Article
Biochemistry & Molecular Biology
Jin Song, Julia A. VanBuskirk, Shannath L. Merbs
Summary: This study reveals an important role of epigenetic regulation in controlling opsin expression, and demonstrates this through experiments using epigenetic modifiers in cell cultures and mouse retinal explants.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Cell Biology
Chao-Fan Lu, Ya-Nan Zhou, Jingjing Zhang, Songxue Su, Yupeng Liu, Guang-Hua Peng, Weidong Zang, Jing Cao
Summary: Photoreceptors play a crucial role in the retina by converting light into electrical signals. Epigenetics, specifically DNA methylation and histone modification, regulates the development, differentiation, degeneration, and pathological processes of photoreceptors. Understanding the role of methylation/demethylation in regulating photoreceptors in various physiological and pathological situations is essential for understanding retinal diseases and developing novel therapies.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Cell Biology
Yoshitaka Kubota, Hidekazu Nagano, Kentaro Kosaka, Hideyuki Ogata, Akitoshi Nakayama, Masataka Yokoyama, Kazutaka Murata, Shinsuke Akita, Motone Kuriyama, Nobutaka Furuyama, Masayuki Kuroda, Tomoaki Tanaka, Nobuyuki Mitsukawa
Summary: Ceiling culture-derived preadipocytes (ccdPAs) and adipose-derived stem cells (ASCs) were isolated from human subcutaneous fat tissue and showed differences in adipogenic potential due to variations in methylation and histone modification levels of adipogenesis-related genes, such as PPARc, fatty acid-binding protein 4, and leptin. Genomewide epigenetic analysis revealed distinct patterns in CpG methylation and H3K4me3 modification between ccdPAs and ASCs, suggesting epigenetic mechanisms underlying adipogenic differentiation capabilities of these cell types.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Xinran Yang, Chugang Mei, Sayed Haidar Abbas Raza, Xinhao Ma, Jianfang Wang, Jiawei Du, Linsen Zan
Summary: This study reveals a negative feedback regulatory loop between TET1 and METTL3 in myoblast differentiation, uncovering the interplay among DNA methylation, RNA methylation, and histone methylation in skeletal myogenesis.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Agriculture, Dairy & Animal Science
Pengfei Shi, Yong Ruan, Wenjiao Liu, Jinkui Sun, Jiali Xu, Houqiang Xu
Summary: The role of methylation in the promoter region of FOXO1 was investigated in regulating its transcriptional level, as well as its effect on the proliferation and differentiation of bovine myogenic cells. The study found that higher methylation levels in the FOXO1 promoter region led to lower mRNA expression of FOXO1. Silencing FOXO1 expression promoted the proliferation and differentiation of myoblasts.
Editorial Material
Medicine, Research & Experimental
James T. Pearson
Summary: Epigenome changes in chronic cardiovascular stress involve open chromatin and modifications of histone lysine residues. Set7 has been found to play an important role in cardiovascular remodeling and cardiac dysfunction.
Article
Cell Biology
Wei Dong, Wenjian Shi, Yongliang Liu, Jingwu Li, Yu Zhang, Guilan Dong, Xiaoliu Dong, Hua Gao
Summary: This study investigated the impact of CHST7 methylation status on the proliferation and differentiation of pituitary adenomas (PAs). Results showed that CHST7 levels were associated with the differentiation and cell adhesion of PAs, with low CHST7 levels linked to SF-1 lineage and optic chiasm compression. The study also revealed correlations between CHST7 and the eIF2/ATF4 pathway and mitochondrion-related genes.
Article
Cell Biology
Casandra Carrillo, Vagisha Ravi, Sarika Tiwari, Ellen A. Chernoff, Teri L. Belecky-Adams
Summary: The factors necessary for cell differentiation in the retina are not fully understood. This study focuses on the potential role of transforming growth factor beta-activated kinase 1 (TAK1) in the differentiation of cell types in the chick retina. The results indicate that TAK1 plays a unique role in differentiating and proliferating retinal cells.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Futaba Inoue, Kenbun Sone, Yusuke Toyohara, Yu Takahashi, Asako Kukita, Aki Hara, Ayumi Taguchi, Michihiro Tanikawa, Tetsushi Tsuruga, Yutaka Osuga
Summary: Epigenetic changes play a crucial role in endometrial cancer, including DNA methylation and histone modification. There are currently inhibitors targeting epigenetic regulators in preclinical studies and clinical trials.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Oncology
Jiaxin Liu, Jia-nan Li, Hongyu Wu, Panpan Liu
Summary: Epigenetic regulation plays a crucial role in lymphoma development and is closely associated with various malignancies. Understanding these epigenetic mechanisms can guide the development of clinical treatments.
FRONTIERS IN ONCOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Ruben Boers, Joachim Boers, Beatrice Tan, Marieke E. van Leeuwen, Evelyne Wassenaar, Erlantz Gonzalez Sanchez, Esther Sleddens, Yasha Tenhagen, Eskeatnaf Mulugeta, Joop Laven, Menno Creyghton, Willy Baarends, Wilfred F. J. van IJcken, Joost Gribnau
Summary: In this study, a bacterial methylation mark was used to label transcribed genes and active enhancers, allowing for tracking of cell state changes. This technique revealed rapid and coordinated activation of enhancers and nearby genes during enterocyte differentiation in the intestine. Furthermore, it provided insights into the chromatin landscape of intestinal stem cells and their differentiation process.
NATURE BIOTECHNOLOGY
(2023)
Article
Cell Biology
Jingjing Liu, Wen Lu, Dongyue Yan, Junyuan Guo, Li Zhou, Bimin Shi, Xiong Su
Summary: Mitochondrial functions are crucial for the metabolism and thermogenic status of brown adipocytes. The study explored the metabolic mechanisms linking mitochondrial oxidative phosphorylation (OXPHOS) to brown adipogenesis. OXPHOS deficiency inhibits brown adipogenesis by disrupting the transcription circuit without affecting ATP levels. Metabolomics analysis revealed decreased levels of tricarboxylic acid cycle intermediates and heme. Heme supplementation enhances complex I activity and partially reverses the inhibited brown adipogenesis by OXPHOS deficiency through histone demethylation.
Review
Genetics & Heredity
Reza Raeisossadati, Merari F. R. Ferrari, Alexandre Hiroaki Kihara, Issam AlDiri, Jeffrey M. Gross
Summary: This review highlights the importance of epigenetic regulation in the developing vertebrate retina, emphasizing its role in cell differentiation and disease development. It also suggests future studies should focus on investigating these mechanisms to develop novel treatment strategies for various retinal disorders.
EPIGENETICS & CHROMATIN
(2021)
Article
Multidisciplinary Sciences
Jianwei Lin, Yiping Wu, Gaofei Tian, Daqi Yu, Eunjeong Yang, Wai Hei Lam, Zheng Liu, Yihang Jing, Shangyu Dang, Xiucong Bao, Jason Wing Hon Wong, Yuanliang Zhai, Xiang David Li
Summary: H3K79me2 is an important epigenetic mark involved in gene regulation, cellular differentiation, and disease progression. A nucleosome-based photoaffinity probe was used to identify menin as a reader of H3K79me2 in a nucleosomal context. Cryo-electron microscopy showed that menin interacts with the nucleosome through specific domains and recognizes the methylation mark through a p-cation interaction. In cells, menin is selectively associated with H3K79me2 on chromatin, particularly in gene bodies.
Article
Biochemistry & Molecular Biology
Jung-Hoon Pyun, Byeong-Yun Ahn, Tuan Anh Vuong, Su Woo Kim, Yunju Jo, Jaehyung Jeon, Seung Ho Baek, Jaewon Kim, Sungsu Park, Gyu-Un Bae, Jun-Hyuk Choi, Jae-Ryong Kim, Dongryeol Ryu, Sang-Jin Lee, Jong-Sun Kang
Summary: Prmt1 plays a crucial role in maintaining vascular smooth muscle cell function, and its ablation may lead to aortic diseases. Ablation of Prmt1 affects the expression of contractile genes, causing VSMCs to switch phenotypes and resulting in aortic dilation and dissection.
EXPERIMENTAL AND MOLECULAR MEDICINE
(2021)
Article
Cell Biology
Alexandre Fort, Daisuke Yamada, Kosuke Hashimoto, Haruhiko Koseki, Piero Carninci
Meeting Abstract
Hematology
Ayana Kon, Satoshi Yamazaki, Keisuke Kataoka, Tetsuichi Yoshizato, Yusuke Shiozawa, Masashi Sanada, Kenichi Yoshida, Yasunobu Nagata, Yuichi Shiraishi, Satoru Miyano, Torsten Haferlach, Manabu Nakayama, Haruhiko Koseki, Hiromitsu Nakauchi, Seishi Ogawa
EXPERIMENTAL HEMATOLOGY
(2015)
Article
Immunology
Takeshi Inoue, Masahiro Morita, Atsushi Hijikata, Yoko Fukuda-Yuzawa, Shungo Adachi, Kyoichi Isono, Tomokatsu Ikawa, Hiroshi Kawamoto, Haruhiko Koseki, Tohru Natsume, Taro Fukao, Osamu Ohara, Tadashi Yamamoto, Tomohiro Kurosaki
JOURNAL OF EXPERIMENTAL MEDICINE
(2015)
Article
Genetics & Heredity
Stefan Schoenfelder, Robert Sugar, Andrew Dimond, Biola-Maria Javierre, Harry Armstrong, Borbala Mifsud, Emilia Dimitrova, Louise Matheson, Filipe Tavares-Cadete, Mayra Furlan-Magaril, Anne Segonds-Pichon, Wiktor Jurkowski, Steven W. Wingett, Kristina Tabbada, Simon Andrews, Bram Herman, Emily LeProust, Cameron S. Osborne, Haruhiko Koseki, Peter Fraser, Nicholas M. Luscombe, Sarah Elderkin
Article
Biology
Mitsuhiro Endoh, Takaho A. Endo, Jun Shinga, Katsuhiko Hayashi, Anca Farcas, Kit-Wan Ma, Shinsuke Ito, Jafar Sharif, Tamie Endoh, Naoko Onaga, Manabu Nakayama, Tomoyuki Ishikura, Osamu Masui, Benedikt M. Kessler, Toshio Suda, Osamu Ohara, Akihiko Okuda, Robert Klose, Haruhiko Koseki
Article
Biology
Emilia Dimitrova, Takashi Kondo, Angelika Feldmann, Manabu Nakayama, Yoko Koseki, Rebecca Konietzny, Benedikt M. Kessler, Haruhiko Koseki, Robert J. Klose
Article
Biochemistry & Molecular Biology
Anne H. Turberfield, Takashi Kondo, Manabu Nakayama, Yoko Koseki, Hamish W. King, Haruhiko Koseki, Robert J. Klose
NUCLEIC ACIDS RESEARCH
(2019)
Article
Immunology
Masahiro Kiuchi, Atsushi Onodera, Kota Kokubo, Tomomi Ichikawa, Yuki Morimoto, Eiryo Kawakami, Naoya Takayama, Koji Eto, Haruhiko Koseki, Kiyoshi Hirahara, Toshinori Nakayama
Summary: Research has shown that Cxxc1 plays a crucial role in regulating the transcription of a specific set of genes in CD4(+) T cells through epigenetic control, affecting the differentiation process of cells and thereby influencing the establishment of the T cell immune system.
JOURNAL OF EXPERIMENTAL MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Ni Zeng, Christophe M. Capelle, Alexandre Baron, Takumi Kobayashi, Severine Cire, Vera Tslaf, Cathy Leonard, Djalil Coowar, Haruhiko Koseki, Astrid M. Westendorf, Jan Buer, Dirk Brenner, Rejko Krueger, Rudi Balling, Markus Ollert, Feng Q. Hefeng
Summary: Loss of DJ-1 gene reduces signs of immune aging, providing a potential target for intervention in immune aging and aging-related diseases.
Article
Multidisciplinary Sciences
Kentaro Mochizuki, Jafar Sharif, Kenjiro Shirane, Kousuke Uranishi, Aaron B. Bogutz, Sanne M. Janssen, Ayumu Suzuki, Akihiko Okuda, Haruhiko Koseki, Matthew C. Lorincz
Summary: Germline genes are repressed by a combination of DNA methylation and histone marks, with PRC1.6 playing a key role in the pre-implantation stage. In contrast, GGD genes are hypermethylated in EpiLCs and their silencing is dependent upon SETDB1, PRC1.6/RING1B, and DNA methylation.
NATURE COMMUNICATIONS
(2021)
Article
Biology
Nina Tanaskovic, Mattia Dalsass, Marco Filipuzzi, Giorgia Ceccotti, Alessandro Verrecchia, Paola Nicoli, Mirko Doni, Daniela Olivero, Diego Pasini, Haruhiko Koseki, Arianna Sabo, Andrea Bisso, Bruno Amati
Summary: Loss of Mga and PRC1.6 has no significant impact on Myc-induced lymphomagenesis in transgenic mice, but loss of Pcgf6 accelerates tumor formation. This suggests an unexpected tumor suppressor activity of Pcgf6 independent of Mga and PRC1.6.
LIFE SCIENCE ALLIANCE
(2022)
Article
Multidisciplinary Sciences
Mengwen Hu, Yu-Han Yeh, Yasuhisa Munakata, Hironori Abe, Akihiko Sakashita, So Maezawa, Miguel Vidal, Haruhiko Koseki, Neil Hunter, Richard M. Schultz, Satoshi H. Namekawa
Summary: The Polycomb Repressive Complex 1 (PRC1) is crucial for the formation and maintenance of the ovarian reserve, which is essential for the female reproductive lifespan. Through establishing repressive chromatin states, PRC1 suppresses the gene expression program of meiotic prophase-I, enabling the transition to dictyate arrest and preventing the depletion of the ovarian reserve.
NATURE COMMUNICATIONS
(2022)
Article
Biology
Shuhei Uemura, Shoji Maenohara, Kimiko Inoue, Narumi Ogonuki, Shogo Matoba, Atsuo Ogura, Mayuko Kurumizaka, Kazuo Yamagata, Jafar Sharif, Haruhiko Koseki, Koji Ueda, Motoko Unoki, Hiroyuki Sasaki
Summary: In this study, the researchers found that the specific knockout of the Uhrf1 gene in oocytes affects chromosome segregation, cleavage division, and preimplantation viability in mice. Further investigations showed that these phenotypes were associated with cytoplasmic defects and not nuclear defects. Proteomic analysis of the knockout oocytes revealed down-regulation of proteins related to microtubules and disorganization of the cytoplasmic architecture, including mislocalization of mitochondria, endoplasmic reticulum, and maternal complex components.
LIFE SCIENCE ALLIANCE
(2023)
Meeting Abstract
Hematology
A. Kon, S. Yamazaki, K. Kataoka, T. Yoshizato, Y. Shiozawa, M. Sanada, K. Yoshida, Y. Yamazaki, Y. Shiraishi, S. Miyano, M. Nakayama, H. Koseki, H. Nakauchi, S. Ogawa