Article
Respiratory System
Kathrin Kahnert, Franziska C. Trudzinski, Christiane Berger, Dieter Munker, Katrin Milger, Michael Irlbeck, Roland Tomasi, Christian Schneider, Sebastian Michel, Alessandro Ghiani, Felix J. F. Herth, Juergen Behr, Rudolf A. Joerres, Nikolaus Kneidinger
Summary: This study demonstrated that oxygenated hemoglobin (OxyHem) predicts the survival rate of lung transplant recipients after the onset of chronic lung allograft dysfunction (CLAD). OxyHem outperformed hemoglobin (Hb) or oxygen saturation in predicting survival. A reduction of 1 g/dL in OxyHem increased the risk of death after CLAD by 14%, and values below 11 g/dL corresponded to an 80% increase in mortality risk.
Review
Medicine, General & Internal
Anne-Laure Brun, Marie-Laure Chabi, Clement Picard, Francois Mellot, Philippe A. Grenier
Summary: Chronic lung allograft rejection, also known as CLAD, is a major cause of morbidity and mortality after lung transplantation. It consists of two main phenotypes, Bronchiolitis Obliterans Syndrome (BOS) and Restrictive Allograft Syndrome (RAS), each characterized by specific functional and imaging features. CT plays a crucial role in the diagnosis and follow-up of CLAD patients, helping to exclude identifiable causes, detect early abnormalities, differentiate between obstructive and restrictive phenotypes, and track disease progression.
Article
Nutrition & Dietetics
Sepideh Zununi Vahed, Elham Ahmadian, Peyman Foroughi, Soroush Mostafavi, Henning Madry, Mohammadreza Ardalan, Magali Cucchiarini
Summary: The study revealed a significant association between VDR and VDBP gene polymorphisms with renal allograft rejection and viral infection, showing differences in G allele frequencies among different groups.
Article
Surgery
Michael Y. Shino, Jamie L. Todd, Megan L. Neely, Jerry Kirchner, Courtney W. Frankel, Laurie D. Snyder, Elizabeth N. Pavlisko, Gregory A. Fishbein, Joanna M. Schaenman, Kristen Mason, Karen Kesler, Tereza Martinu, Lianne G. Singer, Wayne Tsuang, Marie Budev, Pali D. Shah, John M. Reynolds, Nikki Williams, Mark A. Robien, Scott M. Palmer, S. Sam Weigt, John A. Belperio
Summary: In this study, we investigated the relationship between plasma levels of CXCL9/CXCL10 and histopathologic lung allograft injuries, as well as the risk of developing CLAD. We found that elevated plasma CXCL9/CXCL10 levels were associated with injury patterns associated with CLAD, acute rejection, and acute lung injury, and there was a dose-response relationship between chemokine levels and CLAD risk. Importantly, the presence of elevated plasma chemokines was necessary for the association between histopathologic injury and CLAD. Similar associations and interactions were observed with CXCL9/CXCL10 levels in bronchoalveolar lavage fluid. Elevated plasma CXCL9/CXCL10 during allograft injury may contribute to CLAD development and could be a potential minimally invasive biomarker for immune monitoring.
AMERICAN JOURNAL OF TRANSPLANTATION
(2022)
Article
Critical Care Medicine
Jamie L. Todd, Jeremy M. Weber, Francine L. Kelly, Megan L. Neely, Hillary Mulder, Courtney W. Frankel, Andrew Nagler, Christopher Mccrae, Paul Newbold, Jim Kreindler, Scott M. Palmer
Summary: BALF eosinophilia is an independent predictor of future CLAD risk in lung transplant recipients, associated with acute rejection, lung injury, and pulmonary fungal detection. Established CLAD induces expression of type 2 inflammatory signals, highlighting the need for further research to clarify the role of type 2 pathway-specific interventions in CLAD prevention or treatment.
Article
Surgery
Eyal Fuchs, Liran Levy, Ella Huszti, Benjamin Renaud-Picard, Gregory Berra, Mitsuaki Kawashima, Akihiro Takahagi, Rasheed Ghany, Jan Havlin, Micheal C. McInnis, Shaf Keshavjee, Lianne G. Singer, Jussi Tikkanen, Chung-Wai Chow, Tereza Martinu
Summary: The study revealed that among patients with CLAD, approximately 22.2% experienced a phenotype transition, with 19 patients developing RLO phenotype. The development of RLO phenotype was associated with a shorter time to death/retransplant, especially for patients with Non-RLO phenotype at CLAD onset.
TRANSPLANT INTERNATIONAL
(2021)
Article
Medicine, General & Internal
Jesper Romhild Davidsen, Christian B. Laursen, Mikkel Hojlund, Thomas Kromann Lund, Klaus Nielsen Jeschke, Martin Iversen, Anna Kalhauge, Elisabeth Bendstrup, Jorn Carlsen, Michael Perch, Daniel Pilsgaard Henriksen, Hans Henrik Lawaetz Schultz
Summary: This study found that pleural thickening identified by lung ultrasound may serve as a potential surrogate marker to distinguish restrictive allograft syndrome (RAS) from bronchiolitis obliterans syndrome (BOS) in chronic lung allograft dysfunction (CLAD) patients after lung transplant. This discovery provides a new method for clinical diagnosis of CLAD patients post lung transplant.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Cardiac & Cardiovascular Systems
Nynke Wijbenga, Rogier A. S. Hoek, Bas J. Mathot, Leonard Seghers, Catharina C. Moor, Joachim G. J. V. Aerts, Daniel Bos, Olivier C. Manintveld, Merel E. Hellemons
Summary: Exhaled breath analysis using an electronic nose (eNose) is a promising novel biomarker for the diagnosis and phenotyping of chronic lung allograft dysfunction (CLAD). eNose technology could be a valuable addition to the diagnostic armamentarium for suspected graft failure in lung transplant recipients (LTR).
JOURNAL OF HEART AND LUNG TRANSPLANTATION
(2023)
Article
Surgery
Michael D. Parkes, Kieran Halloran, Alim Hirji, Shane Pon, Justin Weinkauf, Irina L. Timofte, Greg I. Snell, Glen P. Westall, Jan Havlin, Robert Lischke, Andrea Zajacova, Ramsey Hachem, Daniel Kreisel, Deborah Levine, Bartosz Kubisa, Maria Piotrowska, Stephen Juvet, Shaf Keshavjee, Peter Jaksch, Walter Klepetko, Philip F. Halloran
Summary: The study revealed that CLAD primarily reflects severe parenchymal injury-induced changes and dedifferentiation rather than inflammation. Fibrillar collagen genes were increased in CLAD, indicating matrix changes, and normal transcripts were decreased.
AMERICAN JOURNAL OF TRANSPLANTATION
(2022)
Article
Biochemistry & Molecular Biology
Francesca Lunardi, Daniela Isabel Abbrescia, Luca Vedovelli, Federica Pezzuto, Francesco Fortarezza, Giovanni Maria Comacchio, Vincenza Guzzardo, Pia Ferrigno, Monica Loy, Chiara Giraudo, Anna Sara Fraia, Eleonora Faccioli, Fausto Braccioni, Emanuele Cozzi, Dario Gregori, Geert M. Verleden, Fiorella Calabrese, Francesco Paolo Schena, Federico Rea
Summary: Chronic rejection is the leading cause of reduced survival and quality of life in lung transplant patients. This study identified a distinct molecular signature in lung biopsies of patients with histological signs of chronic rejection, with LCN2 gene playing a central role. The findings suggest that LCN2 gene may be crucial in the pathophysiology of chronic rejection.
Article
Multidisciplinary Sciences
Cecilia Veraar, Jonathan Kliman, Alberto Benazzo, Felicitas Oberndorfer, Maria Laggner, Philipp Hacker, Thomas Raunegger, Stefan Janik, Peter Jaksch, Walter Klepetko, Hendrik J. Ankersmit, Bernhard Moser
Summary: The study investigated biomarker differences between different phenotypes of Chronic Lung Allograft Dysfunction (CLAD), identifying the potential value of Lipocalin-2 and Activin-A in distinguishing between BOS, RAS, and ARAD patients, as well as their potential as predictors of CLAD prognosis.
SCIENTIFIC REPORTS
(2021)
Review
Immunology
Saskia Bos, Andrew J. Filby, Robin Vos, Andrew J. Fisher
Summary: This study comprehensively assesses the effector immune cells in lung tissue and bronchoalveolar lavage fluid from lung transplant recipients with Chronic Lung Allograft Dysfunction (CLAD). The research reveals the complexity of innate and adaptive immune cell responses in CLAD, along with the presence of immune cell products associated with tissue remodeling. Neutrophils and eosinophils are found to be linked to CLAD incidence, with eosinophils specifically predisposing to restrictive allograft syndrome. Cytotoxic and T-helper cells play a significant role in CLAD pathogenesis, although their specific role in tissue processes remains unclear from predominantly bronchoalveolar lavage data. Restrictive allograft syndrome shows a more pronounced humoral immune involvement. The evaluation of published studies over the last 20 years highlights the complex immunopathology of CLAD and emphasizes the need for more single-cell resolution spatial studies in lung tissue from CLAD patients.
Article
Respiratory System
Dong Tian, Xiangyun Zheng, Hongtao Tang, Heng Huang, Junjie Wang, Lin Xu, Caihan Li, Haoji Yan, Ruixuan Yu, Jinzhu Nan, Menggen Liu, Xiaoguang Guo, Shunhai Jian, Tao Wang, Senyi Deng, Qiang Pu, Lunxu Liu
Summary: Metformin, an oral hypoglycemic agent, attenuates chronic lung allograft dysfunction (CLAD) in rat models through its antifibrotic and immunomodulatory effects. The activation of AMPK may play a role in the mechanism. These findings provide experimental rationale for further clinical trials.
RESPIRATORY RESEARCH
(2023)
Article
Surgery
Naofumi Miyahara, Alberto Benazzo, Felicitas Oberndorfer, Akinori Iwasaki, Viktoria Laszlo, Balasz Dome, Mir Ali Hoda, Peter Jaksch, Walter Klepetko, Konrad Hoetzenecker
Summary: miR-21 expression was higher in restrictive allograft syndrome (RAS) than in bronchiolitis obliterans syndrome (BOS) specimens, with a significant increase in serum concentration over time in RAS patients. Vimentin, NICD, and p-SMAD 2/3 were positive in a higher proportion of BOS patients compared to RAS cases.
TRANSPLANT INTERNATIONAL
(2022)
Article
Respiratory System
Miriana D'Alessandro, Laura Bergantini, Antonella Fossi, Elda De Vita, Felice Perillo, Luca Luzzi, Piero Paladini, Piersante Sestini, Paola Rottoli, Elena Bargagli, David Bennett
Summary: This study found that galectin-1 concentrations were higher in lung transplant patients with bronchiolitis obliterans syndrome (BOS) and may serve as clinical biomarkers for diagnosis and prognosis. Galectin-3 showed a negative correlation with DLCO% and KCO% in stable lung transplant patients, while Gal-9 showed a positive correlation with KCO%.
Review
Medical Laboratory Technology
Sophie Steels, Marijke Proesmans, Xavier Bossuyt, Lieven Dupont, Glynis Frans
Summary: This paper reviews the diagnostic criteria for allergic bronchopulmonary aspergillosis (ABPA) as well as novel research biomarkers that can be used for the diagnosis and treatment follow-up of ABPA in cystic fibrosis.
CRITICAL REVIEWS IN CLINICAL LABORATORY SCIENCES
(2023)
Editorial Material
Cardiac & Cardiovascular Systems
Jan Van Slambrouck, Marianne S. Carlon, Dirk Van Raemdonck, Laurens J. Ceulemans
JOURNAL OF THORACIC AND CARDIOVASCULAR SURGERY
(2023)
Article
Surgery
Cedric Vanluyten, Christelle M. Vandervelde, Robin Vos, Jan Van Slambrouck, Steffen Fieuws, Paul De Leyn, Philippe Nafteux, Herbert Decaluwe, Hans Van Veer, Lieven Depypere, Yanina Jansen, An-Lies Provoost, Arne P. Neyrinck, Catherine Ingels, Bart M. Vanaudenaerde, Laurent Godinas, Lieven J. Dupont, Geert M. Verleden, Dirk Van Raemdonck, Laurens J. Ceulemans
Summary: This study retrospectively analyzed single-center bilateral lung transplantations performed between 2010 and 2020. The results showed that lung transplantation from donors aged ≥70 years is feasible and safe, with comparable short and long-term outcomes to transplantation from younger donors.
Article
Virology
Benedict Vanlerberghe, Casper Vrij, Kris Bogaerts, Pieter Vermeersch, Katrien Lagrou, Geert Molenberghs, Filip Rega, Laurens J. Ceulemans, Dirk van Raemdonck, Ina Jochmans, Diethard Monbaliu, Jacques Pirenne, Tim Vanuytsel, Pieter Gillard, Helene Schoemans, Johan Van Cleemput, Dirk Kuypers, Robin Vos, Frederik Nevens, Jef Verbeek
Summary: This observational study investigated the effectiveness of SARS-CoV-2 vaccines and breakthrough infections in transplant recipients. It found significant variations in antibody response rates and rates of severe infections among different transplant types, which were modulated by specific risk factors. These findings highlight the importance of tailored COVID-19 prevention measures for transplant recipients.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Article
Medicine, Research & Experimental
Saartje Uyttebroek, Lieven Dupont, Mark Jorissen, Laura Van Gerven
Summary: The study aimed to evaluate the burden of CF-related CRS in patients with CF. The results showed a high prevalence of CF-CRS in the cohort, with only a minority of patients having controlled disease. Patients receiving CFTR modulators had significantly lower CT scores and less bacterial colonization.
Article
Cardiac & Cardiovascular Systems
Bonnie T. Chao, Andrew T. Sage, Jonathan C. Yeung, Xiaohui Bai, Jin Ma, Tereza Martinu, Mingyao Liu, Marcelo Cypel, Dirk Van Raemdonck, Laurens J. Ceulemans, Arne Neyrinck, Stijn Verleden, Shaf Keshavjee
Summary: This study investigated lung inflammatory biomarkers using human lung tissue biopsies and ex vivo lung perfusion perfusate. The findings suggest that lung biopsies are consistent in gene expression and reflect the entire lung, except when collected from the lingula or an area of focal injury. Additionally, ex vivo lung perfusion perfusate provides a representative measurement of lung inflammation. These results are important for clinicians in selecting donor lungs.
JOURNAL OF THORACIC AND CARDIOVASCULAR SURGERY
(2023)
Article
Immunology
Charlotte de Fays, Vincent Geudens, Iwein Gyselinck, Pieterjan Kerckhof, Astrid Vermaut, Tinne Goos, Marie Vermant, Hanne Beeckmans, Janne Kaes, Jan Van Slambrouck, Yousry Mohamady, Lynn Willems, Lucia Aversa, Emanuela E. Cortesi, Charlotte Hooft, Gitte Aerts, Celine Aelbrecht, Stephanie Everaerts, John E. McDonough, Laurens J. De Sadeleer, Sophie Gohy, Jerome Ambroise, Wim Janssens, Laurens J. Ceulemans, Dirk Van Raemdonck, Robin Vos, Tillie L. Hackett, James C. Hogg, Naftali Kaminski, Ghislaine Gayan-Ramirez, Charles Pilette, Bart M. Vanaudenaerde
Summary: This study investigated the mucosal immune defense in control and end-stage COPD lungs, and found dysregulation in mucosal defense, with decreased defensins and increased mucus and IgA responses. The study also observed CD8+ T-cell accumulation in mild emphysema zones and adaptive Th and B cell activation in areas with more advanced tissue destruction. The study suggests that innate immune alterations in COPD occur early in the tissue destruction process, affecting both the alveoli and the terminal bronchioles, before the onset of an adaptive immune response.
FRONTIERS IN IMMUNOLOGY
(2023)
Meeting Abstract
Cardiac & Cardiovascular Systems
M. Orlitova, D. Van Beersel, A. E. Frick, K. Van de Voorde, K. Degezelle, J. Hellinck, M. Nolmans, B. M. Vanaudenaerde, G. M. Verleden, R. Vos, P. Claus, D. E. Van Raandonck, L. J. Ceulemans, T. Verbelen, A. P. Neyrinck
JOURNAL OF HEART AND LUNG TRANSPLANTATION
(2023)
Meeting Abstract
Cardiac & Cardiovascular Systems
H. Beeckmans, P. Kerckhof, J. McDonough, L. De Sadeleer, J. Kaes, A. Sacreas, C. Aelbrecht, A. Vanstapel, K. Maes, H. Schoemans, E. Wauters, A. Neyrinck, G. Verleden, L. Dupont, L. Godinas, D. Van Raemdonck, B. Vanaudenaerde, R. Vos
JOURNAL OF HEART AND LUNG TRANSPLANTATION
(2023)
Meeting Abstract
Cardiac & Cardiovascular Systems
N. D. de Manna, D. Van Raemdonck, M. Hartwig, B. Bottiger, G. Loor, A. Leon, M. Villavicencio, N. Langer, A. Emtiazjoo, S. Chandrashekaran, A. Neyrinck, Y. Toyoda, A. Kashem, S. Huddleston, P. Sanchez, K. Subramaniam, G. Warnecke, F. Ius
JOURNAL OF HEART AND LUNG TRANSPLANTATION
(2023)
Meeting Abstract
Cardiac & Cardiovascular Systems
C. Hooft, J. Kaes, T. Heigl, H. Beeckmans, P. Kerckhof, A. Vanstapel, X. Jin, J. Slambrouck, C. Vandervelde, D. Van Raemdonck, N. Kaminski, J. McDonough, L. Ceulemans, R. Vos, B. Vanaudenaerde
JOURNAL OF HEART AND LUNG TRANSPLANTATION
(2023)
Meeting Abstract
Cardiac & Cardiovascular Systems
J. Kaes, E. Vanhulle, L. Seldeslachts, C. Hooft, H. Beeckmans, X. Jin, P. Kerckhof, J. Van Slambrouck, D. Van Raemelonck, G. Vande Velde, L. Naesens, D. Schols, L. J. Ceulemans, R. Vos, K. Vertneire, B. M. Vanaudenaerde
JOURNAL OF HEART AND LUNG TRANSPLANTATION
(2023)
Article
Biochemical Research Methods
Jan Van Slambrouck, Charlotte Schoenaers, Lies Laenen, Xin Jin, Kurt Beuselinck, Ann Verdonck, Joost Wauters, Geert Molenberghs, Bart M. Vanaudenaerde, Robin Vos, Peter Mombaerts, Katrien Lagrou, Laurens J. Ceulemans
Summary: The ability to detect SARS-CoV-2 RNA or antigen in BAL fluid samples using point-of-care tests (POCTs) can reduce the risk of developing severe COVID-19 after lung transplantation.
JOURNAL OF VIROLOGICAL METHODS
(2024)
