Article
Immunology
Dianjiang Yu, Yufeng Zhai, Peimin He, Rui Jia
Summary: This study applied transcriptomic and metabolomic analysis to investigate the effects of WSSV challenge on L. vannamei hepatopancreas. The results showed that WSSV disrupts the immune system and causes metabolic disorder in L. vannamei.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Environmental Sciences
Yufen Li, Ruixue Tong, Zeyuan Li, Xin Zhang, Luqing Pan, Yaobing Li, Ning Zhang
Summary: In this study, RNA interference was used to investigate the effects of ammonia exposure on haematopoiesis and apoptosis in Litopenaeus vannamei. The results showed that ammonia stress reduced the total haemocytes count and knock-down of astakine (AST) further decreased it. Additionally, ammonia stress induced oxidative stress, leading to DNA damage and up-regulated expression of death receptor, mitochondrial, and endoplasmic reticulum stress pathways. Overall, this study enhances our understanding of risk management in shrimp aquaculture.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Fisheries
Xing-Chao Ren, Xiao-Yuan Wan, Qing-Hui Liu
Summary: Ribosomes are crucial in the antiviral immune response of Litopenaeus vannamei, with LvRPL4-L and LvRPS16 showing increased expression after WSSV infection. Silencing of LvRPL4-L or LvRPS16 led to significantly higher viral copy numbers and mortality rates. This study provides insight into the role of LvRPL4-L and LvRPS16 in the immune response of L. vannamei against WSSV.
AQUACULTURE REPORTS
(2021)
Article
Fisheries
Shuang Zhang, Cuihong Hou, Bang Xiao, Yuanmao Yao, Wei Xiao, Chaozheng Li, Lili Shi
Summary: The LvArasin-like peptide is identified from the hemocytes of Pacific white shrimp and exhibits antimicrobial activity against both gram-positive and gram-negative bacteria. Its expression is up-regulated in response to bacterial infections, suggesting its involvement in host defense. LvArasin-like has the potential to be a therapeutic agent for disease control in shrimp aquaculture.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2021)
Article
Fisheries
Yi-Lun Chen, Ramya Kumar, Chun-Hung Liu, Han-Ching Wang
Summary: Peritrophins play essential roles in the porosity and strength of the peritrophic membrane in the shrimp midgut and have a crucial role in determining the colonization and tissue distribution of AHPND-causing bacteria. The binding of LvPeritrophin to WSSV and AHPND-causing bacteria was confirmed, and silencing of LvPeritrophin inhibited WSSV gene expression but increased bacterial colonization. The findings contribute to our understanding of host-pathogen interactions and can be applied in developing strategies for shrimp aquaculture.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Yongxiong Huang, Qi Li, Yunhao Yuan, Zhiqiang Zhang, Baijian Jiang, Shiping Yang, Jichang Jian
Summary: This study elucidated the role of Nrf2 in the regulation of antioxidant capacity, oxidative stress, apoptosis, and autophagy in shrimp. Evidence of gene expression and enzymatic activity was provided.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Sheng Wang, Haoyang Li, Peng Zhu, Qihui Fu, Bin Yin, Qinyao Li, Rongjian Chen, Xiewu Jiang, Shaoping Weng, Jianguo He, Chaozheng Li
Summary: LvMAPKKK15, a homolog of MAPKKK15 in shrimp, is abundantly expressed in early development stages and in eyestalk, nerve, and hemocytes. It responds to challenges of Poly (I: C) and WSSV, and its knockdown leads to decreased sensitivity to WSSV infection in shrimp. LvMAPKKK15 can up-regulate WSSV immediate early genes and suppress anti-viral factors LvVago1-5, indicating its role in WSSV pathogenesis.
Article
Fisheries
Yiwen Jian, Lingwei Ruan, Wenyang Lin, Jie Ran, Yalin Yang, Hong Shi
Summary: TEAD family transcription factors play a vital regulatory role in cell proliferation, apoptosis, tumor formation, and antiviral immunity. In this study, a novel homolog of the TEAD family, LvScalloped, was cloned and characterized from Litopenaeus vannamei. LvScalloped was found to be up-regulated in response to WSSV infection, and knockdown of LvScalloped reduced viral gene transcription and mortality in L. vannamei. Further investigation showed that LvScalloped can activate the immediate-early viral gene promoter and interact with LvYorkie to regulate cell viability. This study provides insights into the virus-host interactions and the functional mechanism of the Hippo signaling pathway in shrimp.
Article
Fisheries
Jie Ren, Wenyang Lin, Hong Shi, Yiwen Jian, Lingwei Ruan
Summary: The Hippo signaling pathway, including its core molecule Yorkie, plays important roles in organ size control, development, tumorigenesis, and immunity. In this study, a Yorkie homolog named LvYKI was cloned and characterized from Litopenaeus vannamei. LvYKI showed different regulatory mechanisms in different tissues and was involved in white spot syndrome virus (WSSV) infection. Knocking down of LvYKI inhibited the transcription of WSSV genes and delayed shrimp mortality, while increasing hemocyte apoptosis. Moreover, LvYKI was found to interact with Lv beta-catenin, linking the Wnt and Hippo signaling pathways in innate immunity. This study provides insights into the role of LvYKI in the interaction between shrimp and virus, enhancing our understanding of the molecular mechanism in innate immunity.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Qiang Fu, Kun Sun, Juan Sui, Xupeng Li, Jiawang Cao, Jian Tan, Baolong Chen, Kun Luo, Sheng Luan, Jie Kong, Xianhong Meng
Summary: This study investigated the performance differences among offspring obtained by intra- and inter-strain crosses using two shrimp strains, A and B, with outstanding WSSV resistance and growth, respectively. The results showed that crossbreeding can complement the advantages of different strains, with the cross of strain BA performing the best in terms of WSSV resistance and growth. The heritability estimates of body length and weight were medium, and there were significant genetic and phenotypic correlations among growth-related traits. The study provides important reference data for breeding disease-resistant and fast-growing shrimp strains.
AQUACULTURE REPORTS
(2023)
Article
Virology
Xu Zhang, Li-Peng Shan, Qi Zhao, Lei Liu, Xu OuYang, Yang Hu, Chen-Jie Fei, Jiong Chen
Summary: This study evaluated and screened active molecules from Chinese herbs against WSSV and found that taxifolin had the highest inhibition rate. Taxifolin treatment decreased the infectivity and genome copy number of WSSV, reduced shrimp mortality, and blocked horizontal transmission of the virus. Additionally, taxifolin enhanced the innate immunity of shrimp and improved their resistance to WSSV. These results suggest that taxifolin has the potential to block WSSV outbreak and reduce shrimp culture loss.
Article
Fisheries
Zhifei Chen, Depeng Fan, Luqing Pan, Chen Su, Yanjun Ding, Mingxiang Lu
Summary: This study found that dietary quercetin improved the growth performance of Litopenaeus vannamei shrimp, enhanced antioxidant capability and immune response, regulated the composition and structure of intestinal microbiota, and reduced cumulative mortality after white spot syndrome virus challenge.
Article
Zoology
Rui-Feng Zou, Xing-Chao Ren, Qing-Hui Liu
Summary: The study cloned the full-length cDNA sequence of LvSPARC-L from shrimp, and indicated that LvSPARC-L might play an antiviral role by regulating apoptosis.
JOURNAL OF INVERTEBRATE PATHOLOGY
(2021)
Article
Fisheries
Zi-ang Wang, Jiefu Zheng, Linwei Yang, Hongliang Zuo, Shengwen Niu, Shaoping Weng, Jianguo He, Xiaopeng Xu
Summary: Research on the Pacific white shrimp LvWnt11 gene show that it plays different roles in immune responses against Vibrio parahaemolyticus and white spot syndrome virus infections. LvWnt11 regulates expression of various immune-related factors and may help to uncover immune mechanisms in crustaceans.
Article
Endocrinology & Metabolism
Bong Jung Kang, Zakea Sultana, Marcy N. Wilder
Summary: Vitellogenesis-inhibiting hormone (VIH) negatively regulates reproduction in shrimp, and transcriptional silencing of Liv-SGP-G was successfully achieved with dsRNA injections. Although the treatment did not promote ovarian maturation, it was the first report of co-transcriptional repression of Liv-SGP-G using dsRNAs for homologous genes. This study indicates that subadult shrimp can respond to transcriptional silencing techniques.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Fisheries
Ratiporn Thongsoi, Siriluk Maskaew, Panumas Puechpon, Benjaporn Noppradit, Napassawan Inaek, Prapaporn Utarabhand, Phanthipha Runsaeng
Summary: In this study, a PPAE gene called FmPPAE1 was cloned and analyzed in banana shrimp. FmPPAE1 plays a vital role in regulating the proPO system, exhibits antibacterial properties, and contributes to the suppression of bacterial proliferation.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)
Article
Fisheries
Jishuang Tan, Paizelaiti Sahaer, Huan Li, Wei Han, Hongyan Sun
Summary: Circular RNAs (circRNA) are involved in gene expression and affect cellular processes such as transcription, splicing, and translation by interacting with microRNA and RNA binding proteins. A study identified circDNAJB6 as a circular RNA abundant in chicken macrophages after bacterial infection, and found that circDNAJB6 exacerbates cellular injury and interacts with multiple miRNAs and target genes, potentially impacting autophagy and MAPK signaling pathways.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)
Article
Fisheries
Rodrigo Guabiraba, Damaris Ribeiro Rodrigues, Paul T. Manna, Melanie Chollot, Vincent Saint-Martin, Sascha Trapp, Marisa Oliveira, Clare E. Bryant, Brian J. Ferguson
Summary: The innate immune response relies on the ability of host cells to detect and respond to microbial nucleic acids. Toll-like receptors (TLRs) play a crucial role in this process by distinguishing self from non-self. This study focused on TLR21, an avian TLR that recognizes bacterial DNA motifs. The findings suggest that avian TLR21 shares similar activation mechanisms to mammalian TLR9, highlighting the conservation of nucleic acid sensing mechanisms across species.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)
Article
Fisheries
Safieh Zeinali, Kate Sutton, Lonneke Vervelde
Summary: Chickens have a unique immune structure characterized by the absence of draining lymph nodes and the presence of well-developed mucosa-associated lymphoid tissue. The structure and development of chicken lymphoid tissues in the intestine, particularly Peyer's patches, have been poorly studied. However, the use of CSF1R-eGFP reporter transgenic chickens has allowed for the visualization and investigation of the development and distribution of these tissues. This research provides insights that could benefit mucosal vaccination strategies and the development of targeted vaccine delivery systems.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)
Retraction
Fisheries
G. -g. Li, Z. -z. Guo, X. -f. Ma, N. Cao, S. -n. Geng, Y. -q. Zheng, M. -j. Meng, H. -h. Lin, G. Han, G. -j. Du
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)
Article
Fisheries
Jinyue Yang, Peng Zhou, Wanrong Wu, Qingxiang Zhang, Dong Chen, Rui Luo
Summary: This study cloned and characterized the full-length sequence of duck MRE11 (duMRE11) for the first time, and found that this protein plays an important role in the innate immune response of ducks.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)
Article
Fisheries
Mengjiao Guo, Jiaqi Zhang, Mingtao Li, Xiaorong Zhang, Yantao Wu
Summary: rNLRC3 negatively regulates the NF -KB signaling pathway induced by P. multocida in rabbits by inhibiting the activation of NF -KB, reducing the expression of inflammatory cytokines, and interacting with rTRAF4 and rTRAF6. The NACHT-LRR domain is the functional domain of rNLRC3. These findings provide insights into the important role of rNLRC3 in combating P. multocida infection.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)
Article
Fisheries
Yinan Li, Xingye Lian, Wenwen Yang, Jinyuan Leng, Jiejie Sun, Yu Liu, Siqi Fan, Lingling Wang, Linsheng Song
Summary: The DM9-containing protein CgDM9CP-7 plays an important role in the innate immune response of molluscs. It is constitutively expressed in various tissues and is up-regulated in haemocytes after stimulation by Vibrio splendidus and lipopolysaccharide (LPS). CgDM9CP-7 binds to various pathogens and exhibits antimicrobial activity, while also regulating the expression of CgMyD88 and CgIL-17.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)
Article
Fisheries
Xiuwen Bao, Haoran Song, Liying He, Yong Li, Shuqi Niu, Jinlin Guo
Summary: This study focuses on the interaction between Hepialus xiaojinensis larvae and Ophiocordyceps sinensis during the infection process. The results show that the infected third instar larvae enhance antioxidant defense ability, while the infected fourth instar larvae reduce this ability and allocate more energy to growth and development.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)