Perfect 1JCH -resolved HSQC: Efficient measurement of one-bond proton-carbon coupling constants along the indirect dimension

A versatile (1)J(CH)resolved HSQC pulse scheme for the speedy, accurate and automated determination of one-bond proton-carbon coupling constants is reported. The implementation of a perfectBlRD element allows a straightforward measurement from the clean doublets obtained along the highly resolved Fl dimension, even for each individual (1)J(CHa) and (1)J(CHb) in diastereotopic HaCHb methylene groups. Real-time homodecoupling during acquisition and other alternatives to minimize accidental signal overlapping in overcrowded spectra are also discussed. (C) 2017 Elsevier Inc. All rights reserved.