期刊
DEVELOPMENTAL NEUROBIOLOGY
卷 76, 期 6, 页码 673-687出版社
WILEY-BLACKWELL
DOI: 10.1002/dneu.22352
关键词
Kolmer-Agduhr; pet1; serotonin; spinal cord; tryptophan hydroxylase
资金
- National Institutes of Health [R01 NS065054, F31 NS083110, R25 GM096955, R25 NS083059]
- University of Minnesota Bob Allison Ataxia Research Center (BAARC)
- Program Chancengleichheit fur Frauen in Forschung und Lehre from the Bayerische Staatsregierung
- Julius-Maximilians-Universitat Wurzburg
Zebrafish intraspinal serotonergic neuron (ISN) morphology and distribution have been examined in detail at different ages; however, some aspects of the development of these cells remain unclear. Although antibodies to serotonin (5-HT) have detected ISNs in the ventral spinal cord of embryos, larvae, and adults, the only tryptophan hydroxylase (tph) transcript that has been described in the spinal cord is tph1a. Paradoxically, spinal tph1a is only expressed transiently in embryos, which brings the source of 5-HT in the ISNs of larvae and adults into question. Because the pet1 and tph2 promoters drive transgene expression in the spinal cord, we hypothesized that tph2 is expressed in spinal cords of zebrafish larvae. We confirmed this hypothesis through in situ hybridization. Next, we used 5-HT antibody labeling and transgenic markers of tph2-expressing neurons to identify a transient population of ISNs in embryos that was distinct from ISNs that appeared later in development. The existence of separate ISN populations may not have been recognized previously due to their shared location in the ventral spinal cord. Finally, we used transgenic markers and immunohistochemical labeling to identify the transient ISN population as GABAergic Kolmer-Agduhr double-prime (KA) neurons. Altogether, this study revealed a novel developmental paradigm in which KA neurons are transiently serotonergic before the appearance of a stable population of tph2-expressing ISNs. (c) 2015 Wiley Periodicals, Inc. Develop Neurobiol 76: 673-687, 2016
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