosis and Glanders, are hard to treat, but the combination of sulbactam-durlobactam (SUL-DUR) shows potential for treating these infections by inhibiting bacterial growth and reducing beta-lactamase activity.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Microbiology
Barbara Ghiglione, Maria Margarita Rodriguez, Florencia Brunetti, Krisztina M. Papp-Wallace, Ayumi Yoshizumi, Yoshikazu Ishii, Robert A. Bonomo, Gabriel Gutkind, Sebastian Klinke, Pablo Power
Summary: The CTX-M-151 variant, isolated from a Salmonella strain in Japan, shows a unique hydrolytic preference towards ceftriaxone and is well inhibited by clavulanic acid. Its structure reveals new features and suggests it may represent a new subgroup within the CTX-M family.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Microbiology
Malligarjunan Rajavel, Vijay Kumar, Ha Nguyen, Jacob Wyatt, Steven H. Marshall, Krisztina M. Papp-Wallace, Prasad Deshpande, Satish Bhavsar, Ravindra Yeole, Sachin Bhagwat, Mahesh Patel, Robert A. Bonomo, Focco van den Akker
Summary: The study on the inhibition of multidrug-resistant pathogens by a new compound and the resistance mechanisms of antibiotics may offer insights into designing novel antibiotics to combat resistant strains.
Article
Multidisciplinary Sciences
Jasmine Martinez, Chelsea Razo-Gutierrez, Casin Le, Robert Courville, Camila Pimentel, Christine Liu, Sammie E. Fung, Marisel R. Tuttobene, Kimberly Phan, Alejandro J. Vila, Parvin Shahrestani, Veronica Jimenez, Marcelo E. Tolmasky, Scott A. Becka, Krisztina M. Papp-Wallace, Robert A. Bonomo, Alfonso Soler-Bistue, Rodrigo Sieira, Maria Soledad Ramirez
Summary: This study investigates the genetic changes and virulence factor expression of Acinetobacter baumannii in the cerebrospinal fluid (CSF) environment, showing its adaptability and survival capabilities. While a highly virulent strain showed no transcriptome changes in the presence of CSF, a low-virulence isolate exhibited significant differences in gene expression. Exposure to CSF induced increased expression of virulence factors without promoting bacterial growth, but rather increasing the expression of genes associated with transcription, translation, and ATP synthesis.
SCIENTIFIC REPORTS
(2021)
Article
Microbiology
Alessandro Moriano, Diego O. Serra, Amparo Hoard, Sabrina Montana, Jose Degrossi, Robert A. Bonomo, Krisztina M. Papp-Wallace, Maria Soledad Ramirez
Summary: Polymicrobial lung infections in individuals with Cystic Fibrosis are complex and can be caused by opportunistic pathogens like S. aureus, P. aeruginosa and Burkholderia cepacia complex. Interaction studies between S. aureus and Bcc reveal potential impact on the severity and outcomes of infections in CF patients.
CURRENT MICROBIOLOGY
(2021)
Article
Chemistry, Medicinal
Michiyoshi Nukaga, Michael J. Yoon, Magdalena A. Taracilia, Tyuji Hoshino, Scott A. Becka, Elise T. Zeiser, Joseph R. Johnson, Krisztina M. Papp-Wallace
Summary: The Burkholderia cepacia complex (Bcc) poses a serious health threat to individuals with cystic fibrosis or compromised immune systems due to its high level of antibiotic resistance, notably through the PenA-like beta-lactamases. This study evaluated different beta-lactamase inhibitors against PenA1 to restore susceptibility to antibiotics in whole cell based assays, revealing avibactam as the most effective inhibitor. Specific amino acids, such as S130, R220, and K234, were found to play important roles in inactivation by avibactam, indicating potential targets for future drug development.
ACS INFECTIOUS DISEASES
(2021)
Article
Microbiology
Camila Pimentel, Casin Le, Marisel R. Tuttobene, Tomas Subils, Jasmine Martinez, Rodrigo Sieira, Krisztina M. Papp-Wallace, Niroshika Keppetipola, Robert A. Bonomo, Luis A. Actis, Marcelo E. Tolmasky, Maria Soledad Ramirez
Summary: Acinetobacter baumannii, a nosocomial pathogen with high antibiotic resistance, has been shown to adapt to different host environments, providing insights into potential novel antimicrobial strategies. Research on the gene expression profile of A. baumannii when exposed to human fluids revealed key pathways involved in the pathogen's response.
Article
Microbiology
Casin Le, Camila Pimentel, Marisel R. Tuttobene, Tomas Subils, Krisztina M. Papp-Wallace, Robert A. Bonomo, Luis A. Actis, Marcelo E. Tolmasky, Maria Soledad Ramirez
Summary: Acinetobacter baumannii, a multidrug-resistant pathogen, is affected by exposure to human body fluids, leading to changes in gene expression and pathogenic behavior, with different strains showing varying degrees of susceptibility. Human serum albumin plays a crucial role in HPF, modulating gene expression and behavior in A. baumannii.
CURRENT MICROBIOLOGY
(2021)
Article
Chemistry, Medicinal
Joel A. Goldberg, Vijay Kumar, Elizabeth J. Spencer, Denton Hoyer, Steven H. Marshall, Andrea M. Hujer, Kristine M. Hujer, Christopher R. Bethel, Krisztina M. Papp-Wallace, Federico Perez, Michael R. Jacobs, David van Duin, Barry N. Kreiswirth, Focco van den Akker, Mark S. Plummer, Robert A. Bonomo
Summary: This study demonstrates the preclinical evaluation of a novel γ-lactam siderophore antibiotic, YU253911, showing potent antimicrobial activity against multidrug-resistant Gram-negative pathogens. The compound targets penicillin-binding protein 3 in Pseudomonas aeruginosa and has dose-dependent efficacy in a mouse infection model. Additionally, a combination with a novel beta-lactamase inhibitor was shown to enhance the effectiveness of the antibiotic against carbapenem-resistant Acinetobacter spp.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Infectious Diseases
Camila Pimentel, Casin Le, Marisel R. Tuttobene, Tomas Subils, Krisztina M. Papp-Wallace, Robert A. Bonomo, Marcelo E. Tolmasky, Maria Soledad Ramirez
Summary: The study exposed different Acinetobacter baumannii strains to human serum albumin (HSA) and human serum (HS) to evaluate their response in terms of antimicrobial resistance, biofilm formation, and quorum sensing. The results showed varying gene expression levels and responses among different strains when exposed to HSA and HS, indicating different adaptative responses in infecting A. baumannii strains.
Editorial Material
Microbiology
Krisztina M. Papp-Wallace, Jean-Denis Docquier, Frederic Kerff, Pablo Power
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
T. A. Alsenani, S. L. Viviani, V Kumar, M. A. Taracila, C. R. Bethel, M. D. Barnes, K. M. Papp-Wallace, R. K. Shields, M. H. Nguyen, C. J. Clancy, R. A. Bonomo, F. van den Akker
Summary: The structural study of KPC-2 D179 variants revealed destabilization of the Omega loop, contributing to ceftazidime-avibactam resistance.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Microbiology
Magdalena A. Taracila, Christopher R. Bethel, Andrea M. Hujer, Krisztina M. Papp-Wallace, Melissa D. Barnes, Joseph D. Rutter, Jamie VanPelt, Ben A. Shurina, Focco van den Akker, Cornelius J. Clancy, M. Hong Nguyen, Shaoji Cheng, Ryan K. Shields, Richard C. Page, Robert A. Bonomo
Summary: This study investigates the susceptibility and resistance mechanism of D179Y variant of KPC-2 and -3 to carbapenems and ceftazidime and ceftazidime-avibactam, and it reveals that conformational changes and overall disorder caused by D179Y substitution contribute to the unexpected phenotype.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Microbiology
Philip Hinchliffe, Catherine L. Tooke, Christopher R. Bethel, Benlian Wang, Christopher Arthur, Kate J. Heesom, Stuart Shapiro, Daniela M. Schlatzer, Krisztina M. Papp-Wallace, Robert A. Bonomo, James Spencer
Summary: This study reveals the mechanism of action of two β-lactamase inhibitors, enmetazobactam and tazobactam, on CTX-M-15 β-lactamase in Escherichia coli, including the formation of protein cross-link and epimerization of amino acids.
Article
Infectious Diseases
Scott A. Becka, Elise T. Zeiser, John J. LiPuma, Krisztina M. Papp-Wallace
Summary: This study evaluated the antimicrobial activity of Tebipenem against multiple strains of Enterobacterales pathogens. The results showed that all strains of Burkholderia cepacia complex (Bcc) and Burkholderia gladioli tested were resistant to Tebipenem. Further experiments revealed that the resistance may be related to the inducible beta-lactamases PenA and AmpC produced by these strains.
Article
Infectious Diseases
Syed Iqbal Haider, Naeem Akhtar, Muhammad Saleem, Sheraz Ahmed, Shiza Nadeem, Maham Amjad, Faiz Ul Haq
Summary: This study aimed to compare the diagnostic potential of Raman spectroscopy with rapid urease test and histopathology in diagnosing H. pylori infection. The results showed that Raman spectroscopy had higher sensitivity, accuracy, and specificity compared to the rapid urease test and histopathology. This study demonstrates the applicability of Raman spectroscopy as an innovative detection tool for molecular diagnosis of H. pylori infection in gastritis.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Editorial Material
Infectious Diseases
Arati Mane, Reshu Agarwal, Meenu Bajpai, Suvarna Sane, Pallavi Vidhate, Partha Rakshit, Preeti Madan, Hema Gogia, Priya Abraham, Sandhya Kabra, Ekta Gupta
Summary: The present study aimed to determine the diagnostic performance of dried blood spot (DBS) for HBsAg and anti-HCV detection using CLIA in three different laboratories across India. DBS can be a simple and convenient alternative to plasma or serum for HBsAg detection, but site-specific validation of the assay is necessary for anti-HCV detection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Somayeh Yazdanpanah, Maryam Rahbarmah, Marjan Motamedi, Hossein Khodadadi
Summary: This study compared the clinical characteristics of Fungitell and Dynamiker Fungus assays for diagnosing candidemia. The results showed that the DFA assay performed excellently, with high consistency with the FA assay and superior diagnostic performance.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Or Kriger, Natasha Belausov, Shiraz Gefen-Halevi, Nadezda Savieva, Sharon Amit
Summary: This study demonstrates that the usage of preservative-containing urine tubes may inhibit pathogens in low-volume pediatric urine samples, resulting in false-negative results. We advocate for large-scale validations by regulators to ensure result consistency.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Merve Zerey Albayrak, Sureyya Gul Yurtsever, Bilal Olcay Peker, Tuba Muderris, Selcuk Kaya
Summary: This study evaluated the antibody and T cell responses of homologous and heterologous booster doses for SARS-CoV-2 vaccines. The results showed that individuals who received heterologous boosters had higher antibody levels and higher levels of IFN-gamma, IL-2, and IL-13.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Sibra R. M. Shihab, Bushran N. Iqbal, Shiyamalee Arunasalam, Faseeha Noordeen
Summary: This study investigated the effect of sample-based RNA quality on COVID-19 real-time RT-PCR results. The purity of the extracts and concentration of RNA were found to impact test interpretations. The presence of impurities led to inconclusive test results.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Ellen Vancutsem, Florence Crombe, Oriane Soetens, Magali Wautier, Corinna Dordelmann, Denis Pierard, Ingrid Wybo, Thomas Demuyser
Summary: Quantification of EBV DNA is crucial in transplantation settings for post-transplantation diagnosis. This study evaluated the performance of the AltoStar (R) EBV PCR Kit 1.5 on whole blood samples and found it to be reliable and accurate, with good sensitivity and linear range for EBV viral load determination.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Nouf K. Almaghlouth, Panagiotis Arvanitis, Kendra Vieira, Abby London, Dimitrios Farmakiotis
Summary: This study evaluated the performance of the Viracor CMV-T-cell immunity Panel (TCIP) in predicting CMV events. The results showed that CMV-specific CD4+ and CD8(+)T-cells were significantly lower in those with CMV events, and the TCIP could be a useful adjunct tool in individualized management of CMV infection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Linzhen Zhu, Haoting Zhang, Haibo Gu, Jianying Zhou
Summary: This case report describes a 70-year-old male patient with previously unknown immunodeficiency. Multiple pulmonary nodular shadows were observed on radiography. Fungal infection was detected in various samples, and the patient was ultimately diagnosed with disseminated Talaromyces marneffei infection and B-cell non-Hodgkin's lymphoma. The main significance of this study is to emphasize the importance for clinicians to obtain comprehensive specimens from patients presenting with multiple masses in order to ensure accurate clinical diagnosis.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Ming Chang, Sambasivan Venkatasubramanian, Holly Barrett, Kevin B. Urdahl, Kris M. Weigel, Gerard A. Cangelosi, Javeed A. Shah, Aparajita Saha, Libing Feng, Kristin N. Adams, David R. Sherman, Nahum Smith, Chetan Seshadri, Sean C. Murphy, James G. Kublin
Summary: Efforts are being made globally to develop vaccines and drugs against M. tuberculosis. This study developed a real-time RT-PCR assay to detect pre-rRNA as a biomarker for bacterial viability. The assay showed promising results in mouse models, indicating its potential in future clinical studies for tuberculosis drugs and vaccines.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Letter
Infectious Diseases
Temi Lampejo, Fadia Alsheikh, Declan Crilly, Martin Brown
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Yao Li, Pengfei Shi, Rong Zhu
Summary: This study successfully diagnosed a case of lung abscess caused by Porphyromonas endodontalis using mNGS technology, highlighting the importance of considering inhalation diseases caused by oral pathogens in patients with periodontal diseases and respiratory symptoms.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Mariana Quaresma de Souza, Dienefer Venske Bierhals, Ana Julia Reis, Erica Chimara, Julia Silveira Vianna, Andrea von Groll, Pedro Almeida da Silva, Ivy Bastos Ramis
Summary: This study proposed and evaluated a drug susceptibility testing method using 2,3,5-triphenyl tetrazolium chloride (TTC) as a colorimetric indicator for the clinical relevant non-tuberculous mycobacteria (Mycobacterium abscessus complex (MABC), M. avium complex (MAC), and M. kansasii). The results showed high agreement between TTC assay and the recommended broth microdilution method, indicating the potential of TTC as a promising indicator in drug susceptibility testing for NTM.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Shanshan Zhang, Mingyuan Duan, Shuang Li, Jie Hou, Ting Qin, Zhanwei Teng, Jianhe Hu, Huihui Zhang, Xiaojing Xia
Summary: Rapid detection of pathogenic microorganisms is crucial for epidemiologic identification, prevention, and control in public health. PCR-based methods have limitations in resource-limited areas due to the need for precise temperature control. Recombinase polymerase amplification (RPA) is a new nucleic acid amplification technology that can amplify DNA or RNA at a constant temperature, offering simplicity, high specificity and sensitivity, and short detection time. Microfluidic technology combined with RPA enables rapid detection of pathogenic microorganisms by integrating nucleic acid extraction, amplification, and detection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Piyush Agrawal, Aditya Upadhyay, Awanish Kumar
Summary: Tuberculosis is a lethal infectious disease caused by Mycobacterium tuberculosis. During infection, the expression of host microRNAs changes, which could be potential biomarkers for early detection of tuberculosis. Further research is needed to identify potential microRNA biomarkers, but momentum is gaining in this field and microRNAs are expected to become routine approaches for reliable diagnosis and specific therapeutic interventions.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)