期刊
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
卷 48, 期 1, 页码 95-101出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.dci.2014.09.008
关键词
Zebrafish RIG-I; NF-kappa B signaling pathway; IFN signaling pathway; TRIM25 regulation
资金
- Hi-Tech Research and Development Program of China (863) [2012AA092202]
- National Basic Research Program of China (973) [2012CB114404, 2012CB114402]
- National Natural Science Foundation of China [31172436, 31272691, 31372554, 31472298]
- Program for Key Innovative Research Team of Zhejiang Province [2010R50026]
- Scientific Research Fund of Zhejiang Provincial Science and Technology Department [2013C12907-9]
The retinoic acid-inducible gene I (RIG-I) is a critical sensor for host recognition of RNA virus infection and initiation of antiviral signaling pathways in mammals. However, data on the occurrence and functions of this molecule in lower vertebrates are limited. In this study, we characterized an RIG-I homolog (DrRIG-I) from zebrafish. Structurally, this DrRIG-I shares a number of conserved functional domains/motifs with its mammalian counterparts, namely, caspase activation and recruitment domain, DExD/H box, a helicase domain, and a C-terminal domain. Functionally, stimulation with DrRIG-I CARD in zebrafish embryos significantly activated the NF-kappa B and IFN signaling pathways, leading to the expression of TNF-alpha, IL-8 and IFN-induced Mx, ISG15, and viperin. However, knockdown of TRIM25 (a pivotal activator for RIG-I receptors) significantly suppressed the induced activation of IFN signaling. Results suggested the functional conservation of RIG-I receptors in the NF-kappa B and IFN signaling pathways between teleosts and mammals, providing a perspective into the evolutionary history of RIG-I-mediated antiviral innate immunity. (C) 2014 Elsevier Ltd. All rights reserved.
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