期刊
DEVELOPMENT
卷 142, 期 20, 页码 3592-3600出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/dev.123018
关键词
SHG/THG; Live imaging; Multiphoton microscopy; Neural stem cell; Pallium; Zebrafish
资金
- French Agence Nationale de la Recherche [ANR-08-CEX-08-000-01, ANR-2012-BSV4-0004-01, ANR-2010-JCJC-1510-01, ANR-10-INBS-04, ANR-11-EQPX-0029]
- Ecole des Neurosciences de Paris
- FRM [FRP 'Equipe'] [DEQ20120323692]
- European Research Council [AdG 322936]
- Region Ile-de-France [C'Nano IdF]
Live imaging of adult neural stem cells (aNSCs) in vivo is a technical challenge in the vertebrate brain. Here, we achieve long-term imaging of the adult zebrafish telencephalic neurogenic niche and track a population of >1000 aNSCs over weeks, by taking advantage of fish transparency at near-infrared wavelengths and of intrinsic multiphoton landmarks. This methodology enables us to describe the frequency, distribution and modes of aNSCs divisions across the entire germinal zone of the adult pallium, and to highlight regional differences in these parameters.
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