期刊
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
卷 82, 期 2, 页码 143-147出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.diagmicrobio.2015.03.006
关键词
Pneumocystis jirovecii pneumonia; Quantitative real-time PCR
A total of 120 bronchoalveolar lavage specimens from HIV and non-HIV immunocompromised patients, positive for Pneumocystis jirovecii by an in house real-time polymerase chain reaction (PCR), were evaluated by the Bio-Evolution Pneumocystis real-time PCR, a commercial quantitative assay. Patients were classified in 2 categories based on clinical and radiological findings: definite and unlikely Pneumocystis pneumonia (PCP). For the in house PCR, cycle threshold 34 was established as cut-off value to discriminate definite PCP from unlikely PCP with 65% and 85% of sensitivity and specificity, respectively. For the Bio-Evolution quantitative PCR, a cut-off value of 2.8 x 10(5) copies/mL was defined with 72% and 82% of sensitivity and specificity, respectively. Overlapped zones of results for definite and unlikely PCP were observed. Quantitative PCR is probably a useful tool for PCP diagnosis. However, for optimal management of PCP in non-HIV immunocompromised patients, operational thresholds should be assessed according to underlying diseases and other clinical and radiological parameters. (C) 2015 Elsevier Inc. All rights reserved.
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