Article
Chemistry, Multidisciplinary
Edurne Pena-Mendizabal, Bruce K. Hua, Ethel Ibanez-Echevarria, Dolores Hernandez-Fernandez de Rojas, Angel Maquieira, Stuart L. Schreiber, Sergi Morais
Summary: In this study, we synthesized beta-lactam-derived haptens using the principles of diversity-oriented synthesis and discovered compounds for drug allergy in vitro testing. The haptens exhibited excellent diagnostic sensitivity and specificity in detecting specific anti-beta-lactam immunoglobulins. This strategy could potentially assist in beta-lactam delabeling initiatives.
CHEMICAL COMMUNICATIONS
(2022)
Review
Allergy
Shawn J. R. Goh, Johanna E. E. Tuomisto, Anthony W. Purcell, Nicole A. Mifsud, Patricia T. Illing
Summary: Penicillin, as a first-line treatment for infections, can induce immune-mediated adverse reactions including IgE-mediated and non-IgE-mediated reactions, manifesting as skin eruptions and liver injury. Studies have shown that the infiltration of T cells at the site of inflammation is dependent on the nature of the reaction.
Article
Infectious Diseases
Lara Munoz-Munoz, Jose A. Ainsa, Santiago Ramon-Garcia
Summary: This study evaluated the in vitro activity of various beta-lactam antibiotics against Mycobacterium kansasii (Mkn), a tuberculosis-like lung infection. Penicillins plus clavulanate and first- and third-generation cephalosporins were found to be the most active beta-lactams against Mkn. Combinatorial time-kill assays also showed favorable interactions of amoxicillin-clavulanate and cefadroxil with the standard Mkn treatment, making them potential alternative options for Mkn therapy.
Article
Oncology
Arthur J. Van de Vyver, Tina Weinzierl, Miro J. Eigenmann, Nicolas Frances, Sylvia Herter, Regula B. Buser, Jitka Somandin, Sarah Diggelmann, Florian Limani, Thorsten Lehr, Marina Bacac, Antje-Christine Walz
Summary: Targeted T-cell redirection, using T-cell bispecific antibodies (TCB), is a promising approach in cancer immunotherapy that activates T cells and kills tumor cells. A systems model was developed to understand how different levels of targeted antigen expression impact TCB activity, successfully predicting the cytotoxicity of TCBs across a wide range of tumor cell lines.
MOLECULAR CANCER THERAPEUTICS
(2021)
Article
Pharmacology & Pharmacy
Abdelbaset A. Elzagallaai, Awatif M. Abuzgaia, Blanca R. Del Pozzo-Magana, Eman Loubani, Michael J. Rieder
Summary: This study evaluated the role of the lymphocyte toxicity assay (LTA) in diagnosing and capturing serum sickness-like reactions (SSLR) due to beta-lactam antibiotics. The results showed a significant increase in cell death in patients' cells compared to healthy controls when incubated with the drug, indicating that the LTA test has strong potential as a useful diagnostic tool for beta-lactam-induced SSLR.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Immunology
Jimena Alvarez Freile, Yuzhu Qi, Lisa Jacob, Maria Franceskin Lobo, Harm Jan Lourens, Gerwin Huls, Edwin Bremer
Summary: Investigations into the strength of antigen-specific responses in vitro are crucial for early-phase research of immunotherapeutic approaches. This study presents a rapid luminescence-based method using HPV16 E7(11-20) peptide as a model antigen to evaluate MHC-dependent antigen-specific T cell responses in vitro. The method has important implications for assessing the impact of tumor microenvironment and immune checkpoint inhibitors, as well as accelerating the development of novel immunotherapies.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biology
Sayumi Fujimori, Izumi Ohigashi, Hayato Abe, Yosuke Matsushita, Toyomasa Katagiri, Makoto M. Taketo, Yousuke Takahama, Shinji Takada
Summary: The thymic epithelium plays a crucial role in supporting the development of T cells. This study found that enhanced expression of beta-catenin in thymic epithelial cells leads to thymic dysplasia and T cell deficiency in the embryonic period, while loss of beta-catenin function only slightly reduces cortical thymic epithelial cells and thymocytes in postnatal development.
Article
Infectious Diseases
Mingjia Peng, Renru Han, Yan Guo, Yonggui Zheng, Feifei Yang, Xiaogang Xu, Fupin Hu
Summary: This study investigated the in vitro combined inhibitory activities of three types of P-lactam antibiotics and clavulanic acid against bla(K)(PC)(2)-positive Klebsiella pneumoniae strains in China, finding that the highest combined inhibitory activity occurred at low concentration ratios. This suggests a potential new way to optimize the effects of antimicrobial regimens for infections caused by bla(K)(PC)(2)-positive K. pneumoniae strains.
INFECTION AND DRUG RESISTANCE
(2021)
Article
Biochemistry & Molecular Biology
Anna V. Izosimova, Diana V. Yuzhakova, Valeria D. Skatova, Lilia N. Volchkova, Elena V. Zagainova, Dmitry M. Chudakov, George V. Sharonov
Summary: The recent advances in cancer immunotherapy show great potential in solid tumor treatment, but distinguishing specific responses from nonspecific ones remains a key challenge. By identifying tumor-specific T lymphocytes and dissecting their repertoires, researchers have made progress in enhancing antitumor immunity and bypassing errors in dendritic cell generation. Utilizing an optimized protocol, a robust IFN gamma response was achieved in CD4+ T cells, with certain clones showing potential tumor specificity among IFN gamma-positive cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Immunology
Julieta Abraham-Miranda, Meghan Menges, Reginald Atkins, Mike Mattie, Justyna Kanska, Joel Turner, Melanie J. J. Hidalgo-Vargas, Frederick L. L. Locke
Summary: Cryopreservation of PBMCs allows for greater manufacturing flexibility without compromising CAR-T cell phenotype, activation, and anti-tumor cytotoxicity. This study supports the concept of using frozen PBMCs as a solution for the timing flexibility in CAR-T cell manufacturing.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Microbiology
Yu-Shan Huang, Pao-Yu Chen, Pei-Chun Chou, Jann-Tay Wang
Summary: Cefiderocol and aztreonam-avibactam (ATM-AVI) both showed activity against carbapenem-resistant Gram-negative bacilli, including those that produce metallo-beta-lactamases (MBLs). However, Enterobacteriaceae isolates producing NDM carbapenemase displayed lower susceptibility to both antibiotics.
MICROBIOLOGY SPECTRUM
(2023)
Article
Infectious Diseases
Moonsuk Bae, Taeeun Kim, Joung Ha Park, Seongman Bae, Heungsup Sung, Mi-Na Kim, Jiwon Jung, Min Jae Kim, Sung-Han Kim, Sang-Oh Lee, Sang-Ho Choi, Yang Soo Kim, Yong Pil Chong
Summary: Beta-lactam-avibactam combinations such as ceftazidime-avibactam and aztreonam-avibactam showed good in vitro activities against extended-spectrum beta-lactam-resistant Enterobacterales blood isolates, with lower inoculum effect frequencies compared to meropenem. Aztreonam-avibactam demonstrated better in vitro activity but was more susceptible to the inoculum effect than ceftazidime-avibactam, suggesting potential use as carbapenem-sparing strategies for treating infections.
Article
Infectious Diseases
Pimsumon Jiamboonsri, Chatchakorn Eurtivong, Sompit Wanwong
Summary: This study found that methyl gallate (MG) can synergize with beta-lactam antibiotics to combat methicillin-resistant Staphylococcus aureus (MRSA) infections. Additionally, gallic acid (GA) and MG were found to induce morphological changes in MRSA.
Article
Immunology
Avery J. Lam, Manjurul Haque, Kirsten A. Ward-Hartstonge, Prakruti Uday, Christine M. Wardell, Jana K. Gillies, Madeleine Speck, Majid Mojibian, Ramon I. Klein Geltink, Megan K. Levings
Summary: The study demonstrates that ablation of PTEN does not affect the overall function and stability of human Tregs, but selectively impairs their ability to suppress antigen-presenting cells. These findings highlight the functional necessity of PTEN-regulated PI3K-AKT activity for optimal human Treg function.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2022)
Article
Chemistry, Analytical
Marwa R. El-Zahry, Jenny Jeehan M. Nasr, Nora H. Al-Shaalan, Amer F. Mahmoud
Summary: The influence of metal complexation on two ss-lactam antibiotics was investigated using SERS technique. It was found that metal complexation can promote the degradation and change the antibacterial activity of the antibiotics.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Respiratory System
Janice Abbott, Margaret A. Hurley, Helen Chadwick, Daniel Peckham
Summary: This study found that optimistic coping has a positive impact on the survival of cystic fibrosis patients, while other coping strategies do not show significant effects. This finding is important for clinical interventions and improving quality of life and longevity.
JOURNAL OF CYSTIC FIBROSIS
(2023)
Article
Respiratory System
Rebecca J. Birch, Daniel Peckham, Henry M. Wood, Philip Quirke, Rob Konstant-Hambling, Keith Brownlee, Rebecca Cosgriff, Nicholas Burr, Amy Downing
Summary: It has been found that individuals with Cystic Fibrosis (CF) have a higher risk of developing colorectal cancer (CRC), and carriers of cystic fibrosis transmembrane conductance regulator (CFTR) mutations may also face an increased risk. With the increasing life expectancy of CF patients, more individuals are at risk of developing CRC.
JOURNAL OF CYSTIC FIBROSIS
(2023)
Review
Gastroenterology & Hepatology
L. R. Caley, H. White, M. C. de Goffau, R. A. Floto, J. Parkhill, B. Marsland, D. G. Peckham
Summary: Gut dysbiosis is evident in individuals with cystic fibrosis (CF), characterized by reduced diversity and taxonomic changes. The relationship between gut dysbiosis and systemic and lung inflammation is limited. The causes of gut dysbiosis are multifactorial and the impact of CFTR modulators on the gut microbiota is still unclear.
DIGESTIVE DISEASES AND SCIENCES
(2023)
Article
Respiratory System
L. R. Caley, C. Zagoya, F. Duckstein, H. White, D. Shimmin, A. M. Jones, J. Barrett, J. L. Whitehouse, R. A. Floto, J. G. Mainz, D. G. Peckham
Summary: This study compared gastrointestinal symptoms in patients with cystic fibrosis and cystic fibrosis-related diabetes. It found that individuals with cystic fibrosis-related diabetes have a higher burden of gastrointestinal symptoms, particularly bloating and nausea.
JOURNAL OF CYSTIC FIBROSIS
(2023)
Review
Critical Care Medicine
Daniel Peckham, Giulia Spoletini
Summary: The COVID-19 pandemic has accelerated the use of digital technologies in managing chronic diseases such as cystic fibrosis. Even with eased restrictions, these technologies like video and telephone consultations and remote monitoring are likely to remain crucial in the future of CF healthcare, thanks to improved clinical stability from effective modulator therapy.
SEMINARS IN RESPIRATORY AND CRITICAL CARE MEDICINE
(2023)
Article
Medicine, General & Internal
Felicity Liew, Shubha Talwar, Andy Cross, Brian J. Willett, Sam Scott, Nicola Logan, Matthew K. Siggins, Dawid Swieboda, Jasmin K. Sidhu, Claudia Efstathiou, Shona C. Moore, Chris Davis, Noura Mohamed, Jose Nunag, Clara King, A. A. Roger Thompson, Sarah L. Rowland-Jones, Annemarie B. Docherty, James D. Chalmers, Ling-Pei Ho, Alexander Horsley, Betty Raman, Krisnah Poinasamy, Michael Marks, Onn Min Kon, Luke Howard, Daniel G. Wootton, Susanna Dunachie, Jennifer K. Quint, Rachael A. Evans, Louise V. Wain, Sara Fontanella, Thushan I. de Silva, Antonia Ho, Ewen Harrison, J. Kenneth Baillie, Malcolm G. Semple, Christopher Brightling, Ryan S. Thwaites, Lance Turtle, Peter J. M. Openshaw
Summary: This study examined the nasal and plasma antibody responses in COVID-19 hospitalized patients one year after discharge and vaccination. The findings showed sustained elevated antibody responses in both nasal and plasma samples for at least 12 months, but the nasal antibody response was minimally influenced by vaccination. These findings highlight the importance of developing vaccines that enhance nasal immunity.
Review
Respiratory System
Caroline Reilly, Joe Sails, Antonios Stavropoulos-Kalinoglou, Rebecca J. Birch, Jim McKenna, Ian J. Clifton, Daniel Peckham, Karen M. Birch, Oliver J. Price
Summary: Physical inactivity is common in people with chronic airways disease and is associated with worse clinical outcomes and impaired quality of life. A systematic review and meta-analysis found that interventions promoting step-based physical activity, particularly those incorporating wearable activity monitors, resulted in a significant and clinically meaningful improvement in daily step count in pwCAD.
EUROPEAN RESPIRATORY REVIEW
(2023)
Review
Respiratory System
J. Stuart Elborn, Francesco Blasi, Pierre-Regis Burgel, Daniel Peckham
Summary: Recurrent and chronic bacterial infections are common in people with cystic fibrosis (CF). Antibiotics are the mainstay in treatment, but inhaled antibiotics have limited systemic adverse effects. In the past decade, highly effective CFTR modulators have become a new therapy that partially corrects chloride transport, improving lung function. While these modulators have a significant impact, they do not fully resolve chronic lung damage. This article reviews the role of inhaled antibiotics in the era of CFTR modulators.
EUROPEAN RESPIRATORY REVIEW
(2023)
Article
Allergy
Dylan Lawless, Hana Lango Allen, James E. D. Thaventhiran, Sarah Goddard, Oliver S. Burren, Evie Robson, N. I. H. R. BioResource Rare Dis Consortium NIHR BioResource Rare Dis Consortium, Daniel Peckham, Kenneth G. C. Smith, Sinisa Savic
Summary: This study analyzed the manifestation of cystic fibrosis (CF), a common genetic disorder, in patients with primary immunodeficiency (PID). The research found that patients carrying specific CFTR gene variants had worse outcome in terms of structural lung disease. Genome sequencing also identified cases of CFTR dysfunction in PID, suggesting the potential of precision medicine in treating traditional Mendelian disorders.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2023)
Article
Immunology
Anh Thu Dang, Christina Begka, Celine Pattaroni, Laura R. Caley, R. Andres Floto, Daniel G. Peckham, Benjamin J. Marsland
Summary: Short-chain fatty acids (SCFAs), produced from the microbial fermentation of dietary fiber, have an impact on cell metabolism and anti-inflammatory pathways. However, the administration of butyrate, a type of SCFA, affects neutrophil maturation and function in the bone marrow, resulting in a decreased ability to control bacterial infections in the lungs.
MUCOSAL IMMUNOLOGY
(2023)
Correction
Pharmacology & Pharmacy
Jochen G. Mainz, Carlos Zagoya, Louise Polte, Lutz Naehrlich, Lenny Sasse, Olaf Eickmeier, Christina Smaczny, Anton Barucha, Lilith Bechinger, Franziska Duckstein, Ludwik Kurzidim, Patience Eschenhagen, Laura Caley, Daniel Peckham, Carsten Schwarz
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Critical Care Medicine
Ahmet Z. Uluer, Gordon MacGregor, Pilar Azevedo, Veronica Indihar, Claire Keating, Marcus A. Mall, Edward F. McKone, Bonnie W. Ramsey, Steven M. Rowe, Ronald C. Rubenstein, Jennifer L. Taylor-Cousar, Elizabeth Tullis, Lael M. Yonker, Chenghao Chu, Anna P. Lam, Nitin Nair, Patrick R. Sosnay, Simon Tian, Fredrick Van Goor, Lakshmi Viswanathan, David Waltz, Linda T. Wang, Yingmei Xi, Joanne Billings, Alexander Horsley
Summary: This study demonstrates that once-daily dosing with vanzacaftor-tezacaftor-deutivacaftor is safe and effective in improving lung function and respiratory symptoms in patients with cystic fibrosis, and it enhances CFTR function.
LANCET RESPIRATORY MEDICINE
(2023)
Article
Critical Care Medicine
Callum Jackson, Iain Stewart, Tatiana Plekhanova, Peter S. Cunningham, Andrew L. Hazel, Bashar Al-Sheklly, Raminder Aul, Charlotte E. Bolton, Trudie Chalder, James D. Chalmers, Nazia Chaudhuri, Annemarie B. Docherty, Gavin Donaldson, Charlotte L. Edwardson, Omer Elneima, Neil J. Greening, Neil A. Hanley, Victoria C. Harris, Ewen M. Harrison, Ling-Pei Ho, Linzy Houchen-Wolloff, Luke S. Howard, Caroline J. Jolley, Mark G. Jones, Olivia C. Leavy, Keir E. Lewis, Nazir Lone, Michael Marks, Hamish J. C. McAuley, Melitta A. McNarry, Brijesh Patel, Karen Piper-Hanley, Krisnah Poinasamy, Betty Raman, Matthew Richardson, Pilar Rivera-Ortega, Sarah L. Rowland-Jones, Alex Rowlands, Ruth M. Saunders, Janet T. Scott, Marco Sereno, Ajay M. Shah, Aarti Shikotra, Amisha Singapuri, Stefan C. Stanel, Mathew Thorpe, Daniel G. Wootton, Thomas Yates, R. Gisli Jenkins, Sally J. Singh, William D-C Man, Christopher E. Brightling, Louise Wain, Joanna C. Porter, A. A. Roger Thompson, Alex Horsley, Philip L. Molyneaux, Rachael A. Evans, Samuel E. Jones, Martin K. Rutter, John F. Blaikley, PHOSP-COVID Study Collaborative Grp
Summary: This study investigated the prevalence and nature of sleep disturbance after discharge following hospital admission for COVID-19 and found that sleep disturbance is associated with dyspnoea, anxiety, and muscle weakness. Targeting sleep disturbance might be beneficial in treating the post-COVID-19 condition.
LANCET RESPIRATORY MEDICINE
(2023)
Article
Medicine, General & Internal
Hamish J. C. McAuley, Rachael A. Evans, Charlotte E. Bolton, Christopher E. Brightling, James D. Chalmers, Annemarie B. Docherty, Omer Elneima, Paul L. Greenhaff, Ayushman Gupta, Victoria C. Harris, Ewen M. Harrison, Ling-Pei Ho, Alex Horsley, Linzy Houchen-Wolloff, Caroline J. Jolley, Olivia C. Leavy, Nazir I. Lone, William D. C. Man, Michael Marks, Dhruv Parekh, Krisnah Poinasamy, Jennifer K. Quint, Betty Raman, Matthew Richardson, Ruth M. Saunders, Marco Sereno, Aarti Shikotra, Amisha Singapuri, Sally J. Singh, Michael Steiner, Ai Lyn Tan, Louise Wain, Carly Welch, Julie Whitney, Miles D. Witham, Janet Lord, Neil J. Greening, HOSP-COVID Study Collaborat Grp
Summary: This study recruited COVID-19 survivors in the UK and objectively measured frailty using FFP. The results showed that frailty and pre-frailty are common after hospitalization with COVID-19, and comprehensive assessment and interventions targeting frailty are needed beyond the initial illness.
Article
Health Care Sciences & Services
Bang Zheng, Giulia Vivaldi, Luke Daines, Olivia C. Leavy, Matthew Richardson, Omer Elneima, Hamish J. C. McAuley, Aarti Shikotra, Amisha Singapuri, Marco Sereno, Ruth M. Saunders, Victoria C. Harris, Linzy Houchen-Wolloff, Neil J. Greening, Paul E. Pfeffer, John R. Hurst, Jeremy S. Brown, Manu Shankar-Hari, Carlos Echevarria, Anthony De Soyza, Ewen M. Harrison, Annemarie B. Docherty, Nazir Lone, Jennifer K. Quint, James D. Chalmers, Ling-Pei Ho, Alex Horsley, Michael Marks, Krishna Poinasamy, Betty Raman, Liam G. Heaney, Louise V. Wain, Rachael A. Evans, Christopher E. Brightling, Adrian Martineau, Aziz Sheikh
Summary: This study investigated the determinants of recovery from dyspnoea in adults with COVID-19 and compared them to non-COVID-19 patients. The results showed that the factors associated with dyspnoea recovery in COVID-19 patients were similar to those in non-COVID-19 patients. These factors included age, gender, obesity status, mental health, cardiovascular disease, and length of hospital admission.
LANCET REGIONAL HEALTH-EUROPE
(2023)