Article
Food Science & Technology
Yi Liu, Shu Kang, Haijuan Zhang, Yi Kai, Hongshun Yang
Summary: This study investigated the effects of fish gelatin/chitosan-based films incorporated with lemon essential oil on grass carp fillets during chilled and iced storage. The results showed that the active films inhibited moisture transformation and improved the water-holding capacity of the fish samples. Additionally, the films effectively reduced the growth of bacteria and preserved the freshness of the samples. The combination of the active films and iced storage provided a promising strategy to prolong the shelf-life of grass carp fillets.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2023)
Article
Chemistry, Applied
Shuai Zhuang, Xiaochang Liu, Yan Li, Longteng Zhang, Hui Hong, Jun Liu, Yongkang Luo
Summary: The study revealed the deamination and decarboxylation activities of spoilage microbiota in chill-stored grass carp fillets. It was found that different taste amino acids were affected differently by microbial deamination activities. Additionally, putrescine in fish fillets was mainly produced through microbial decarboxylation activity of ornithine, and spoilage microbiota showed active glucose utilization during the late period of storage.
Article
Immunology
Jiancheng Zhou, Mengzhen Feng, Weixiang Zhang, Rui Kuang, Qi Zou, Jianguo Su, Gailing Yuan
Summary: Intensive high-density fish farming is leading to increasing bacterial diseases. Hepcidin plays a role in innate immunity and iron metabolism during bacterial infection. This study investigated the effect of hepcidin and chitosan combination against Flavobacterium columnare. The optimal dosing regimen was found to be every three days. The combination of hepcidin and chitosan improved grass carp growth, immunity, and gut microbiota, and protected against bacterial infection.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Fisheries
Zhensheng Wang, Xingchen Huo, Yanqi Zhang, Yang Gao, Jianguo Su
Summary: This study demonstrates that nanoparticle CMCS-20a loaded with carboxymethyl chitosan (CMCS) has a good preventive effect on bacterial diseases in aquaculture. CMCS-20a can effectively kill bacteria and reduce the influence of external factors, it is almost non-toxic to fish cells, and can increase the activity of important antibacterial substances in the serum. Furthermore, CMCS-20a can improve fish survival rate by regulating inflammation responses, reducing bacterial burdens, and alleviating tissue lesions.
Article
Fisheries
Kai Hao, Yu Wang, Bin Zhu, Fei Yu, Zhe Zhao, Gao-Xue Wang
Summary: This study demonstrates that surface display of GCRV core antigen vaccine and invasion component from aquatic pathogenic microorganism on nonpathogenic Escherichia coli can effectively prevent GCRV viral diseases via the immersion administration approach.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Food Science & Technology
Ramazan Sevik, Ayse Janseli Denizkara, Gokhan Akarca, Azize Atik, Ilker Atik
Summary: This study investigated the effects of rosemary and laurel essential oils added to marination formulas of common carp. The results showed that this marination process positively affected the microbiological and physicochemical properties of the fish fillets.
JOURNAL OF AQUATIC FOOD PRODUCT TECHNOLOGY
(2022)
Article
Agriculture, Multidisciplinary
Jiandong Shen, Wei Zhang, Qixing Jiang, Pei Gao, Yanshun Xu, Wenshui Xia
Summary: The study found that cysteine proteinase activity increased in chilled grass carp fillets, leading to a decline in shear force and an increase in drip loss. The purified 29 kDa cysteine proteinase was identified as cathepsin L, which caused collagen fibers to partly disintegrate. Release of soluble proteins and glycosaminoglycans from cathepsin L-treated collagen fibers was time dependent, with a slight release of hydroxyproline. Furthermore, cathepsin L was found to inhibit the destruction of intramuscular connective tissues.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2022)
Article
Fisheries
Yuting Qin, Zhaosheng Sun, Wei Wang, Jiawen Xu, Bangjie Wang, Zhao Jia, Xia Li, Junya Wang, Qian Gao, Xinhua Chen, Jun Zou
Summary: CD3 is a crucial component of the TCR-CD3 complex in adaptive immunity, with its homologues CiCD3γ/δ and CiCD3ε identified in grass carp and shown to be highly expressed in healthy fish and modulated by infections. A monoclonal antibody against CiCD3γ/δ was successfully produced, which showed good reactivity in Western blotting and flow cytometry analysis. The CiCD3γ/δ(+) cells were found to be localized in the gills and head kidney, accounting for a small percentage of lymphocytes in different organs.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2021)
Article
Fisheries
Shanshan Zeng, Meifeng Li, Xining Cheng, Shina Lu, Zhiqing Feng, Zeyin Jiang, Zhichao Sun, Xiaowen Xu, Huiling Mao, Chengyu Hu
Summary: In this study, it was found that grass carp DYRK2 induces cell apoptosis by increasing the phosphorylation level of p53.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Chemistry, Applied
Xiao-Qing Liu, Lin Feng, Pei Wu, Yang Liu, Hong-Mei Ren, Xiao-Wan Jin, Sheng-Yao Kuang, Shu-Wei Li, Ling Tang, Lu Zhang, Hai-Feng Mi, Xiao-Qiu Zhou, Wei-Dan Jiang
Summary: The study found that adding 6-9g/kg of CLA in the grass carp diet can increase muscle protein and fatty acid content, improve the physical properties of muscles, and ultimately improve the flesh quality of sub-adult grass carp.
Article
Biotechnology & Applied Microbiology
Huiqin Niu, Yifan Pang, Lingli Xie, Qiaozhen Yu, Yubang Shen, Jiale Li, Xiaoyan Xu
Summary: This study investigates the evolutionary background and expression regulation of grass carp miRNAs. Multiple novel miRNAs have emerged during grass carp evolution, and both duplication and de novo production contribute to miRNA cluster formation, with de novo creation being more prevalent. Clustered miRNAs are more conserved and change slower, while unique miRNAs tend to have higher evolution rates and lower expression levels.
Article
Virology
Ling Yang, Jianguo Su
Summary: In this study, it was found that GCRV can only infect leukocytes in vitro, while erythrocytes and thrombocytes are not susceptible. Additionally, the expression levels of immune response-related genes were significantly up-regulated in infected leukocytes.
Article
Food Science & Technology
Jixuan Liu, Fang Yang, Pei Gao, Dawei Yu, Peipei Yu, Qixing Jiang, Yanshun Xu, Wenshui Xia
Summary: The study determined that intramuscular connective tissue (IMCT) plays an important role in softening of ice-stored grass carp fillet, with its destruction leading to changes in fish texture. Important proteins in IMCT, such as decorin, elastin, and collagen, decreased during storage, particularly in the early stages.
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Immunology
Meifeng Li, Jihuan Hu, Huiling Mao, Dongming Li, Zeyin Jiang, Zhichao Sun, Tingting Yu, Chengyu Hu, Xiaowen Xu
Summary: In this study, it was found that grass carp KAT8 blocked the activities of IRF3 and IRF7 by acetylating them, resulting in the inhibition of nucleic acids-induced innate immune response.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Fisheries
Hongyan Yu, Xuewen Jia, Yifan Pang, Huiqin Niu, Biao Du, Xiaoyan Xu, Jiale Li
Summary: In this study, it was found that the TBK1 gene in grass carp could be upregulated by bacterial infection. Overexpression of TBK1 could reduce the number of adhesive bacteria in cells and promote cellular migration, proliferation, vitality, and anti-apoptosis ability. TBK1 could activate the NF-kappa B signaling pathway and induce autophagy in cells. These findings provide important information about the defense and immune mechanisms used by grass carp against pathogens.
FISH & SHELLFISH IMMUNOLOGY
(2023)
