期刊
JOURNAL OF MEDICAL ENTOMOLOGY
卷 54, 期 4, 页码 1049-1054出版社
OXFORD UNIV PRESS INC
DOI: 10.1093/jme/tjx051
关键词
sand fly; bloodmeal identification; cytochrome b PCR
资金
- Fundacao de Amparo a Pesquisa do Estado de Minas Gerais/FAPEMIG [PPM-00438-14, BPD-00107-13]
- Conselho Nacional de Pesquisas/CNPq [301421/2013-7]
- Coordination of Training of Upper-Level Personnel (CAPES)
PCR-based identification of vertebrate host bloodmeals has been performed on several vectors species with success. In the present study, we used a previously published PCR protocol followed by DNA sequencing based on primers designed from multiple alignments of the mitochondrial cytochrome b gene used to identify avian and mammalian hosts of various hematophagous vectors. The amplification of a fragment encoding a 359bp sequence of the Cyt b gene yielded recognized amplification products in 192 female sand flies (53%), from a total of 362 females analyzed. In the study area of Casa Branca, Brazil, blood-engorged female sand flies such as Lutzomyia longipalpis (Lutz & Neiva, 1912), Migonemyia migonei (Franc, a, 1924), and Nyssomyia whitmani (Antunes & Coutinho, 1939) were analyzed for bloodmeal sources. The PCR-based method identified human, dog, chicken, and domestic rat blood sources.
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