Article
Developmental Biology
Timothy J. Duerr, Eun Kyung Jeon, Kaylee M. Wells, Antonio Villanueva, Ashley W. Seifert, Catherine D. McCusker, James R. Monaghan
Summary: In this study, a transgenic line of axolotls expressing FUCCI was generated to investigate cell division and cell contribution in regeneration. The results showed widespread expression of FUCCI in developing and adult tissues, which was validated by DNA synthesis and mitosis phase markers. Spinal cord amputation was found to increase cell proliferation at a certain distance from the injury site, and multimodal staining provided cell type information for cycling cells.
Article
Multidisciplinary Sciences
Stefania Ippati, Yuanyuan Deng, Julia van der Hoven, Celine Heu, Annika van Hummel, Sook Wern Chua, Esmeralda Paric, Gabriella Chan, Astrid Feiten, Thomas Fath, Yazi D. Ke, Nikolas K. Haass, Lars M. Ittner
Summary: The study suggests that neurons may exhibit transient, self-limited cell cycle reentry activity, indicating a dynamic process in their postmitotic state; neurons undergoing cell cycle reentry activity during oligomeric amyloid-beta challenge can maintain and increase the cell cycle indicator signal, thus evading cell death.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Yuki Hitora, Ai Sejiyama, Koyo Honda, Yuji Ise, Fitje Losung, Remy E. P. Mangindaan, Sachiko Tsukamoto
Summary: Natural products play important roles in drug development, specifically in cancer biology and the development of antitumor agents. In this study, a new sesquiterpene quinone, neoisosmenospongine, was isolated from a marine sponge extract, along with four known compounds. These compounds were found to inhibit cell cycle progression at S/G2/M phases, demonstrating the effectiveness of fluorescent image-based high-content screening for isolating cell cycle inhibitors from natural resources.
BIOORGANIC & MEDICINAL CHEMISTRY
(2021)
Article
Biology
Alexander P. Browning, Jesse A. Sharp, Ryan J. Murphy, Gency Gunasingh, Brodie Lawson, Kevin Burrage, Nikolas K. Haass, Matthew Simpson
Summary: Tumour spheroids are common experimental models in vitro, closely mimicking avascular tumour growth. Research suggests that spheroids have a limiting structure and the mature structure is independent of seeding density. Additionally, comparing spheroid structure rather than size produces more accurate results.
Article
Radiology, Nuclear Medicine & Medical Imaging
Yun Lu, Adriana V. F. Massicano, Carlos A. Gallegos, Katherine A. Heinzman, Sean W. Parish, Jason M. Warram, Anna G. Sorace
Summary: This study evaluates the accuracy of the FUCCI system compared to [F-18]-FLT PET/CT and histology, and explores its potential application for assessing cancer cell proliferation.
MOLECULAR IMAGING AND BIOLOGY
(2022)
Article
Oncology
Marion Baniol, Francesca Murganti, Agata Smialowska, Joni Panula, Eniko Lazar, Viveka Brockman, Sarantis Giatrellis, Wouter Derks, Olaf Bergmann
Summary: This study utilized a transgenic mouse model with the FUCCI system to isolate and characterize cycling cardiomyocytes, as well as perform single-cell transcriptome analysis of cardiomyocytes at different developmental stages. The results showed that cardiomyocytes have the highest mitotic activity at birth, followed by a gradual decline during the neonatal period, with premature cell cycle exit at G1/S shortly after birth and delayed G1/S progression during endoreplication. Single-cell RNA-seq confirmed signaling pathways involved in cardiomyocyte proliferation and maturation-related transcriptional changes during postnatal development. The study generated transcriptional profiles specific to cell division and endoreplication in cardiomyocytes at different developmental stages, which may help identify genes important for adult cardiomyocyte proliferation and heart regeneration.
EXPERIMENTAL CELL RESEARCH
(2021)
Review
Chemistry, Analytical
Vinayak Juvekar, Hyo Won Lee, Dong Joon Lee, Hwan Myung Kim
Summary: Two-photon microscopy is an important tool for deep-tissue imaging, providing high-resolution fluorescence images beyond millimeter depths. Recent research has focused on developing innovative strategies and probes for quantitative analysis using two-photon fluorescence.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Patiwet Wuttisarnwattana, Brendan L. Eck, Madhusudhana Gargesha, David L. Wilson
Summary: In this study, a model was developed to determine the optimal slice thickness for detection of fluorescent cells or microspheres. The model suggests a value that provides near-ideal sensitivity while minimizing scan time. Results show that cryo-imaging can accurately quantify the distribution of fluorescent cells with high recovery ratios.
SCIENTIFIC REPORTS
(2023)
Article
Computer Science, Artificial Intelligence
Ho Young Jeong, Seokcheon Lee
Summary: Battery-powered drones offer a promising solution for fast, cost-effective, and eco-friendly delivery. The hybrid delivery system combining trucks and drones has emerged as an efficient approach. A recent study introduced the Drone Routing Problem with Truck (DRP-T), which uses drones as the primary delivery system and features launch locations separate from customer sites. This study proposes a computationally efficient heuristic algorithm to mitigate the complexity of DRP-T.
EXPERT SYSTEMS WITH APPLICATIONS
(2023)
Article
Multidisciplinary Sciences
Scott Curran, Gautam Dey, Paul Rees, Paul Nurse
Summary: This study provides a comprehensive analysis of the spatial and temporal dynamics of cell cycle regulators in the fission yeast Schizosaccharomyces pombe. It reveals that the mitotic B-type cyclin Cdc13 and the regulatory phosphatase Cdc25 exhibit concentration changes at the whole-cell level, with higher concentrations in the nucleus. Additionally, the major fission yeast cyclin-dependent kinase Cdc2, the CDK regulator Sucl, and the inhibitory kinase Weel also show increased nuclear accumulation. The study proposes a two-step process for the control of mitosis.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Ravi K. Gupta, Petra Mlcochova
Summary: SARS-CoV-2 infection triggers redistribution of cyclin D1 and D3 from the nucleus to the cytoplasm, followed by proteasomal degradation. Cyclin D3 interacts with SARS-CoV-2 proteins and affects the assembly and release of newly produced virions, highlighting the importance of understanding the virus-host interactions.
Article
Biochemistry & Molecular Biology
Jennifer Alisa Amrhein, Lena Marie Berger, Amelie Tjaden, Andreas Kraemer, Lewis Elson, Tuomas Tolvanen, Daniel Martinez-Molina, Astrid Kaiser, Manfred Schubert-Zsilavecz, Susanne Mueller, Stefan Knapp, Thomas Hanke
Summary: The PCTAIRE subfamily of CDKs, including CDK16, represents an understudied class of kinases with potential implications in various diseases. By optimizing the structure, a highly potent inhibitor 43d was identified, which selectively targets CDK16 and other members of the PCTAIRE and PFTAIRE families. Cellular assays and cell cycle analysis confirmed the inhibitory effect of 43d.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Amandeep Kaur, Liam D. Adair, Sarah R. Ball, Elizabeth J. New, Margaret Sunde
Summary: In this study, the researchers developed a fluorescent amyloid sensor called AmyBlink-1 and used it for super-resolution imaging of amyloid structures. They were able to achieve nanoscale imaging of different types of amyloid fibrils and quantify their morphological features.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Immunology
Eva Lukas, Thea Hogan, Cayman Williams, Benedict Seddon, Andrew J. Yates
Summary: The dynamics of cell populations are often studied using pulse-chase DNA labeling techniques combined with mathematical models. In this study, an alternative method using a cell fate-mapping mouse model is explored, in which dividing cells constitutively express a fluorescent protein. The lifespans and division rates of naive CD4 and CD8 T cells are measured using a pulse-chase approach with this reporter mouse system, and the estimates are consistent with other published methods. It is proposed that modeling the timecourses of labeled cell frequencies in both the population of interest and its precursor simultaneously can provide unbiased parameter estimates and full measures of uncertainty. Ki67 reporter mice offer a promising system for modeling cellular dynamics.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Oncology
Kei Takahashi, Ryo Tanabe, Shogo Ehata, Shimpei Kubota, Yasuyuki Morishita, Hiroki R. Ueda, Kohei Miyazono
Summary: Tissue-clearing technology combined with the Fucci reporter system visualized the cell cycles of primary and metastatic tumors, showing different fluorescence patterns of the Fucci reporter in tumor colonies among various organs and even within the same organs. The Fucci reporter cells were also used to evaluate the effects of anti-tumor drugs on cancer cells.
Article
Endocrinology & Metabolism
Atsuhiko Hikita, Tadahiro Iimura, Yusuke Oshima, Takashi Saitou, Shin Yamamoto, Takeshi Imamura
Article
Multidisciplinary Sciences
Ji-Won Lee, Akiyoshi Hoshino, Kazuki Inoue, Takashi Saitou, Shunsuke Uehara, Yasuhiro Kobayashi, Satoshi Ueha, Kouji Matsushima, Akira Yamaguchi, Yuuki Imai, Tadahiro Iimura
NATURE COMMUNICATIONS
(2017)
Review
Oncology
Takeshi Imamura, Takashi Saitou, Ryosuke Kawakami
Article
Multidisciplinary Sciences
Takashi Saitou, Hiroshi Kiyomatsu, Takeshi Imamura
SCIENTIFIC REPORTS
(2018)
Letter
Dermatology
Keiko Usui, Nanako Kadono, Yuki Furuichi, Keiichiro Shiraga, Takashi Saitou, Hiroshi Kawasaki, Kiminori Toyooka, Hiroomi Tamura, Akiharu Kubo, Masayuki Amagai, Takeshi Matsui
JOURNAL OF DERMATOLOGICAL SCIENCE
(2019)
Article
Multidisciplinary Sciences
Masaki Maruta, Toshiki Ochi, Kazushi Tanimoto, Hiroaki Asai, Takashi Saitou, Hiroshi Fujiwara, Takeshi Imamura, Katsuto Takenaka, Masaki Yasukawa
SCIENTIFIC REPORTS
(2019)
Article
Multidisciplinary Sciences
Takeshi Ieda, Hiroshi Tazawa, Hiroki Okabayashi, Shuya Yano, Kunitoshi Shigeyasu, Shinji Kuroda, Toshiaki Ohara, Kazuhiro Noma, Hiroyuki Kishimoto, Masahiko Nishizaki, Shunsuke Kagawa, Yasuhiro Shirakawa, Takashi Saitou, Takeshi Imamura, Toshiyoshi Fujiwara
SCIENTIFIC REPORTS
(2019)
Article
Multidisciplinary Sciences
Takao Watanabe, Hiroko Ninomiya, Takashi Saitou, Sota Takanezawa, Shin Yamamoto, Yusuke Imai, Osamu Yoshida, Ryosuke Kawakami, Masashi Hirooka, Masanori Abe, Takeshi Imamura, Yoichi Hiasa
SCIENTIFIC REPORTS
(2020)
Article
Multidisciplinary Sciences
Sota Takanezawa, Takashi Saitou, Takeshi Imamura
Summary: The use of two-photon excitation in light-sheet microscopy is limited by its narrow excitation range, but a new illumination optics proposed in this study aims to generate longer extent Bessel beams to address this limitation. A microscope constructed with this technique allows for long-term time-lapse observations and high-speed recording with similar to 2-3-μm axial resolution, expanding the applications of two-photon excitation in light-sheet microscopy.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Kana Kobayashi-Taguchi, Takashi Saitou, Yoshiaki Kamei, Akari Murakami, Kanako Nishiyama, Reina Aoki, Erina Kusakabe, Haruna Noda, Michiko Yamashita, Riko Kitazawa, Takeshi Imamura, Yasutsugu Takada
Summary: This study successfully differentiated fibroepithelial lesions using a combined technique of label-free imaging with multi-photon microscopy and artificial intelligence. Tissue sections were detected using multi-photon excited autofluorescence and second harmonic generation (SHG) signals, and a deep learning framework was used to automatically separate epithelial and stromal regions. This provides quantitative signatures for epithelial and stromal alterations in breast tissues.
Article
Biochemistry & Molecular Biology
Takashi Saitou, Takeshi Imamura
Summary: This study aimed to construct a light-sheet microscope with higher resolution imaging while maintaining a large field of view. They used a low magnification (16x) and high numerical aperture (0.8) objective to address potential illumination and detection mismatch. By applying a depth of focus extension method, they demonstrated that the extended DOF system combined with wide-field two-photon light-sheet microscopy offers a simple and easy setup for live imaging of large multicellular organisms with sub-cellular resolution.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Yoshiaki Sugihara, Naoto Inai, Masayasu Taki, Thomas Baumgartner, Ryosuke Kawakami, Takashi Saitou, Takeshi Imamura, Takeshi Yanai, Shigehiro Yamaguchi
Summary: The use of donor-pi-acceptor (D-pi-A) skeletons with amino and boryl moieties as the electron-donating and -accepting groups can produce fluorophores with red-shifted emissions. The introduction of a dithienophosphole P-oxide scaffold as an acceptor-spacer in the D-A-A pi-system results in near-infrared emission fluorophores with high fluorescence quantum yields. The boryl group plays a crucial role in generating intense emission by lowering the nonradiative decay rate constant through reducing non-adiabatic coupling in the internal conversion process.
Article
Cell & Tissue Engineering
Junichi Kushioka, Takashi Kaito, Rintaro Okada, Hiroyuki Ishiguro, Zeynep Bal, Joe Kodama, Ryota Chijimatsu, Melanie Pye, Masahiro Narimatsu, Jeffrey L. Wrana, Yasumichi Inoue, Hiroko Ninomiya, Shin Yamamoto, Takashi Saitou, Hideki Yoshikawa, Takeshi Imamura
Article
Medicine, General & Internal
Takashi Saitou, Sota Takanezawa, Hiroko Ninomiya, Takao Watanabe, Shin Yamamoto, Yoichi Hiasa, Takeshi Imamura
FRONTIERS IN MEDICINE
(2018)