期刊
JOURNAL OF IMMUNOTHERAPY
卷 40, 期 3, 页码 83-93出版社
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1097/CJI.0000000000000151
关键词
exosomes; costimulatory; molecules; HLA; CTL; cross-dressing
资金
- Basic Science Research Program through the National Research Foundation of Korea (NRF) - Ministry of Education [2013R1A1A2065937]
- National Research Foundation of Korea [2013R1A1A2065937] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Dendritic cell-derived exosomes (DEX) comprise an efficient stimulator of T cells. However, the production of sufficient DEX remains a barrier to their broad applicability in immunotherapeutic approaches. In previous studies, genetically engineered K562 have been used to generate artificial antigen presenting cells (AAPC). Here, we isolated exosomes from K562 cells (referred to as CoEX-A2s) engineered to express human leukocyte antigen (HLA)-A2 and costimulatory molecules such as CD80, CD83, and 41BBL. CoEX-A2s were capable of stimulating antigen-specific CD8(+) T cells both directly and indirectly via CoEX-A2 crossdressed cells. Notably, CoEX-A2s also generated similar levels of HCMV pp65-specific and MART1-specific CD8(+) T cells as DEX in vitro. The results suggest that these novel exosomes may provide a crucial reagent for generating antigen-specific CD8(+) T cells for adoptive cell therapies against viral infection and tumors.
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