期刊
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
卷 83, 期 4, 页码 349-354出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.diagmicrobio.2015.08.003
关键词
Staphylococcus; Coagulase-negative staphylococci; MALDI-TOF MS; Score values
资金
- Ministry of Science and Technology [MOST 102-2320-B-006-026-MY3]
- Multidisciplinary Center of Excellence for Clinical Trial and Research [MOHW 104-TDU-B-211-113002]
- Department of Health and Welfare, Taiwan
Staphylococcus species are important pathogens. We evaluated 2 score cutoffs (2.0 and 1.7) and the replicate number (a single or a duplicate test) on the identification of staphylococci using the Bruker matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS). A collection of 440 clinical isolates (11 species) and 144 reference strains (36 species) was evaluated. For clinical isolates using a cutoff of 2.0 and duplicate tests, the rates of species, genus, and unreliable identifications were 93.4%, 5.7%, and 0.9% respectively, while the respective values were 99.3%, 0.2%, and 0.5% when the cutoff was 1.7. The species identification rates were significantly higher (P < 0.01) when a cutoff of 1.7 or a duplicate test was used. Similar results were obtained for reference strains. In conclusion, a cutoff of 1.7 and duplicate tests are recommended for identification of staphylococci using MALDI-TOF MS. (C) 2015 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据