Pure shift edited ultra high resolution NMR spectrum with complete eradication of axial peaks and unwanted couplings

Poor chemical shift dispersion and pairwise interaction among the entire coupled network of spins results in complex one dimensional H-1 NMR spectra, severely hampering the analysis and the accurate determination of (n)J(HH). Available two dimensional selective refocusing based techniques suffer from the evolution of undesirable couplings and intense axial peaks, creating ambiguity in the analysis and the extraction of coupling values. In this work, we report a novel two dimensional experiment for the complete elimination of axial peaks and unwanted couplings, while retaining only the couplings of the selected proton to its partners, with a blend of ultra-high resolution achieved by real time broad band homonuclear decoupling. (C) 2017 Elsevier Inc. All rights reserved.