Article
Fisheries
Inacio Mateus Assane, Elielma Lima de Sousa, Gustavo Moraes Ramos Vallada, Geovana Dotta Tamashiro, Eduardo Criscoulo-Urbinati, Diogo Teruo Hashimoto, Fabiana Pilarski
Summary: This study investigated the characteristics of A. jandaei strains associated with massive mortalities in freshwater fish in Brazil, including morphology, biochemistry, molecular features, virulence, resistance, and pathogenicity. Variations were observed in virulence genes, experimental infections, and antimicrobial susceptibility among different strains.
Article
Fisheries
Hadeer A. Youssef, Hala F. Ayoub, Eman Soror, Aya F. Matter
Summary: This study developed and evaluated live and inactivated vaccines for Aeromonas veronii pathogenicity in Nile tilapia. The results showed that the live vaccine A(HY1) had the best protective efficacy against the highly pathogenic strain A(HY2).
AQUACULTURE INTERNATIONAL
(2023)
Article
Fisheries
Salah M. Aly, Mohamed E. Abou-El-Atta, Heba S. El-Mahallawy, Ahmed Elaswad, Fatma A. ElAbyad, Noha I. ElBanna
Summary: This study investigated the bacterial causes of ulcerative syndrome in Nile tilapia and the associated environmental stressors. Clinical, bacteriological, histopathological, and molecular techniques were used to identify bacteria (Aeromonas veronii biovar sobria and A. caviae), assess their antibiotic resistance, and analyze water quality parameters. Results indicated that water temperature and ammonia concentrations increased significantly in July and August, while pH levels were highest in August. Overall, improving water quality is important for maintaining the health of farmed fish.
AQUACULTURE INTERNATIONAL
(2023)
Article
Fisheries
Ying-da Wang, Jin-shuo Gong, Yong-chao Guan, Ze-lin Zhao, Ya-nan Cai, Xiao-feng Shan
Summary: In this study, mutant and complemented strains of A. veronii TH0426 were constructed to investigate the impact of the hcp1 gene on the bacterial virulence. Deletion of the hcp1 gene resulted in flagella fracture and affected basic biological characteristics and pathogenicity of TH0426 cells. The study demonstrates that the hcp1 gene regulates flagella assembly and plays a role in the animal pathogenesis of TH0426.
Article
Microbiology
Sandie Bispo dos Santos, Miguel Fernandez Alarcon, Anelise Stella Ballaben, Ricardo Harakava, Renata Galetti, Mateus Cardoso Guimaraes, Mariene Miyoko Natori, Leonardo Susumu Takahashi, Ricardo Ildefonso, Marco Rozas-Serri
Summary: Brazil, a major producer of Nile tilapia, is facing a major challenge of infectious diseases, with at least five new pathogens identified in the last five years. This study isolated and identified the causative agent of an epidemic outbreak in farmed Nile tilapia in Brazil, which was confirmed to be highly pathogenic and multidrug-resistant Aeromonas veronii. The bacteria showed susceptibility only to florfenicol and demonstrated high mortality rates in tilapia.
Article
Microbiology
Vera Irene Erickson, Ahmed Alfifi, Yaovi Gildas Mahuton Hounmanou, Mohammad Jafar Sana, Jens Peter Christensen, Anders Dalsgaard
Summary: A whole genomic analysis of 24 strains of Aeromonas veronii from a Danish abattoir revealed their widespread distribution in human, aquatic, and poultry samples, carrying various virulence factors and showing potential clinical and veterinary importance. Further epidemiological studies are needed to determine its pathogenicity in poultry.
VETERINARY MICROBIOLOGY
(2023)
Article
Fisheries
Aldo Aparecido Proietti-Junior, Luciana Sampaio Lima, Emily Moraes Roges, Yan Correa Rodrigues, Karla Valeria Batista Lima, Dalia Prazeres Rodrigues, Marcos Tavares-Dias
Summary: This study reported an outbreak of disease in pirarucu caused by Aeromonas, conducted experimental co-infection research, and characterized resistance profile, virulence factors, and phenotypic and molecular differentiation. The findings support the existence of synergism between A. hydrophila and A. jandaei in pirarucu, with implications for occurrences of this co-infection and determinants of virulence.
AQUACULTURE RESEARCH
(2021)
Article
Fisheries
Ruoming Li, Jiaxin Tian, Qiumei Shi, TongLei Wu, Dingjie An, Xiaofeng Shan, Guiqin Wang
Summary: The deletion of the cadA gene in A. veronii significantly decreased its virulence and adhesion while increasing biofilm formation ability.
AQUACULTURE REPORTS
(2021)
Article
Biotechnology & Applied Microbiology
Francis S. Legario, Casiano H. Choresca, Kathryn Grace, James F. Turnbull, Margaret Crumlish
Summary: This study characterized and profiled motile Aeromonas species recovered from sick tilapia farmed in the Philippines. The results showed that Aeromonas veronii is the main pathogen causing motile Aeromonas septicaemia in farmed tilapia, and it exhibits diverse genotypes, biochemical profiles, and antibiotic resistances.
JOURNAL OF APPLIED MICROBIOLOGY
(2023)
Article
Fisheries
Yasmine H. Tartor, El-Sayed Y. EL-Naenaeey, Hossam M. Abdallah, Mohamed Samir, Mayada M. Yassen, Ashraf M. Abdelwahab
Summary: This study focused on genotyping Aeromonas hydrophila isolated from diseased Nile tilapia, identifying virulence genes and resistance profiles. Results showed high prevalence of A. hydrophila in Nile tilapia with diverse antimicrobial resistance and virulence profiles. The study highlights the importance of monitoring and controlling MDR A. hydrophila in aquaculture.
Article
Microbiology
Ahmed H. Sherif, Amina S. Kassab
Summary: This study identified 18 different strains of Aeromonas hydrophila in Nile tilapia broodstock, with most of the strains being sensitive to ciprofloxacin and florfenicol and 8 strains showing multiple drug resistance. Additionally, the study found that titanium dioxide nanoparticles had a bactericidal effect on A. hydrophila.
Article
Microbiology
Hong Li, Xiang Ma, Yanqiong Tang, Dan Wang, Ziding Zhang, Zhu Liu
Summary: This study constructed a genome-wide PPI network for Aeromonas veronii and found that its virulence factors had higher degree and betweenness centrality, tended to interact with each other, and were enriched in specific modules. The virulence factors may regulate A. veronii's virulence through signal transduction pathways and manipulate host biological processes by mimicking and competitively binding to host proteins.
Article
Fisheries
Lei Zhang, Shengnan Jin, Chao Feng, Haichao Song, Sayed Haidar Abbas Raza, Huabo Yu, Liang Zhang, Teng Chi, Yanling Qi, Dongxing Zhang, Aidong Qian, Ning Liu, Xiaofeng Shan
Summary: This study constructed a mutant strain to investigate the functional role of aodp gene in A. veronii TH0426, revealing that aodp deficiency leads to changes in growth rate, drug resistance, swimming ability, pathogenicity, and caspase activity of the bacterium. The down-regulation of 17 genes related to virulence due to aodp deficiency provides insights for further studies on the pathogenesis of A. veronii.
JOURNAL OF FISH DISEASES
(2022)
Article
Immunology
Hong Wang, Ze Lin-Zhao, Ding Jie-An, Jing Lin-Wang, Bin Tong-Yang, Yuan Huan-Kang, Dong Xing-Zhang, Hai Chao-Song, Xiao Feng-Shan, Ai Dong-Qian
Summary: Aeromonas veronii (A. veronii) is a pathogen that can infect aquatic organisms and mammals. This study found that the mutant strain Delta lip led to a decrease in the adhesion and virulence of the wild-type strain.
MICROBIAL PATHOGENESIS
(2022)
Article
Fisheries
Nada M. A. Hashem, Mai A. M. El-Son, Ahmed Ateya, Rasha M. Saleh
Summary: This study investigated the effect of bovine lactoferrin dietary supplementation on haematological and immunological parameters, antioxidant genes, stress markers and disease resistance in Nile tilapia. The results showed that LF supplementation at doses of 800mg/kg or 1200mg/kg improved haematological and immunological parameters, and decreased stress markers, both before and after infection.
AQUACULTURE RESEARCH
(2022)
Article
Fisheries
Partho Pratim Debnath, Nguyen Dinh-Hung, Suwimon Taengphu, Vuong Viet Nguyen, Jerome Delamare-Deboutteville, Saengchan Senapin, Chadag Vishnumurthy Mohan, Ha Thanh Dong, Channarong Rodkhum
Summary: Sixteen countries have reported the presence of tilapia lake virus (TiLV), an emerging pathogen affecting tilapia. In Bangladesh, 20% of farms tested positive for TiLV in tilapia, while co-cultivated fish species and invertebrates did not show signs of TiLV infection. Experimental infection only showed clinical signs in Nile tilapia, suggesting that species in polycultures may not be susceptible to TiLV.
JOURNAL OF FISH DISEASES
(2022)
Article
Fisheries
Le Thanh Dien, Nguyen Vu Linh, Thao Thu Mai, Saengchan Senapin, Sophie St-Hilaire, Channarong Rodkhum, Ha Thanh Dong
Summary: Injection of ozone nanobubbles into water reduces bacterial load, improves dissolved oxygen, and modulates the fish innate immune system. This study investigates the effect of oxygen and ozone nanobubbles on a specific phage and finds that ozone nanobubbles can eradicate the phage while improving its adherence to fish body surfaces and penetration into internal organs. The use of ozone nanobubbles shows potential in reducing viral load in aquaculture.
Article
Fisheries
Todsaporn Bunpote, Pakkakul Sangsuriya, Saengchan Senapin, Prapansak Srisapoome, Pongsak Khunrae, Sasimanas Unajak, Ha Thanh Dong, Triwit Rattanarojpong
Summary: The study identified OmpA through immunoproteomic methods as a potential candidate for developing a recombinant vaccine against E. ictaluri infection in Nile tilapia. Immunization with recombinant OmpA protein induced specific antibody production and exhibited bacterial agglutination properties in fish serum. Partial protection was observed in Nile tilapia immunized with OmpA vaccine.
AQUACULTURE RESEARCH
(2022)
Article
Fisheries
Nguyen Vu Linh, Le Thanh Dien, Pattiya Sangpo, Saengchan Senapin, Anat Thapinta, Wattana Panphut, Sophie St-Hilaire, Channarong Rodkhum, Ha Thanh Dong
Summary: Nanobubble technology has potential applications in aquaculture and can enhance immune responses in fish. Pre-treatment with ozone nanobubbles improves the efficacy of immersion vaccination against Streptococcus agalactiae in Nile tilapia.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Ha Thanh Dong, Pattiya Sangpo, Le Thanh Dien, Thao Thu Mai, Nguyen Vu Linh, Jorge del-Pozo, Krishna R. Salin, Saengchan Senapin
Summary: CAIB in the pancreas of adult Nile tilapia infected with TiPV serves as a diagnostic feature, indicating exclusive replication of TiPV in pancreatic tissues, often accompanied by co-infection with Streptococcus agalactiae and secondary granulomas.
JOURNAL OF FISH DISEASES
(2022)
Article
Parasitology
Truong Dinh Hoai, Doan Thi Nhinh, Nguyen Thi Huong Giang, Saengchan Senapin, Ha Thanh Dong
Summary: This study reported the occurrence of Kudoa thunni infection in raw yellowfin tuna in Southeast Asia, raising public health concerns for the consumption of raw marine fish. The findings were confirmed through microscopic examination and nucleotide sequence analysis.
PARASITOLOGY INTERNATIONAL
(2022)
Article
Fisheries
Thitiya Rattanadilog Na Phuket, Walaiporn Charoensapsri, Piti Amparyup, Chanprapa Imjongjirak
Summary: The crab proline-rich AMP SpPR-AMP1 has potent antimicrobial activity against both Gram-positive and Gram-negative bacteria. This study demonstrates that SpPR-AMP1 has therapeutic effects against AHPND infection caused by Vibrio campbellii VH-639 and can enhance the innate immune response in shrimp Litopenaeus vannamei. SpPR-AMP1 disrupts the bacterial cell membrane and promotes shrimp's resistance against V. campbellii VH-639 infection.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Fisheries
Nguyen Dinh-Hung, Ha Thanh Dong, Suwimon Taengphu, Chayanit Soontara, Channarong Rodkhum, Saengchan Senapin, Satid Chatchaiphan
Summary: The objective of this study was to determine the causative agent of an outbreak with clinical signs similar to those of piscine streptococcosis in farmed snakeskin gourami (Trichopodus pectoralis). The bacterium isolated from the diseased fish was identified as Streptococcus suis, a new piscine pathogen. Experimental infection confirmed the pathogenicity of S. suis in both juvenile and adult fish, and histopathological changes were observed in multiple organs. Public awareness and biosecurity measures should be considered to prevent the spread of the disease.
Review
Fisheries
Olga L. M. Haenen, Ha Thanh Dong, Truong Dinh Hoai, Margaret Crumlish, Iddya Karunasagar, Timothy Barkham, Swaine L. Chen, Ruth Zadoks, Andreas Kiermeier, Bing Wang, Esther Garrido Gamarro, Masami Takeuchi, Mohammad Noor Amal Azmai, Belen Fouz, Rolando Pakingking, Zeng Wei Wei, Melba G. Bondad-Reantaso
Summary: Tilapia culture is a significant source of income and nutrition for rural families. However, intensification and global warming have led to bacterial disease outbreaks in tilapia farms, threatening sustainable production. This review provides an overview of common and emerging bacterial pathogens, diseases, diagnostics, and the risks of antimicrobial resistance in tilapia.
REVIEWS IN AQUACULTURE
(2023)
Review
Fisheries
Ha Thanh Dong, Thawatchai Chaijarasphong, Andrew C. Barnes, Jerome Delamare-Deboutteville, Peiyu Alison Lee, Saengchan Senapin, Chadag Vishnumurthy Mohan, Kathy F. J. Tang, Sharon E. McGladdery, Melba G. Bondad-Reantaso
Summary: The article "Diagnostics for diseases of tilapia: From basic to molecular diagnosis" explores the importance of the three levels of diagnostics for diseases of tilapia, particularly the frequently overlooked basic procedures. The article also covers current and emerging molecular diagnostic technologies for tilapia pathogens, and discusses future developments for transferring these technologies to farmers and stakeholders for a sustainable aquatic food system transformation.
REVIEWS IN AQUACULTURE
(2023)
Article
Fisheries
Nguyen Tien Vinh, Ha Thanh Dong, Nguyen Giang Thu Lan, Pakkakul Sangsuriya, Krishna R. Salin, Satid Chatchaiphan, Saengchan Senapin
Summary: In this study, the immune responses of Asian seabass fry at 35 and 42 days post-hatching to a heat-killed Streptococcus iniae vaccine were investigated. It was found that fish at both stages could produce specific antibodies against the pathogen, with the 42-day fry showing a faster response. These findings suggest that early vaccination of Asian seabass fry between 35 and 42 days post-hatching is feasible.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Fisheries
Sumit Vinod Jungi, Vimbai Irene Machimbirike, Nguyen Vu Linh, Pakkakul Sangsuriya, Krishna R. Salin, Saengchan Senapin, Ha Thanh Dong
Summary: This study explores the application of synthetic peptides as potential vaccine candidates for the prevention of NNV in Asian seabass. Three predicted immunogenic B cell epitopes were selected and synthesized, and the interaction between these peptides and Asian seabass's MHC class II was confirmed. The peptides exhibited both antigenic and immunogenic properties, suggesting their potential as vaccine candidates for preventing NNV infection in fish.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Fisheries
Ramida Phasunon, Suwimon Taengphu, Wattana Panphut, Satid Chatchaiphan, Ha Thanh Dong, Saengchan Senapin
Summary: A newly developed probe-based qPCR method for the detection of S. iniae was reported in this study. The method demonstrated high specificity and sensitivity, and can be applied for the diagnosis and surveillance of S. iniae infections in fish populations.
JOURNAL OF FISH DISEASES
(2023)
Article
Fisheries
Nguyen Vu Linh, Nareerat Khongcharoen, Dinh-Hung Nguyen, Le Thanh Dien, Naruporn Rungrueng, Chayuda Jhunkeaw, Pattiya Sangpo, Saengchan Senapin, Toungporn Uttarotai, Wattana Panphut, Sophie St-Hilaire, Hien Van Doan, Ha Thanh Dong
Summary: This study investigated the effects of oxygen nanobubble treatment on Nile tilapia. The results showed that both short-term and long-term exposure to high oxygen levels did not have obvious adverse effects on the fish, although there were some changes in the gut microbiome.
FISH & SHELLFISH IMMUNOLOGY
(2023)