期刊
JOURNAL OF INVERTEBRATE PATHOLOGY
卷 150, 期 -, 页码 6-14出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jip.2017.08.015
关键词
Antheraea pernyi; Cathepsin O; Innate immune response; Expression pattern; Metamorphosis
类别
资金
- National Natural Science Foundation of China [31472147]
- Anhui Agricultural University [2017yjs-33]
- Natural Science Foundation of the Anhui Higher Education Institutions of China [KJ2017A145]
Cathepsins are key members of mammalian papain-like cysteine proteases that play an important role in the immune response. In this study, a fragment of cDNA encoding cathepsin O proteinase (ApCathepsin O) was cloned from Antheraea pernyi. It contains an open reading frame of 1170 bp and encodes a protein with 390 amino acid residues, including a conserved 129 inhibitor domain and a peptidase CIA (clan CA of cysteine proteases, papain family Cl subfamily) domain. Comparison with other previously reported cathepsin O proteins showed identity ranging from 45% to 79%. Quantitative real-time PCR (qRT-PCR) and Western blot analysis revealed that ApCathepsin O was highly expressed in the fat body; furthermore, the high expression during the pupal stage indicated that it might be involved during metamorphosis. After exposure to four different heat killed pathogens (Escherichia coli, Beauveria bassiana, Micrococcus luteus, and A. pernyi nucleopolyhedrovirus), the expression levels of ApCathepsin O mRNA significantly increased and showed variable expression patterns. This indicates that ApCathepsin O is potentially involved in the innate immune system of A. pernyi. Interestingly, ApCathepsin O expression was upregulated after 20-hydroxyecdysone (20E) injection, which suggested that it might be regulated by 20E. In conclusion, ApCathepsin O is a protease that may play an important role in the innate immune response and metamorphosis of A. pernyi.
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