Article
Biotechnology & Applied Microbiology
Hongyi Liu, Shaoqing Wang, Bo Lang, Yaqian Li, Xinhua Wang, Jie Chen
Summary: In this study, it was found that the engineered Trichoderma afroharzianum strain OE:SCf, which expresses the fused Sm1-Chit42 protein, exhibited synergistic inhibition against Botrytis cinerea growth and reproduction. The SCf protein showed inhibitory effects at multiple stages of the mycoparasitic interaction between T. afroharzianum and B. cinerea, including chemotropism sensing, hyphal coiling, hydrophobicity modulation, cell wall adhesion, virulence reduction, and pathogen killing by ROS. These findings highlight a novel mycoparasitic system in Trichoderma strains engineered with chimeric proteins to combat B. cinerea, providing a solid foundation for future development of engineered Trichoderma biofungicides created with chimeric proteins.
MICROBIAL CELL FACTORIES
(2023)
Article
Biotechnology & Applied Microbiology
Lijiahong Geng, Yalun Fu, Xiaohua Peng, Zhengkun Yang, Mengke Zhang, Zhiyu Song, Ning Guo, Shuangchen Chen, Junliang Chen, Bing Bai, Airong Liu, Golam Jalal Ahammed
Summary: This study investigated the effects of Trichoderma harzianum on Botrytis cinerea and its underlying mechanisms. The results showed that Trichoderma harzianum enhanced tomato resistance to gray mold disease by regulating cellular redox, secondary metabolites, and stomatal movement.
BIOLOGICAL CONTROL
(2022)
Article
Biochemistry & Molecular Biology
Consuelo Olivares-Yanez, Evelyn Sanchez, Gabriel Perez-Lara, Aldo Seguel, Pamela Y. Camejo, Luis F. Larrondo, Elena A. Vidal, Paulo Canessa
Summary: By generating manually curated transcription factor datasets and conducting network analysis, we studied the transcriptional gene regulatory networks of Botrytis and Trichoderma, revealing significant differences in their transcriptomes under different biological paradigms and distinctive gene expression changes due to confrontation.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2021)
Article
Plant Sciences
Anna Maria Aprile, Mariangela Coppola, David Turra, Stefania Vitale, Pasquale Cascone, Gianfranco Diretto, Alessia Fiore, Valeria Castaldi, Alessandra Romanelli, Concetta Avitabile, Emilio Guerrieri, Sheridan Lois Woo, Rosa Rao
Summary: Trichoderma spp. are widely used beneficial fungi in agriculture, triggering plant resistance responses. Combining them with plant-derived compounds can enhance their effectiveness in plant defense.
JOURNAL OF PLANT INTERACTIONS
(2022)
Article
Microbiology
Gabriel Perez-Lara, Tomas C. Moyano, Andrea Vega, Luis F. Larrondo, Ruben Polanco, Jose M. Alvarez, Daniel Aguayo, Paulo Canessa
Summary: To comprehensively analyze gene expression in the phytopathogenic fungus Botrytis cinerea, a web-based gene expression browser called BEB was developed. This computationally inexpensive application provides publication-ready heatmaps of transcript levels and allows gene expression clustering and visualization options, along with the ability to download gene expression values for further exploration. The implementation of BEB is transferable to other organisms and allows for comparative transcriptomics and candidate gene identification.
Review
Biotechnology & Applied Microbiology
Xiaoqing Wu, Yuping Lyu, He Ren, Fangyuan Zhou, Xinjian Zhang, Xiaoyan Zhao, Guangzhi Zhang, Hetong Yang
Summary: This study investigated the relationship between the elimination of oxalic acid (OA) by Trichoderma afroharzianum and its antagonistic effects on Botrytis cinerea. The results showed a correlation between OA degradation and mycoparasitism in T. afroharzianum, providing a new perspective on its antagonism against B. cinerea. The study also provided theoretical data for the scientific application of T. afroharzianum in biocontrol.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Article
Microbiology
Annette Pfordt, Peet Gaumann, Andreas von Tiedemann
Summary: Species of Trichoderma are widely distributed in soils, plant roots, and decaying plant residues. Trichoderma afroharzianum has been reported as a pathogen causing ear rot disease on maize, and it may also infect wheat and barley, resulting in disease symptoms and yield losses.
Article
Forestry
Jun Sun, Yue Zhang, Zeyang Zheng, Jiachen Hao, Lijing Chen
Summary: Botrytis cinerea is a pathogenic fungus that causes necrosis in plants and is highly destructive to hazelnuts. The plant's defense mechanism against this fungus involves complex changes at molecular, biochemical, and physiological levels. A comparative transcriptome profiling study revealed moderate changes in the defense strategies of resistant and susceptible hazelnut varieties after three days of B. cinerea infection. The study identified differentially expressed disease-resistant genes in the resistant variety, suggesting their crucial role in inducing innate resistance.
Article
Agriculture, Multidisciplinary
Wenyu Han, Zhongshou Wu, Zhenhui Zhong, Jason Williams, Steven E. Jacobsen, Zuodong Sun, Yi Tang
Summary: A comprehensive analysis of natural product biosynthetic gene clusters (BGCs) from the biocontrol fungus ThT22 revealed its versatile biosynthetic capacity. The biosynthesis pathway of a previously unknown compound eujavanicol A was identified through heterologous expression. The activation of silent BGCs in ThT22 was also demonstrated through transcriptomics and metabolic analysis, facilitating further exploration of the functional roles of natural products from ThT22.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Plant Sciences
Joel Abbey, Sherin Jose, David Percival, Laura Jaakola, Samuel K. Asiedu
Summary: Botrytis blight is a significant disease of wild blueberries, with symptoms varying among different phenotypes. The study found that the response of blueberry plants to B. cinerea varied in terms of gene expression and phenolic compound accumulation.
Article
Genetics & Heredity
Dandan Li, Xiaomei Li, Xintong Liu, Zhao Zhang
Summary: This study conducted a comprehensive genome-wide analysis of the bZIP gene family (RcbZIPs) in rose, revealing their phylogenetic relationships, gene structure, chromosome localizations, gene duplication, and potential functions. The expression analysis showed significant differential expression of 16 RcbZIPs in response to Botrytis cinerea infection, suggesting their candidate role in regulating rose's response to gray mold infection. Virus-induced gene silencing (VIGS) and overexpression (OE) studies demonstrated the involvement of RcbZIP17 in rose's resistance to gray mold. This study provides valuable information for further investigation into the function of the bZIP gene family in rose.
Article
Plant Sciences
Can Zhang, Muhammad Imran, Lu Xiao, Zhihong Hu, Guixiang Li, Fan Zhang, Xili Liu
Summary: The study revealed that Botrytis cinerea exhibited resistance to difenoconazole, with a slight increase in resistance levels between 2011 and 2016. Positive cross-resistance was only observed between difenoconazole and two DMIs, and isolates gradually shifted from single to multiple fungicide resistance over time.
Article
Biology
Marlene Henriquez-Urrutia, Rebecca Spanner, Consuelo Olivares-Yanez, Aldo Seguel-Avello, Rodrigo Perez-Lara, Hector Guillen-Alonso, Robert Winkler, Alfredo Herrera-Estrella, Paulo Canessa, Luis F. Larrondo
Summary: This study confirms the existence of circadian rhythms in Trichoderma atroviride and highlights the importance of these rhythms in the fungal-fungal interaction with Botrytis cinerea. The study also reveals the impact of clock components on various aspects of T. atroviride's lifestyle and the modulation of fungal interactions and dynamics.
Article
Multidisciplinary Sciences
G. Ramangouda, M. K. Naik, Rahul Nitnavare, Richa Yeshvekar, Joorie K. Bhattacharya, Pooja K. Bhatnagar-Mathur, Mamta K. Sharma
Summary: This study investigated the biocontrol activities and carbendazim tolerance of N-methyl-n-nitro-N-nitrosoguanidine (NTG) induced mutants of Trichoderma asperellum. Six NTG induced mutants were developed, with N2-3, N2-1, N1, and N2-2 showing the best antagonistic activities against fungi and volatile metabolite activities. Mutant N2-2 exhibited the highest carbendazim tolerance and was able to sporulate even at high concentrations of carbendazim. These mutants were found to have mutations in the tub2 gene, but the enhanced carbendazim tolerance did not affect their mycoparasitism and plant growth activity. Overall, the mutants showed similar attributes to the wild-type.
Article
Agriculture, Multidisciplinary
Gicele Sbardelotto De Bona, Simone Vincenzi, Fabiola De Marchi, Elisa Angelini, Nadia Bertazzon
Summary: The research found that chitosan (173/17) can effectively protect grapevines against Botrytis cinerea infection and induce plant defense response. This demonstrates the potential application value of chitosan (173/17) as a natural ecofriendly product for controlling Botrytis cinerea on grapevine.
JOURNAL OF PLANT DISEASES AND PROTECTION
(2021)
Article
Biotechnology & Applied Microbiology
B. E. Stummer, Q. Zhang, X. Zhang, R. A. Warren, P. R. Harvey
JOURNAL OF APPLIED MICROBIOLOGY
(2020)
Article
Biotechnology & Applied Microbiology
Qingxia Zhang, Belinda E. Stummer, Qinggang Guo, Wen Zhang, Xinjian Zhang, Liqun Zhang, Paul R. Harvey
Summary: Bacillus subtilis strain NCD-2 and Pseudomonas protegens strain FD6 produce antimicrobial metabolites effective against soil-borne plant pathogens. qPCR assays were used to monitor the populations of these inoculants in soil and rhizosphere, showing significant suppression of Rhizoctonia root rot in wheat seedlings. The study demonstrates the potential applications of these assays in controlling crop diseases.
BIOLOGICAL CONTROL
(2021)
Article
Entomology
Mei Liu, Xiaoyan Zhao, Xianxian Li, Xiaoqing Wu, Hongzi Zhou, Yunxiao Gao, Xinjian Zhang, Fangyuan Zhou
Summary: Recent studies have shown that insect-associated microbes play multiple roles such as lignin degradation, entomopathogen inhibition, and antibiotic production, which improve insect host fitness and offer potential for discovering beneficial microbes for industrial and agricultural applications. This study isolated and identified a total of 74 species of bacteria associated with D. antiqua, showing significant antifungal activity against phytopathogens including Fusarium moniliforme, Botryosphaeria dothidea, and Fusarium oxysporum strains. These bacteria, such as Ochrobactrum anthropi, Morganella morganii, Arthrobacter sp. 3, and Acinetobacter guillouiae, have the potential to be used as biocontrol agents against fungal phytopathogens.
JOURNAL OF ECONOMIC ENTOMOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Belinda E. Stummer, Xinjian Zhang, Hetong Yang, Paul R. Harvey
Summary: The study showed that strains A5MH and Tr906 could effectively suppress crown rot pathogen and promote wheat growth, while having distinct impacts on the structure of the rhizosphere soil fungal community.
BIOLOGICAL CONTROL
(2022)
Article
Environmental Sciences
Yunxiao Gao, Mei Liu, Xiaoyan Zhao, Xinjian Zhang, Fangyuan Zhou
Summary: This study revealed significant variation in the ability of soil microbes from different geographic locations to degrade neonicotinoid insecticide imidacloprid, and identified two promising bacterial candidates, Paracoccus and Achromobacter, through high throughput sequencing.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Editorial Material
Plant Sciences
Susu Fan, Fangyuan Zhou, Xueying Xie, Xinjian Zhang, Tielin Wang
Article
Microbiology
Fangyuan Zhou, Yunxiao Gao, Mei Liu, Letian Xu, Xiaoqing Wu, Xiaoyan Zhao, Xinjian Zhang
Summary: This study investigated the response of associated microbiota to a pathogen in Delia antiqua, showing that bacterial microbiota instead of fungal microbiota played a crucial role in inhibiting the pathogen during invasion, stabilizing the associated microbiota. These findings deepen our understanding of the mechanisms maintaining microbiota stability.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Mycology
Guang-Zhi Zhang, He-Tong Yang, Xin-Jian Zhang, Fang-Yuan Zhou, Xiao-Qing Wu, Xue-Ying Xie, Xiao-Yan Zhao, Hong-Zi Zhou
Summary: This study isolated five new species of Trichoderma from moist soils near a water source in different areas of China. These new species were identified based on morphological characteristics and phylogenetic analysis.
Review
Biotechnology & Applied Microbiology
Xiaoqing Wu, Yuping Lyu, He Ren, Fangyuan Zhou, Xinjian Zhang, Xiaoyan Zhao, Guangzhi Zhang, Hetong Yang
Summary: This study investigated the relationship between the elimination of oxalic acid (OA) by Trichoderma afroharzianum and its antagonistic effects on Botrytis cinerea. The results showed a correlation between OA degradation and mycoparasitism in T. afroharzianum, providing a new perspective on its antagonism against B. cinerea. The study also provided theoretical data for the scientific application of T. afroharzianum in biocontrol.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Article
Environmental Sciences
Mutian Wang, Xinjian Zhang, Yujie Huang, Fangyuan Zhou, Qingqing Zhao, Wen Zhang, Guanhong Chen, Yongchao Gao
Summary: A hydrocarbon-degrading bacterium Providencia rettgeri L1 was isolated from Shengli oil field, China, and its petroleum degradation characteristics were investigated. The study found that the strain showed a degradation rate of 45.9% in 14 days, and the degradation rate was accelerated when crude enzymes and formate dehydrogenase were combined. Analysis of the composition of the degraded petroleum showed significant degradation of saturates and aromatics, with different degradation rates for different chain lengths and ring numbers. The study also showed that the bacterium had a rapid proliferation advantage in culture media.
POLISH JOURNAL OF ENVIRONMENTAL STUDIES
(2022)
Article
Microbiology
Haiyang Yu, Xiaoqing Wu, Guangzhi Zhang, Fangyua Zhou, Paul R. Harvey, Leilei Wang, Susu Fan, Xueying Xie, Feng Li, Hongzi Zhou, Xiaoyan Zhao, Xinjian Zhang
Summary: Phosphorus-solubilizing bacteria (PSB) can convert insoluble phosphorus into soluble forms, improving the absorption and utilization of phosphorus by plants. This study identified a highly efficient PSB strain, JP233, and found that it could significantly reduce phosphorus leaching loss in soil while promoting maize growth.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Fangyuan Zhou, Xiaoqing Wu, Yunxiao Gao, Susu Fan, Hongzi Zhou, Xinjian Zhang
Summary: This study investigated the diversity shifts in the root microbiome of cucumber under different plant cultivation substrates and predicted the corresponding function of these different root bacterial microbiota. The results showed significant differences in alpha and beta diversity of samples from different substrates, as well as changes in abundance of specific bacterial genera and synthetic pathways in the root microbiome.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Genetics & Heredity
Linlin Wang, Fangyuan Zhou, Jianbo Zhou, Paul R. Harvey, Haiyang Yu, Guangzhi Zhang, Xinjian Zhang
Summary: This study sequenced and analyzed the genome of Pseudomonas sp. strain JP233, a bacterium known for its ability to solubilize phosphate and promote plant growth. Key genes associated with phosphate solubilization and plant-growth promotion were identified, providing a genetic basis for its potential applications in agriculture and industry.
Article
Environmental Sciences
Yali Zhang, Fanyong Song, Jianing Wang, Qingqing Zhao, Liwen Zheng, Zhenjie Wang, Xinjian Zhang, Yongchao Gao, Guanhong Chen, Yujie Huang
Summary: In this study, a strain of Acinetobacter vivianii KJ-1 capable of degrading diesel was obtained from oil-contaminated soil. After 12 days of incubation, strain KJ-1 was able to degrade over 40% of the diesel. The optimal diesel concentration and nitrogen source concentration for diesel degradation and bacterial growth were 4,140 mg/L and 350-700 mg/L, respectively. The optimal pH range for strain KJ-1 growth and diesel degradation was 6.5-8.0, while the optimal temperature range was 20-37℃. The analysis of the strain's complete genome revealed the potential mechanisms involved in diesel degradation. This study suggests that A. vivianii KJ-1 may be a useful candidate for bioremediation of diesel-polluted soil.
FRONTIERS IN ENVIRONMENTAL SCIENCE
(2022)
Article
Environmental Sciences
Fangyuan Zhou, Qingxia Liang, Xiaoyan Zhao, Xiaoqing Wu, Susu Fan, Xinjian Zhang
Summary: Pesticide resistance causes significant economic losses globally each year. Previous research has mainly focused on investigating the interactions between resistant pests and associated microbes, with a lack of quantification on the individual contributions of the pest host and associated microbes to resistance. This study compares resistant and sensitive strains and uses metaproteomics to analyze protein profiles, revealing contributions by the pest and its gut bacteria to phoxim resistance.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
