Article
Genetics & Heredity
Agnes Thierry, Varun Khanna, Bernard Dujon
Summary: Novel, large-scale structural mutations were discovered in engineered Saccharomyces cerevisiae strains, including giant chromosomes, complex segmental rearrangements, and the formation of neochromosomes. These mutations involved short repeated sequences and novel junctions between unrelated sequences in the genome, leading to an increase in chromosome number and stability.
Article
Oncology
Koel Mukherjee, Debpali Sur, Abhijeet Singh, Sandhya Rai, Neeladrisingha Das, Rakshanya Sekar, Srinu Narindi, Vandana Kumar Dhingra, Bhinyaram Jat, K. V. Vinu Balraam, Satya Prakash Agarwal, Prabhat Kumar Mandal
Summary: Oral Squamous Cell Carcinoma (OSCC) is a highly aggressive malignancy with the key marker for disease prediction largely unknown. This study found extremely high L1 retrotransposon activity in post-operative oral cancer samples, especially in the expression levels of L1ORF2p protein.
Review
Plant Sciences
Yunus Emre Arvas, Sevgi Marakli, Yilmaz Kaya, Ruslan Kalendar
Summary: The use of molecular markers is crucial in various fields of molecular genetics, such as gene identification, plant breeding, and genetic characterization. Transposable elements, particularly retrotransposons, are widely used as molecular markers due to their presence in eukaryotic genomes and ability to integrate into dispersed chromosomal locations. The ongoing development of molecular marker technologies is closely linked to high-throughput genotype sequencing platforms, making this research highly significant.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biochemical Research Methods
Fang Wang, Chang Li, Ruotong Zhang, Yuan Liu, Haisong Lin, Lang Nan, Muhammad Ajmal Khan, Yang Xiao, Ho Cheung Shum, Hui Deng
Summary: Cold atmospheric plasma chip enables targeted and multi-dosage treatment of multiple cell lines in a single session. This innovative approach provides a new tool for investigating the mechanisms of plasma-induced anticancer effects and guiding clinical applications.
Article
Plant Sciences
Stefan Cerbin, Shujun Ou, Yang Li, Yanni Sun, Ning Jiang
Summary: The genome of the sacred lotus contains a high abundance of transposable elements (TEs), with hAT (Ac/Ds) elements being the most prevalent. The presence of non-canonical LTR retrotransposons in such abundance is unprecedented. TEs are particularly enriched in genic regions, leading to significant expansion of intron sizes. Some genes show lower association with TEs, while others are enriched with them, particularly those involved in epigenetic pathways.
Article
Pharmacology & Pharmacy
Linhua Wei, Weiwei Chen, Linke Huang, Hui Wang, Yuangang Su, Jiamin Liang, Haoyu Lian, Jiake Xu, Jinmin Zhao, Qian Liu
Summary: This study explored the effects and mechanism of Alpinetin (Alp) on inflammatory osteolysis. The results showed that Alp suppressed the differentiation and function of osteoclasts, reduced reactive oxygen species production, and inhibited the expression of inflammatory genes. Moreover, Alp inhibited the phosphorylation of specific pathways and minimized LPS-induced osteolysis in mice.
PHARMACOLOGICAL RESEARCH
(2022)
Article
Virology
Yan Li, Jiaxin Yang, Siyu Shen, Wei Wang, Nian Liu, Haoran Guo, Wei Wei
Summary: The viral proteins encoded by SARS-CoV-2 can suppress the activity of L1 retrotransposons in host cells. These viral inhibitors modulate the transposition activity of L1 through a complex network, affecting host genome stability and antiviral immune activation.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Article
Agriculture, Multidisciplinary
Jialong Chen, Mingque Li, Xueqiong Zhou, Ailun Xie, Ziwei Cai, Chunlai Fu, Yongming Peng, He Zhang, Linhua Liu
Summary: The study revealed a novel feedback mechanism of p38-Parkin-ROS regulating rotenone-induced Parkinson's disease. Both p38 activation and Parkin inactivation contribute to the disruption of Parkin-mediated mitophagy, leading to increased reactive oxygen species (ROS) production. Inhibiting p38 and enhancing Parkin activity can reduce ROS levels and restore cell death and mitochondrial function in rotenone-induced PD models.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Microbiology
Zixiong Zhang, Ning Zhang, Saisai Guo, Qian Liu, Shujie Wang, Ao Zhang, Dongrong Yi, Jianyuan Zhao, Quanjie Li, Jing Wang, Yongxin Zhang, Ling Ma, Jiwei Ding, Shan Cen, Xiaoyu Li
Summary: In this study, it was found that the CCHC-type zinc-finger protein ZCCHC3 can repress LINE-1 retrotransposition, and this activity is closely related to its zinc-finger domain. Further studies showed that ZCCHC3 can post-transcriptionally diminish the LINE-1 RNA level. These findings demonstrate the significance of ZCCHC3 in the cellular control of LINE-1 replication.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Christopher H. Switzer, Hyun-Ju Cho, Thomas R. Eykyn, Paul Lavender, Philip Eaton
Summary: Inducible nitric oxide synthase (NOS2) and nitric oxide (NO) signaling lead to DNA hypomethylation and genomic instability through degradation of DNMT1 protein and retrotransposon activation. NOS2 expression levels are correlated with decreased DNA methylation and malignant cellular transformation in breast cancer patients.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Food Science & Technology
Ling Yang, Liping Jiang, Xiance Sun, Jing Li, Ningning Wang, Xiaofang Liu, Xiaofeng Yao, Cong Zhang, Haoyuan Deng, Shaopeng Wang, Guang Yang
Summary: Exposure to DEHP has been associated with reproductive toxicity, potentially through the upregulation of p38 alpha, p53, SAT1, ALOX15, and lipid ROS, forming a vicious cycle. This study provides new insights into the molecular mechanisms underlying DEHP-induced testicular toxicity.
FOOD AND CHEMICAL TOXICOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Ming-Fang Wu, Yen-Hsiang Huang, Ling-Yen Chiu, Shur-Hueih Cherng, Gwo-Tarng Sheu, Tsung-Ying Yang
Summary: Curcumin induces apoptosis in chemoresistant lung cancer cells through generation of ROS, and p38 MAPK is identified as a crucial signaling pathway for this process.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Yin Shi, Shengfeng Xu, Natalie Y. L. Ngoi, Qi Zeng, Zu Ye
Summary: Hypoxia in the tumor microenvironment, causing excessive ROS and genomic instability, is a hallmark of cancer contributing to self-renewal, metastasis, and therapy resistance. PRL-3, an oncoprotein, has been found to regulate apoptosis resistance by negatively affecting p38 MAPK activity in response to hypoxia stress.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
S. C. Broome, T. Pham, A. J. Braakhuis, R. Narang, H. W. Wang, A. J. R. Hickey, C. J. Mitchell, T. L. Merry
Summary: The role of mitochondrial ROS in muscle adaptations to exercise training was examined in this study. Supplementation with the mitochondria-targeted antioxidant MitoQ was found to enhance skeletal muscle PGC1 alpha mRNA expression following acute exercise. However, it had no significant effects on exercise-induced oxidative stress or training-induced mitochondrial content and function.
Article
Cell & Tissue Engineering
X. Wang, W. Jiang, K. Pan, L. Tao, Y. Zhu
Summary: This study confirms that melatonin promotes apoptosis of RAW264.7 cells through the BMAL1/ROS/MAPK-p38 pathway, and reveals the therapeutic effect and mechanism of melatonin in postmenopausal osteoporosis. This finding enriches BMAL1 as a potential target for the treatment of osteoporosis and the pathogenesis of postmenopausal osteoporosis.
BONE & JOINT RESEARCH
(2023)
Article
Plant Sciences
T. J. Zhang, L. J. Pan, Q. Huang, L. H. Zhu, N. Yang, C. L. Peng, L. B. Chen
Article
Biotechnology & Applied Microbiology
Dongsheng Zhang, Peng Hu, Taigang Liu, Jian Wang, Shouwen Jiang, Qianghua Xu, Liangbiao Chen
Article
Genetics & Heredity
Rizka Rahmana Putri, Liangbiao Chen
Article
Fisheries
Tao Zhou, Lang Gui, Mingli Liu, Wenhao Li, Peng Hu, Daniel F. C. Duarte, Hongbo Niu, Liangbiao Chen
FISH & SHELLFISH IMMUNOLOGY
(2019)
Article
Biology
Liangbiao Chen, Ying Lu, Wenhao Li, Yandong Ren, Mengchao Yu, Shouwen Jiang, Yanxia Fu, Jian Wang, Sihua Peng, Kevin T. Bilyk, Katherine R. Murphy, Xuan Zhuang, Mathias Hune, Wanying Zhai, Wen Wang, Qianghua Xu, Chi-Hing Christina Cheng
Article
Biology
Xuhui Han, Bingqi Wang, Dongxu Jin, Kuang Liu, Hongjie Wang, Liangbiao Chen, Yao Zu
Summary: Folic acid is an essential vitamin and its supplementation during pregnancy is crucial for embryonic development. This study demonstrates the impact of excessive or insufficient folic acid intake on cardiac development in zebrafish embryos and establishes a zebrafish mutant model for studying folate metabolism. The findings emphasize the importance of precise folic acid dosage for embryonic development.
Article
Genetics & Heredity
Qianqian Yan, Wenhao Li, Xiaoting Gong, Ruiqin Hu, Liangbiao Chen
Summary: In this study, GluK2 gene was found to be highly expressed in the brains and eyes of adult zebrafish and play a crucial role in cold stress. Gene knockout of GluK2 using the CRISPR/Cas9 system was performed, and differentially expressed genes related to the response to temperature stimulus pathway were identified through RNA-seq analysis. Behavioral phenotyping assay and TUNEL staining confirmed the necessity of GluK2 for cold tolerance in zebrafish.
Article
Fisheries
Yueyue Fei, Qin Wang, Jigang Lu, Linyue Ouyang, Wei Li, Ruiqin Hu, Liangbiao Chen
Summary: In this study, the researchers cloned the LEAP2 coding sequence from C. hamatus and found high levels of LEAP2 mRNA expression in the skin and liver. The mature peptide of LEAP2 demonstrated selective antimicrobial activities against various bacteria and showed bactericidal activity by destroying the cell membrane integrity and binding with bacterial genomic DNA. Overexpression of Tol-LEAP2-EGFP in zebrafish larva enhanced antimicrobial activity and reduced bacterial load and expression of pro-inflammatory factors. This research provides valuable insights into the antimicrobial activity of LEAP2 from C. hamatus and its potential role in enhancing resistance to pathogens.
JOURNAL OF FISH DISEASES
(2023)
Article
Environmental Sciences
Yihan Yu, Lihua Hu, Dandan Tian, Yingying Yu, Lingzheng Lu, Jiongming Zhang, Xianke Huang, Maocang Yan, Liangbiao Chen, Zhichao Wu, Wei Shi, Guangxu Liu
Summary: This study evaluated the effects of microplastics (MPs) and hexabromocyclododecane (HBCD), alone or in combination, on the hepatopancreas of whiteleg shrimp. The results showed evident histological injury, elevation in hepatic lesion markers, disruption of metabolism and detoxification processes, alterations in antioxidant enzymes, and oxidative damage to the hepatopancreas. Furthermore, accumulation of HBCD in shrimp and increased toxicity of HBCD-MP coexposure were observed.
ENVIRONMENTAL POLLUTION
(2023)
Article
Fisheries
Yan Zhou, Yanjie Zhang, Shang Wei, Wei Li, Wenhao Li, Zhichao Wu, Shouwen Jiang, Ying Lu, Qianghua Xu, Liangbiao Chen
Summary: This study found that long-term exposure to high temperatures has detrimental effects on the survival and metabolism of Nile tilapia. Fish reared at 36 degrees C showed significantly reduced survival rates and tolerance to hypoxia, as well as increased respiratory rates. After 15 weeks at 36 degrees C, a partial temperature compensation for routine metabolic rates was observed.
Article
Fisheries
Tao Zhou, Zhihua Fang, Daniel F. C. Duarte, Stefan A. Fernandes, Ying Lu, Jing Guo, Lang Gui, Liangbiao Chen
Summary: This study investigated the mechanisms of gill damage in tilapia infected with Streptococcus agalactiae. Transcriptomic analysis revealed differentially expressed genes and enriched immune-related gene ontology and pathways. HE staining results showed severe gill damage in tilapia at 9 hours post-infection.
Article
Fisheries
Carmen S. V. Sousa, Deborah M. Power, Pedro M. Guerreiro, Bruno Louro, Liangbiao Chen, Adelino V. M. Canario
Summary: The environmental conditions and isolation in the Antarctic have driven the evolution of a unique biodiversity. This study investigates the possible adaptation of the Notothenia coriiceps immune system to the cold environment and unique microbial community of the Southern Ocean. The findings suggest that the immune processes in N. coriiceps differ from other teleost fish.
Article
Fisheries
Shuang Han, Shang Wei, Ruoyu Chen, Man Ni, Liangbiao Chen
Summary: This study provides a model for understanding the response mechanisms of fish under cold stress by studying the physiological and behavioral changes in low-temperature domesticated fish. The results show that cold-tolerant fugu reduces brain tissue damage and copes with cold environment by employing a dynamic metabolic regulation strategy.
Article
Biochemistry & Molecular Biology
Mengchao Yu, Dongsheng Zhang, Peng Hu, Sihua Peng, Weiwen Li, Shunping He, Wanying Zhai, Qianghua Xu, Liangbiao Chen
BIOCHEMICAL SYSTEMATICS AND ECOLOGY
(2017)