Review
Biochemistry & Molecular Biology
Hsi-Hsien Lin
Summary: The specific extracellular interaction between GPI-anchored proteins and aGPCRs is crucial for unique biological functions. Despite their involvement in various biological processes, many members of these two protein families remain uncharacterized. The complex interaction and functional synergy between GPI-anchored proteins and aGPCRs have been extensively studied.
Review
Cell Biology
Ke Zhou
Summary: A polysaccharides-based cell wall plays a crucial role in shaping and protecting plant cells. Glycosylphosphatidylinositol (GPI)-anchored proteins (GPI-APs) are involved in the regulation of cell wall, acting as structural components and being responsible for organizing cellulose microfibrils. They also participate in cell wall integrity signaling transduction.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Guenter A. Mueller, Timo D. Mueller
Summary: This study investigated the intercellular transfer of GPI-APs, which found that full-length GPI-APs can be transferred between cells and regulate metabolism by upregulating lipid and glycogen synthesis. The transfer and synthesis were inhibited by serum proteins and promoted by PIGs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Guenter A. Mueller, Timo D. Mueller
Summary: Glycosylphosphatidylinositol (GPI)-anchored proteins (APs) are expressed in all eukaryotic organisms and exert main physiological roles in intercellular transfer via extracellular vesicles and lipoprotein-like particles. Non-vesicular transfer is controlled by various factors and can occur directly or indirectly. This transfer induces phenotypes such as lipid and glycogen synthesis, suggesting its non-genetic inheritance nature based on the transmission of matter rather than information.
Article
Biochemistry & Molecular Biology
Maria N. Balatskaya, Alexandra Baglay, Alexander Balatskiy
Summary: This study provided evidence supporting the presence of T-cadherin on platelets. The glycosylphosphatidylinositol anchor in the platelet T-cadherin form was confirmed. Furthermore, the study characterized T-cadherin with post-translational modifications as a new GPI-anchored protein on human platelets.
Article
Biochemistry & Molecular Biology
Mingyi Li, Jing Zheng, Xi He, Xinjun Zhang
Summary: In this study, we characterized Tiki proteins as GPI-anchored proteases and found that the GPI anchor determines their cellular localization and regulation by GDEs, but not their inhibitory activity on Wnt signaling.
Article
Food Science & Technology
Tomas B. Voisin, Emma C. Couves, Edward W. Tate, Doryen Bubeck
Summary: CD59 is a cell surface receptor that controls pore formation and inhibits the complement membrane attack complex (MAC). Despite blocking the MAC pores, CD59 is exploited by bacterial pore-forming proteins. Recent studies have shown differences in the orientation of CD59 in complexes with binding partners. This article demonstrates how GPI-anchored CD59 can accommodate diverse binding modes and identifies residues that interact with lipids.
Article
Infectious Diseases
Lena M. M. Sauer, Rodrigo Canovas, Daniel Roche, Hosam Shams-Eldin, Patrice Ravel, Jacques Colinge, Ralph T. T. Schwarz, Choukri Ben Mamoun, Eric Rivals, Emmanuel Cornillot
Summary: Protozoan parasites attach specific and diverse proteins to their plasma membrane via a GPI anchor. The FT-GPI software can detect GPI-anchored proteins and identify new candidates for vaccines against malaria and other parasitic diseases.
Review
Biochemistry & Molecular Biology
Guenter A. Mueller, Timo D. Mueller
Summary: Glycosylphosphatidylinositol-anchored proteins (GPI-APs) are anchored to the outer leaflet of plasma membranes through covalent linkage to a highly conserved glycolipid. They can be released into the surrounding environment, undergoing distinct arrangements upon loss of their GPI anchor or incorporation into various complexes. In mammals, the (patho)physiological roles of released GPI-APs depend on their release mechanisms, cell types, and tissues involved, and are controlled by their removal from circulation.
Article
Biotechnology & Applied Microbiology
Takamasa Fukunaga, Yuki Sakurai, Takao Ohashi, Yujiro Higuchi, Hiromi Maekawa, Kaoru Takegawa
Summary: Through a genome-wide screen of a deletion mutant strain in fission yeast, two genes (pwp1(+), SPBC1E8.05) encoding GPI-anchored proteins were identified to improve the growth rate of cells lacking the outer chain, thereby enhancing productivity of heterologous proteins. Mutational analysis revealed the importance of the GPI-anchored region of Pwp1p in attenuating the growth defect and supporting beta-glucan on the cell-surface of S. pombe non-enzymatically.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
Qin Liu, Xueyan Zhan, Dongfang Li, Junlong Zhao, Haiyong Wei, Heba Alzan, Lan He
Summary: A novel BgGPI52-WH antigen was identified as a potential immunodiagnostic marker and iELISA method was established for early diagnosis with good sensitivity. Clinical samples showed a positive rate of approximately 11.41%.
Article
Immunology
Eveline Snelders, Frederique Moyrand, Aude Sturny-Leclere, Frederique Vernel-Pauillac, Stevenn Volant, Guilhem Janbon, Alexandre Alanio
Summary: It is apparent that GPI-anchored proteins (GAPs) play a significant role in fungi, but the understanding of GAPs in Cryptococcus neoformans, a human opportunistic fungus, is still limited. By utilizing online GPI prediction tools, GAPs were identified and followed by the development of a mutant library for these GAP-encoding genes. A publicly available knock out (KO) mutant library was employed. A total of 41 overexpression and 34 KO mutants representing 47 unique genes were analyzed. Among the screened genes, two candidates, mannoprotein 88 (MP88) (CNAG_00776) and an uncharacterized protein (CNAG_00137), were further investigated by constructing additional independent mutant strains. The CNAG_00776 mutant displayed impaired growth under plasma membrane stress and significantly reduced phagocytosis. The CNAG_00137 mutant exhibited impaired growth during cell wall stress or increased temperature and a significant decrease in phagocytosis. Through a comprehensive genetic screen of GAPs in the genome of C. neoformans, two potential GAP genes involved in C. neoformans/host interaction and stress response were identified. Further exploration of these genes could lead to the discovery of new targets for antifungal drugs, treatment strategies, or vaccines to manage C. neoformans disease. (C) 2022 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.
MICROBES AND INFECTION
(2022)
Review
Biochemistry & Molecular Biology
Kunio Nakatsukasa
Summary: This article discusses the mechanisms of retro-translocation and degradation of misfolded proteins, as well as the quality control of glycosylphosphatidylinositol-anchored proteins. Recent studies suggest that ERAD may function as a fail-safe mechanism for the degradation of misfolded GPI-anchored proteins.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Microbiology
Marketa Samalova, Patricia Flamant, Remi Beau, Mike Bromley, Maryse Moya-Nilges, Thierry Fontaine, Jean-Paul Latge, Isabelle Mouyna
Summary: An analysis of the Aspergillus fumigatus genome has identified 86 genes coding for GPI-APs. These proteins have diverse functions in cell wall remodeling, virulence, and adhesion. The study focused on a new GPI-anchored protein called SwgA, which is found in the Clavati of Aspergillus and is involved in germination, growth, and morphogenesis, as well as nitrogen metabolism and thermosensitivity. The findings suggest that GPI-APs have broader roles in fungal metabolism beyond cell wall biosynthesis.
Review
Plant Sciences
Cai Yu Yu, Huan Kai Zhang, Ning Wang, Xin-Qi Gao
Summary: GPI-APs play important roles in the interactions between pollen/pollen tube and pistil tissues, affecting pollen germination on the stigma, pollen tube growth in the transmitting tract, pollen tube guidance to the ovule, and pollen tube reception in the embryo sac.
Article
Biochemistry & Molecular Biology
Aina Gotoh, Toshihiko Katoh, Yuta Sugiyama, Shin Kurihara, Yuji Honda, Haruko Sakurama, Taiho Kambe, Hisashi Ashida, Motomitsu Kitaoka, Kenji Yamamoto, Takane Katayama
CARBOHYDRATE RESEARCH
(2015)
Article
Biochemistry & Molecular Biology
Yuta Sugiyama, Toshihiko Katoh, Yuji Honda, Aina Gotoh, Hisashi Ashida, Shin Kurihara, Kenji Yamamoto, Takane Katayama
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2017)
Article
Biochemistry & Molecular Biology
Yuta Sugiyama, Aina Gotoh, Toshihiko Katoh, Yuji Honda, Erina Yoshida, Shin Kurihara, Hisashi Ashida, Hidehiko Kumagai, Kenji Yamamoto, Motomitsu Kitaoka, Takane Katayama
Article
Biochemistry & Molecular Biology
Mayo Sato, Dorothee Liebschner, Yusuke Yamada, Naohiro Matsugaki, Takatoshi Arakawa, Siobhan S. Wills, Mitchell Hattie, Keith A. Stubbs, Tasuku Ito, Toshiya Senda, Hisashi Ashida, Shinya Fushinobu
JOURNAL OF BIOLOGICAL CHEMISTRY
(2017)
Article
Biochemistry & Molecular Biology
Hisashi Ashida, Kana Tanigawa, Masashi Kiyohara, Toshihiko Katoh, Takane Katayama, Kenji Yamamoto
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2018)
Review
Biochemistry & Molecular Biology
Takayuki Higashiyama, Midori Umekawa, Masaya Nagao, Toshihiko Katoh, Hisashi Ashida, Kenji Yamamoto
CARBOHYDRATE RESEARCH
(2018)
Article
Biotechnology & Applied Microbiology
Yoshimi Shimada, Yuka Watanabe, Takura Wakinaka, Yoshihisa Funeno, Masayuki Kubota, Thida Chaiwangsri, Shin Kurihara, Kenji Yamamoto, Takane Katayama, Hisashi Ashida
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2015)
Article
Biotechnology & Applied Microbiology
Masahiro Komeno, Honoka Hayamizu, Kiyotaka Fujita, Hisashi Ashida
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2019)
Review
Microbiology
Toshihiko Katoh, Miriam N. Ojima, Mikiyasu Sakanaka, Hisashi Ashida, Aina Gotoh, Takane Katayama
Article
Biotechnology & Applied Microbiology
Yuki Sasaki, Masahiro Komeno, Akihiro Ishiwata, Ayako Horigome, Toshitaka Odamaki, Jin-Zhong Xiao, Katsunori Tanaka, Yukishige Ito, Kanefumi Kitahara, Hisashi Ashida, Kiyotaka Fujita
Summary: Gum arabic arabinogalactan protein (AGP) can be effectively degraded by the cooperative action of GAfase, exo-beta 1,3-galactanase (Bl1,3Gal), and BlArafE, leading to the production of alpha-L-Rhap-(1 -> 4)-beta-D-GlcpA-(1-6)-beta-D-Galp-(1 -> 6)-D-Gal tetrasaccharide. This oligosaccharide can be utilized by commensal bacteria, potentially facilitating cross-feeding interactions between bifidobacteria and other gut microbial groups.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Masahiro Komeno, Yuki Yoshihara, Junya Kawasaki, Wataru Nabeshima, Koshi Maeda, Yuki Sasaki, Kiyotaka Fujita, Hisashi Ashida
Summary: In this study, the degradation pathways of AX and AXOs by Bifidobacterium longum subsp. longum were characterized, and the crucial roles of BlArafD and BlArafE in the assimilation of AX were identified.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Endocrinology & Metabolism
Hideya Shintani, Hisashi Ashida, Tomoya Shintani
Summary: D-Glucosamine is considered beneficial for knee osteoarthritis and has potential for extending human lifespan, despite its controversial effects on knee joints. Recent studies have shown that glucosamine can affect joint function and possibly prolong lifespan by improving energy metabolism and inducing autophagy.
HUMAN NUTRITION & METABOLISM
(2021)
Article
Biochemistry & Molecular Biology
Hisashi Ashida, Taku Fujimoto, Shin Kurihara, Masayuki Nakamura, Masahiro Komeno, Yibo Huang, Takane Katayama, Takashi Kinoshita, Kaoru Takegawa
JOURNAL OF APPLIED GLYCOSCIENCE
(2020)
Article
Biochemistry & Molecular Biology
Toshihiko Katoh, Takako Maeshibu, Kei-ichi Kikkawa, Aina Gotoh, Yusuke Tomabechi, Motoharu Nakamura, Wei-Hsiang Liao, Masanori Yamaguchi, Hisashi Ashida, Kenji Yamamoto, Takane Katayama
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2017)