Article
Oncology
Yanjing Jia, Jie Zhao, Jinjie Yang, Jie Shao, Zihao Cai
Summary: This study identified miR-301 as a key regulator in breast cancer progression, accelerating malignant phenotype through the CPEB1/SIRT1/SOX2 axis.
MOLECULAR THERAPY-ONCOLYTICS
(2021)
Article
Multidisciplinary Sciences
Shin Kedashiro, Takeshi Kameyama, Kiyohito Mizutani, Yoshimi Takai
Summary: Nectin-4, upregulated in various cancer cells, interacts with ErbB2 and its splice variants, particularly p95-ErbB2, to enhance DNA synthesis in human breast cancer cells. The combination of nectin-4 and p95-ErbB2 cooperatively regulates SOX2 gene expression and cell proliferation in suspension culture, while also activating the PI3K-AKT signaling pathway. Additionally, only nectin-4 and p95-ErbB2 together activate the Hippo signaling pathway, influencing cell proliferation and gene expression.
SCIENTIFIC REPORTS
(2021)
Article
Cell Biology
Yunyun Chen, Kun Zhang, Rui Zhang, Zhuo Wang, Liang Yang, Tingting Zhao, Shihui Zhang, Yong Lin, Hongzhou Zhao, Yongpan Liu, Yuxuan Wei, Yijian Zhou, Jiaying Zhang, Xianzong Ye, Jing Zhao, Xinxin Li, Jianwen Que, Songlin Shi, Kuancan Liu
Summary: Research suggests that SOX2 functions as an oncogene in esophageal squamous cell carcinoma (ESCC). However, directly targeting SOX2 is not feasible due to its roles in tissue maintenance. This study identified CDP as a SOX2 binding partner enriched in ESCC and found that blocking the interaction between CDP and SOX2 with peptide aptamers, such as P58, inhibits ESCC progression. Synthetic peptide P58, containing a cell-penetrating peptide and a fluorophore, also demonstrated efficacy in blocking ESCC growth and metastasis in mice and zebrafish. Targeting the SOX2 binding partner CDP with peptide P58 provides an alternative approach to treating ESCC with increased SOX2 levels.
CELL DEATH DISCOVERY
(2023)
Editorial Material
Immunology
Aniqa B. Khan, Clinton S. Robbins
Summary: Mononuclear phagocyte proliferation can occur in the interstitial macrophage niche in the lung, beyond myeloid progenitor cells and mature macrophages.
Review
Endocrinology & Metabolism
Marie-Claude Hofmann, Elena McBeath
Summary: This review focuses on the activation of master regulators of the niche in Sertoli cells and their targets, as well as novel molecular mechanisms underlying the regulation of growth and differentiation factors through NOTCH signaling and other pathways.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Goutam Ghosh, Kalathil K. Kartha, Gustavo Fernandez
Summary: The study highlights the significant influence of aromatic interactions on the mechanistic pathways of peptide self-assembly. Despite the peptides having similar morphologies, the minor structural difference leads to different packing modes and self-assembly mechanisms.
CHEMICAL COMMUNICATIONS
(2021)
Article
Cell & Tissue Engineering
Miriam Pagin, Mattias Pernebrink, Simone Giubbolini, Cristiana Barone, Gaia Sambruni, Yanfen Zhu, Matteo Chiara, Sergio Ottolenghi, Giulio Pavesi, Chia-Lin Wei, Claudio Cantu, Silvia K. Nicolis
Summary: The Sox2 transcription factor is crucial for the long-term self-renewal of neural stem cells. The downstream molecule Fos plays a key role in maintaining cell proliferation, possibly through regulating the expression of Socs3. The findings suggest a network of interactions involving Sox2, Fos, and Socs3 in regulating NSC proliferation and maintenance.
Article
Biochemistry & Molecular Biology
Silvia Pietrobono, Raffaella De Paolo, Domenico Mangiameli, Andrea Marranci, Ilaria Battisti, Cinzia Franchin, Giorgio Arrigoni, Davide Melisi, Laura Poliseno, Barbara Stecca
Summary: Despite advances in BRAF kinase inhibitors (BRAFi) for BRAF-mutant melanomas, resistance development remains a major clinical problem. This study identifies p38/SOX2-mediated adaptive response mechanism to BRAFi, revealing its role in providing survival signals to melanoma cells.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Medicine, Research & Experimental
Yandan Chen, Shuyi Fang, Qingwei Ding, Rongzhen Jiang, Jiefeng He, Qin Wang, Yuting Jin, Xinxin Huang, Sheng Liu, Maegan L. Capitano, Thao Trinh, Yincheng Teng, Qingyou Meng, Jun Wan, Hal E. Broxmeyer, Bin Guo
Summary: The study found that under ex vivo culturing conditions, HSCs with low levels of mitochondrial ROS were enriched in the CD34+CD133+ADGRG1+ CB cell population. These cells showed increased mitochondrial oxidative stress in transcriptomic and metabolic profiling, along with loss of stemness.
JOURNAL OF CLINICAL INVESTIGATION
(2021)
Article
Medicine, Research & Experimental
Yihong Guan, Metis Hasipek, Dongxu Jiang, Anand D. Tiwari, Dale R. Grabowski, Simona Pagliuca, Sunisa Kongkiatkamon, Bhumika Patel, Salendra Singh, Yvonne Parker, Thomas LaFramboise, Daniel Lindner, Mikkael A. Sekeres, Omar Y. Mian, Yogen Saunthararajah, Jaroslaw P. Maciejewski, Babal K. Jha
Summary: Eltrombopag binds to the TET2 catalytic domain and inhibits its dioxygenase activity, leading to the expansion of normal hematopoietic stem cells and the prevention of neoplastic evolution in TET2 mutant malignant myeloid cells. This finding provides a scientific rationale for the treatment of myeloid malignancies associated with TET2 mutations or TET deficiency.
JOURNAL OF CLINICAL INVESTIGATION
(2022)
Review
Biochemistry & Molecular Biology
Qian Yan, Xiaona Fang, Chenxi Li, Ping Lan, Xinyuan Guan
Summary: This review summarizes the common pluripotent transcription factors and development signaling pathways shared by cancer stem cells (CSCs) and embryonic stem cells (ESCs). It also discusses the newly identified oncofetal proteins that drive the self-renewal and therapy resistance of CSCs. Additionally, the review explores how targeting oncofetal drivers through clinical implementation can facilitate the development of CSCs-directed therapy.
ESSAYS IN BIOCHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Fabiana C. Malaga Gadea, Evgenia N. Nikolova
Summary: By integrating high-resolution nuclear magnetic resonance (NMR) spectroscopy with biochemistry, researchers have gained new structural and mechanistic insights into the interaction of pluripotency pioneer transcription factors (pTFs) with nucleosomes. They found that the affinity and conformation of Sox2 for solvent-exposed nucleosome sites depend on their position and DNA sequence. The study also revealed that the structure and DNA bending of Sox2 can be stabilized by Oct4 binding, influencing nucleosome engagement and Sox2-Oct4 synergy.
JOURNAL OF MOLECULAR BIOLOGY
(2023)
Editorial Material
Biochemistry & Molecular Biology
Christopher G. Hubert, Shaun R. Stauffer, Justin D. Lathia
Summary: A new signaling network that connects epigenetic regulation to self-renewal and therapeutic resistance in cancer stem cells is revealed in this study.
Article
Multidisciplinary Sciences
Bikshapathi Jagga, Megan Edwards, Miriam Pagin, Kylie M. Wagstaff, David Aragao, Noelia Roman, Jeffrey D. Nanson, Shane R. Raidal, Nicole Dominado, Murray Stewart, David A. Jans, Gary R. Hime, Silvia K. Nicolis, Christopher F. Basler, Jade K. Forwood
Summary: SOX transcription factors play critical roles in development and cell differentiation, with their nuclear localization mediated by two signals that form a binding interface on importin-alpha 3. This interface is crucial for neural stem cell maintenance and Drosophila development, providing insight into how mutations and modifications may impact nuclear localization and clinical disease.
NATURE COMMUNICATIONS
(2021)
Article
Chemistry, Applied
Yakun Li, Jianjun Liao, Haiqi Zhang, Xingsan Tang, Shiqiang Zhong, Youguo Yu, Qiuxia Li
Summary: The study developed 2D ultrathin carbon nitride/acrylate boron silane polymers by incorporating C3N4 into self-polishing acrylate boron silane polymers, enhancing the coatings' antifouling performance. The coatings showed resistance to diatoms, Staphylococcus aureus, and Escherichia coli in visible light, with weak antibacterial properties in the dark. The addition of C3N4 transformed the CNPs into environmental coatings as indicated by chemical oxygen demand values.
JOURNAL OF COATINGS TECHNOLOGY AND RESEARCH
(2021)
Editorial Material
Biochemistry & Molecular Biology
Le Tran Phuc Khoa, Yali Dou
JOURNAL OF BIOLOGICAL CHEMISTRY
(2017)
Article
Biology
Andrea Corsinotti, Frederick C. K. Wong, Tulin Tatar, Iwona Szczerbinska, Florian Halbritter, Douglas Colby, Sabine Gogolok, Raphael Pantier, Kristen Liggat, Elham S. Mirfazeli, Elis Hall-Ponsele, Nicholas P. Mullin, Valerie Wilson, Ian Chambers
Article
Cell & Tissue Engineering
Dario Acampora, Luca Giovanni Di Giovannantonio, Arcomaria Garofalo, Vincenzo Nigro, Daniela Omodei, Alessia Lombardi, Jingchao Zhang, Ian Chambers, Antonio Simeone
Article
Biochemistry & Molecular Biology
Tapan Kumar Mistri, Wibowo Arindrarto, Wei Ping Ng, Choayang Wang, Leng Hiong Lim, Lili Sun, Ian Chambers, Thorsten Wohland, Paul Robson
BIOCHEMICAL JOURNAL
(2018)
Article
Cell Biology
Man Zhang, Harry G. Leitch, Walfred W. C. Tang, Nicola Festuccia, Elisa Hall-Ponsele, Jennifer Nichols, M. Azim Surani, Austin Smith, Ian Chambers
Article
Oncology
Yuqing Sun, Bo Zhou, Fengbiao Mao, Jing Xu, Hongzhi Miao, Zhenhua Zou, Le Tran Phuc Khoa, Younghoon Jang, Sheng Cai, Matthew Witkin, Richard Koche, Kai Ge, Gregory R. Dressler, Ross L. Levine, Scott A. Armstrong, Yali Dou, Jay L. Hess
Article
Biochemistry & Molecular Biology
Nicola Festuccia, Florian Halbritter, Andrea Corsinotti, Alessia Gagliardi, Douglas Colby, Simon R. Tomlinson, Ian Chambers
Article
Multidisciplinary Sciences
Jingchao Zhang, Man Zhang, Dario Acampora, Matus Vojtek, Detian Yuan, Antonio Simeone, Ian Chambers
Article
Cell Biology
Hui Zhang, Le Tran Phuc Khoa, Fengbiao Mao, Hanshi Xu, Bo Zhou, Yu Han, Monique O'Leary, Asma Nusrat, Li Wang, Thomas L. Saunders, Yali Dou
Article
Cell & Tissue Engineering
Le Tran Phuc Khoa, Yao-Chang Tsan, Fengbiao Mao, Daniel M. Kremer, Peter Sajjakulnukit, Li Zhang, Bo Zhou, Xin Tong, Natarajan Bhanu, Chunaram Choudhary, Benjamin A. Garcia, Lei Yin, Gary D. Smith, Thomas L. Saunders, Stephanie L. Bielas, Costas A. Lyssiotis, Yali Dou
Article
Biochemistry & Molecular Biology
Nicholas P. Mullin, Joby Varghese, Douglas Colby, Julia M. Richardson, Greg M. Findlay, Ian Chambers
Summary: The self-renewal efficiency of mouse embryonic stem cells is controlled by the concentration of the transcription factor NANOG, which is phosphorylated by casein kinase I at specific residues. Phosphomimetic substitutions at certain phosphorylation sites within NANOG have site-specific functional effects, influencing DNA binding and LIF-independent self-renewal. Modeling the DNA-homeodomain complex helps explain the diverse effects of these substitutions, showing how phosphorylation may impact NANOG interactions crucial for ESC self-renewal.
Article
Biochemical Research Methods
Le Tran Phuc Khoa, Yali Dou
Article
Biochemistry & Molecular Biology
Ankita Chadda, Alexander G. Kozlov, Binh Nguyen, Timothy M. Lohman, Eric A. Galburt
Summary: In this study, it was found that the DNA damage response in Mycobacterium tuberculosis differs from well-studied model bacteria. The DNA repair helicase UvrD1 in Mtb is activated through a redox-dependent process and is closely associated with the homo-dimeric Ku protein. Additionally, Ku protein is shown to stimulate the helicase activity of UvrD1.
JOURNAL OF MOLECULAR BIOLOGY
(2024)