Article
Agriculture, Multidisciplinary
Svenja Schneider, Simon Hammann, Heiko Hayen
Summary: A comprehensive analysis of polar lipids in whole grain wheat and oat was conducted, resulting in the identification of 67 lipid species belonging to nine polar lipid classes. The relative composition of four specific lipid classes was investigated in detail.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Review
Chemistry, Analytical
Qianying Sheng, Meiyan Liu, Minbo Lan, Guangyan Qing
Summary: Hydrophilic interaction liquid chromatography (HILIC) is an attractive complementary method to reversed phase LC for the separation of hydrophilic or strongly polar substances. The advancements in HILIC have significantly improved sensitivity, analyte coverage, throughput, analysis speed, and resolution in bio-separation and bio-analytical chemistry.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Stefano Dugheri, Nicola Mucci, Donato Squillaci, Elisabetta Bucaletti, Giovanni Cappelli, Lucia Trevisani, Cecile Valsecchi, Viviana Consonni, Fabio Gosetti, Davide Ballabio, Giulio Arcangeli
Summary: Antineoplastic drugs, known for their severe toxic effects, require sensitive monitoring methods to address surface contaminations within preparation and administration units. An orthogonal normal phase analysis method has been developed to complement the limitations of the more common reversed-phase methods, and has shown promise in reliable relationship evaluation between chromatographic retention and chemical-structural features of the drugs under analysis.
Article
Biochemical Research Methods
Xiaoqing Wang, Jian Cui, Jin Zhou, Shuo Wang, Yiming Gu, Xiaowei Liu, Shudong Wang
Summary: Vinyl modified silica was synthesized through a silane coupling reaction and a poly-acrylamide modified silica (PAM-SIL) stationary phase was prepared via free radical polymerization. The separation performance of the stationary phases could be regulated by controlling the polymer structure, showing potential for future separation and analysis applications.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Biochemical Research Methods
Bo Yuan, Weiting Lyu, Fekadu F. Dinssa, James E. Simon, Qingli Wu
Summary: A hydrophilic interaction ultra-high performance liquid chromatography with triple quadrupole tandem mass spectrometry method was developed and validated for the quantification of 21 free amino acids, showing improved sensitivity, wide linear range, and fast sample testing speed. The use of ammonium formate and hydrochloric acid were optimized to enhance sensitivity and response linearity for basic amino acids.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Biochemical Research Methods
Dimitrios J. Floros, Kangkang Xu, Franz Berthiller, Heidi Schwartz-Zimmermann
Summary: Metabolomics is gaining popularity in livestock research, and this study compared different chromatographic methods to assess their coverage of mammalian metabolites. The study investigated 354 primary metabolites from various compound classes and optimized selected reaction monitoring transitions on a mass spectrometer. The results showed that hydrophilic interaction liquid chromatography (HILIC) methods had the highest coverage of metabolites, including polar ones such as amino acids and biogenic amines. Combining complementary HILIC and reversed-phase (RP) methods increased the coverage to 92%, and further incorporating anion exchange chromatography (AIC) achieved a coverage of 97%.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Biochemical Research Methods
Xuan Zhang, Yunhua Hui, Mei Jiang, Youqiong Cai, Dongmei Huang, Guangxin Yang, Cong Kong
Summary: A rapid and simple method for the determination of 6 biogenic amines in food was established on HPLC-MS/MS without derivatization, showing good linearity, high correlation coefficient, and low detection limits. The method demonstrated good recoveries and stability, making it suitable for monitoring biogenic amines in various food products.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Biochemical Research Methods
Oluwatosin Daramola, Cristian D. Gutierrez-Reyes, Junyao Wang, Judith Nwaiwu, Sherifdeen Onigbinde, Mojibola Fowowe, Michael Dominguez, Yehia Mechref
Summary: Changes in glycan expression play a role in the development of diseases. Analyzing isomeric N-glycans using LC-MS/MS is challenging due to the large number of possible conformations. Derivatization of glycans is commonly used but time-consuming. This study focuses on efficient isomeric separation of native N-glycans using a nano ZIC-HILIC column. The stability and reproducibility of the column were confirmed.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Biochemical Research Methods
Haibin Li, Chusheng Liu, Li Zhao, Dongsheng Xu, Tingting Zhang, Qiqin Wang, Deirdre Cabooter, Zhengjin Jiang
Summary: An unmatched or improperly matched sample solvent can distort the peak shape of analytes in chromatography. For retained compounds, pure ACN is the best sample solvent, while for compounds eluting close to the solvent peak, distortions occur with pure ACN. Water in the sample solvent favors electrostatic attraction for charged compounds, leading to increased retention time.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Biochemical Research Methods
Lili Guo, Shruti Nayak, Yuan Mao, Ning Li
Summary: Adding glycine to mobile phases during HILIC-ESI-MS analysis significantly enhances the sensitivity of mass spectrometry detection, increases ion intensities, improves tandem MS spectral quality of N- and O-glycans, and does not affect chromatographic performance.
ANALYTICAL BIOCHEMISTRY
(2021)
Article
Biochemical Research Methods
Franziska Wienberg, Marcel Hoevels, Konrad Kosciow, Uwe Deppenmeier
Summary: The article discusses the rapid development of strategies to improve human health by modulating the gut microbiota, with a focus on the use of prebiotics. A method using inulosucrase from Lactobacillus for the synthesis of inulin-FOS was studied, along with the development of a time-saving and resource-efficient HPLC method for rapid analysis of these oligosaccharides.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2021)
Article
Biochemical Research Methods
Dana Moravcova, Richard Cmelik, Jana Krenkova
Summary: This study demonstrates the advantages of using mixtures of organic solvents for the separation of labeled oligosaccharides under hydrophilic interaction liquid chromatography conditions. Successful separation of labeled isomeric trisaccharides was achieved, and adjusting the volume ratio of solvents allowed for rapid separation of different labeled saccharides.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Chemistry, Analytical
Fei Wang, Fan Yang, Jiawei Liu, Quan Bai
Summary: This study investigates the retention behavior and mechanism of solutes in hydrophilic interaction chromatography (HILIC) using stoichiometric displacement theory (SDT). A HILIC/reversed-phase liquid chromatography (RPLC) dual-retention mechanism is studied in detail using a β-CD HILIC column. The study finds that a four-parameter equation derived from SDT-R accurately describes the U-shaped curves of solutes with RPLC/HILIC dual-retention mechanisms on the β-CD column, providing a theoretical guide for the development of HILIC and the enhancement of separation efficiency.
Article
Chemistry, Analytical
Isabelle Kohler, Michel Verhoeven, Rob Haselberg, Andrea F. G. Gargano
Summary: Proteomics and metabolomics have become increasingly important in clinical applications, with RPLC-MS and HILIC-MS techniques playing crucial roles in biomarker discovery and disease diagnosis. While RPLC-MS and HILIC-MS complement each other in resolving the complexity of proteome and metabolites, they also have technical limitations and potential solutions that need to be addressed.
MICROCHEMICAL JOURNAL
(2022)
Article
Biochemical Research Methods
Min Su, Kristian Serafimov, Peng Li, Cornelius Knappe, Michael Laemmerhofer
Summary: The chromatographic behavior of mixed-mode and hydrophilic interaction liquid chromatography (HILIC) was assessed in this study for the separation of metabolites from glycolysis and pentose phosphate pathways. Optimization of chromatographic conditions allowed for selective targeted analysis of glycolytic phosphorylated carbohydrate metabolites using liquid chromatography with tandem mass spectrometry (MS/MS). The separation of pentose phosphate isomers was achieved using mixed-mode HILIC/SAX and HILIC BEH Amide columns. HILIC-MS/MS showed challenges in separating hexose monophosphate metabolites, but the BEH Amide column provided the best results for this separation.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Chemistry, Applied
Ziba Hosseinpour, Vida Rezaei
Summary: Sol-gel chemistry is a unique method for constructing high-performance flexible sensors. This study successfully developed a novel naked eye sensor for fast detection of oxalate by incorporating indole inside the pores of sol-gel silica glasses. The sol-gel films exhibited excellent sensitivity, leaching resistance, and response time under optimized conditions. The prepared sensor showed a wide detection range and low detection limit for oxalate, and displayed good precision for both inter-day and intra-day measurements.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2024)
Article
Chemistry, Applied
S. T. Sarhir, R. Belkhou, A. Bouseta, A. A. Hayaloglu
Summary: In order to optimize the quality of Lben, this study investigated the impact of starter, fermentation conditions and substrate composition on its organoleptic and technological characteristics using response surface methodology. An optimized model for Lben production was developed based on fermentation time, Lben sensory quality and its biological activities.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2024)
Article
Chemistry, Applied
Kornelia Kadac-Czapska, Malgorzata Rutkowska, Eliza Knez, Piotr Konieczka, Malgorzata Grembecka
Summary: The quality of food is crucial for the healthy growth of children, and this study investigated the levels of mercury in infant formulas. The analysis revealed significant variations in mercury levels among different products. While the current assessment suggests mercury content is within acceptable limits, elevated levels in certain products may pose health risks.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2024)
Article
Chemistry, Applied
Velisaria-Eleni Gerogianni, Eirini A. Panagopoulou, Paraskevi B. Vasilakopoulou, Vaios T. Karathanos, Antonia Chiou
Summary: This study evaluated the content of free and bound polar phenols in Corinthian currant and identified phenolic acids and flavonoids as components of its bound polar phenol fraction. An efficient method for the release of bound phenols from the fruit matrix was also applied. This research has significant implications for understanding the health benefits of Corinthian currant.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2024)
Article
Chemistry, Applied
Jose Eduardo de Matos Paz, Aline Macedo Dantas, David Douglas de Sousa Fernandes, Marcio Jose Coelho Pontes
Summary: This work presents a new methodology based on digital images and multivariate analysis for the classification of sugar samples based on their color. By analyzing the frequency distribution of color indices and developing classification models, high accuracy can be achieved. The proposed method offers advantages such as low cost, simplicity, and non-destructiveness.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2024)
Article
Chemistry, Applied
Maria Guerrero-Chanivet, Manuel J. Valcarcel-Munoz, M. Valme Garcia-Moreno, M. Carmen Rodriguez-Dodero, Dominico A. Guillen-Sanchez
Summary: This study conducted a comprehensive analysis of Brandies de Jerez aged in Sherry Casks with different seasoning times and types, demonstrating significant differences in chemical composition and sensory characteristics among the brandies.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2024)
Article
Chemistry, Applied
Chayuttapong Malai, Kanvisit Maraphum, Khwantri Saengprachatanarug, Seree Wongpichet, Arthit Phuphaphud, Jetsada Posom
Summary: The quality of cassava can be measured using NIR spectroscopy to determine the starch content and dry matter content. A model developed from different scanning methods and surface conditions was compared, and the accuracy of the model using selected wavelengths was assessed. The prediction accuracy was found to depend on the scanning method, with the horizontal spectra obtaining the highest accuracy. The CARS-PLSR method was considered appropriate for predicting starch content, and the BOSS-PLSR method for predicting dry matter content.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2024)
Article
Chemistry, Applied
Abhishek Sharma, Atul Kumar
Summary: This study detected antibiotics residues in goat milk in the Western Himalayan region of India and assessed the related health risks. It was found that the contamination did not pose acute health risks to adults and children at current levels, but children may be at a greater risk. The study highlights the need for monitoring and education to protect human health.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2024)
Article
Chemistry, Applied
Livya Alves Oliveira, Stephanie Michelin Santana Pereira, Kelly Aparecida Dias, Stefany da Silva Paes, Mariana Grancieri, Luis Gonzalo Salinas Jimenez, Carlos Wanderlei Piler de Carvalho, Eugenio Eduardo de Oliveira, Hercia Stampini Duarte Martino, Ceres Mattos Della Lucia
Summary: This study characterized the powders of Tenebrio molitor and Gryllus assimilis, two types of insects, at different developmental stages. The powders were found to be rich in proteins, minerals, and vitamins, and showed good digestibility and solubility. The developmental stage of the insects affected their nutritional composition and functional properties.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2024)
Article
Chemistry, Applied
Fernanda Rodrigues Spinelli, Jucara Aparecida Andre, Paulo Gustavo Celso, Marlos Schuck Vicenzi, Eder Julio Kinast, Caren Regina Cavichioli Lamb, Glauco Bertoldo
Summary: The production, marketing and consumption of grape juices have been constantly increasing in recent years due to consumers' search for products with proven health benefits. Brazilian data shows that grape juice produced from V. labrusca L. and hybrids has a lower soluble solids content, with more than half of the production falling between 14 and 16 degrees Brix. This suggests the need to re-discuss the 16 degrees Brix limit established by CXS 247-2005.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2024)
Article
Chemistry, Applied
Fatima Zohra Issaad, Ala Abdessemed, Khalid Bouhedjar, Hani Bouyahmed, Mouna Derdour, Karima Ouffroukh, Ahmed Fellak, Mohamed Abd Salem Dems, Salah Chihoub, Radouane Bechlem, Abdelkader Mahrouk, Mourad Houasnia, Amine Belaidi, Khaled Moumed, Zohir Sebai, Faiza Saidani, Houria Akmouche
Summary: This study investigates the differences in physicochemical parameters and composition of monovarietal extra virgin olive oil from different varieties in Algeria, and applies machine learning techniques for classification and verification analysis.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2024)
Article
Chemistry, Applied
Seyedeh Faezeh Taghizadeh, Ramin Rezaee, Gholamreza Karimi
Summary: This study assessed the risk of simultaneous oral exposure to a combination of 20 pesticides and 7 metals in citrus fruit samples. The results showed that the oral exposure to non-carcinogenic metals did not pose a major risk, while nickel intake presented a moderate risk. The intake of organophosphorus compounds indicated a moderate risk, while no significant risk was found for pyrethroids.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2024)
Article
Chemistry, Applied
Jian Zhao, Min Guo, Patricia Martins, Joao Ramos, Lingxi Li, Baoshan Sun
Summary: This study analyzed the structural composition of eight-year-old Syrah red wines produced using different winemaking technologies. The results showed that the structural features of polymeric polyphenols, including the proportions of proanthocyanidins, amino acids, phenolic acids, and anthocyanins, as well as the mean degree of polymerization, varied significantly depending on the winemaking technology used. These findings are important for winemakers to control the polymeric polyphenol composition of wines.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2024)
Article
Chemistry, Applied
Mahunan Francois Assogba, Roger Houechene Ahouansou, N. Olive Ruth Djohi, Yenoukounme Euloge Kpoclou, Gildas Dona Anihouvi, Herbert Ogouyo Iko Af Afe, Biowa Franck Tchibozo, Caroline Douny, Jacques Mahillon, Marie -Louise Scippo, Djidjoho Joseph Hounhouigan, Victor Bienvenu Anihouvi
Summary: This study assessed the performance of new equipment and aimed to reduce the level of polycyclic aromatic hydrocarbons (PAHs) in the end products. The results showed that using QUALISANI equipment with charcoal achieved the highest thermal efficiency and the lowest PAH levels in compliance with standards.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2024)
Article
Chemistry, Applied
Mehmet Ali Saridas, Erdal Agcam, Nafiye Unal, Asiye Akyildiz, Sevgi Paydas Kargi
Summary: Turkey's geographical location and climate conditions are suitable for growing various fruit species, with apricots being the most famous. Local and foreign varieties differ significantly in terms of soluble solids and acid content. The low taste values in foreign varieties are mainly due to their genotypic structure and early harvest. There are also differences in the main sugars and acid content among different apricot varieties.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2024)