Review
Plant Sciences
Wei Wang, Aifu Li, Zhihua Zhang, Chengcai Chu
Summary: The article provides an overview of posttranslational regulation of nitrogen uptake, assimilation, remobilization and signaling in plants, and how these regulations affect protein function and signal transduction. Understanding these mechanisms can help improve the nitrogen use efficiency of plants.
PLANT AND CELL PHYSIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Yangyang Gao, Shengdong Qi, Yong Wang
Summary: Understanding the molecular mechanisms of NO3- regulation in crops is crucial for improving nitrogen use efficiency (NUE) in agriculture. This article reviews recent advances in crop NO3- signaling, focusing on short-term signaling, long-term signaling, and the impact of environmental factors. The regulation of crop NUE by crucial genes involved in NO3- signaling is also discussed.
PLANT COMMUNICATIONS
(2022)
Article
Horticulture
Shuaiwei Zhang, Yuepeng Zhang, Yudan Wang, Yanwei Hao, Wei Su, Guangwen Sun, Houcheng Liu, Riyuan Chen, Shiwei Song
Summary: This study explored the sequences and expression characteristics of NRT genes in flowering Chinese cabbage and found that BcNRT1.1 and BcNRT1.2 were mainly expressed in roots and correlated with nitrate uptake rate. The expression of BcNRT1.2 was influenced by NH4+ concentration. Additionally, a mixed nutrition with an NH4+/NO3- of 25/75 significantly increased total nitrogen uptake in flowering Chinese cabbage.
Article
Multidisciplinary Sciences
Natalie M. Clark, Trevor M. Nolan, Ping Wang, Gaoyuan Song, Christian Montes, Conner T. Valentine, Hongqing Guo, Rosangela Sozzani, Yanhai Yin, Justin W. Walley
Summary: This study analyzed the BR signaling in Arabidopsis by integrating multiple omics datasets and inferring networks, identifying a BR-regulated transcription factor BRONTOSAURUS that affects cell division in roots. The research provides insights into the molecular signaling events during BR response through integrative network analysis applied to multi-omic data.
NATURE COMMUNICATIONS
(2021)
Article
Plant Sciences
Gaopeng Yuan, Dexi Sun, Yifan Wang, Guolin An, Weihua Li, Wenjing Si, Junpu Liu, Yingchun Zhu
Summary: This study comprehensively analyzed the NLP gene family in watermelon and found that ClNLP1 may play a crucial role in regulating the response of watermelon to nitrate nitrogen.
HORTICULTURAL PLANT JOURNAL
(2022)
Article
Biochemistry & Molecular Biology
Zhongtao Jia, Ricardo F. H. Giehl, Anja Hartmann, Jose M. Estevez, Malcolm J. Bennett, Nicolaus von Wiren
Summary: Root hairs play a crucial role in nutrient acquisition in plants. This study reveals the growth and regulatory mechanisms of root hairs in response to low nitrogen conditions. A cascade of molecular components involving auxin synthesis, transport, and signaling is activated, leading to elongation of root hairs for enhanced nutrient uptake in low nitrogen environments.
Article
Plant Sciences
Yangyang Gao, Shuxuan Quan, Bo Lyu, Tian Tian, Zhiguang Liu, Zhentian Nie, Shengdong Qi, Jingbo Jia, Jing Shu, Edwin Groot, Jiajie Wu, Yong Wang
Summary: The study investigated the role of barley transcription factor HvNLP2 in nitrate signaling and assimilation, revealing that HvNLP2 affects plant biomass, yield, and nitrogen use efficiency by controlling nitrate signaling.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Review
Biochemistry & Molecular Biology
Dawei Yan, Eiji Nambara
Summary: Nitrogen is essential for life, and plants have evolved various strategies to acquire it from the environment. Nodule Inception (NIN) and NIN-like protein (NLP) play critical roles in regulating nitrogen fixation and sensing nitrate levels in plants. This review summarizes recent progress in the study of NIN/NLP and their molecular basis in nitrate sensing and signaling.
Article
Agronomy
Jiapeng Xing, Yubin Wang, Qingqing Yao, Yushi Zhang, Mingcai Zhang, Zhaohu Li
Summary: Brassinosteroids (BRs) play an important role in regulating the physiological response to nitrogen (N) supply in maize. Exogenous application of BRs increases the primary root length and plant biomass of maize seedlings. Mutant plants with deficient BR signaling show shorter primary roots and lower plant biomass under low and normal N conditions. BRs modulate N accumulation and transportation, and promote N uptake in maize.
Review
Cell Biology
Paramjit S. Tappia, Naranjan S. Dhalla
Summary: The activation of phospholipase C (PLC) plays a key role in the response of cardiomyocytes to hypertrophic agents. PLC activity leads to the generation of downstream signal transducers, which in turn promote the expression of fetal genes and cardiomyocyte growth. This study focuses on the regulation of PLC gene expression, particularly in the norepinephrine-alpha(1)-adrenoceptor signaling pathway. It suggests that targeting specific PLC isozymes could potentially mitigate cardiac hypertrophy.
Article
Multidisciplinary Sciences
Suyu Jiang, Marie-Francoise Jardinaud, Jinpeng Gao, Yann Pecrix, Jiangqi Wen, Kirankumar Mysore, Ping Xu, Carmen Sanchez-Canizares, Yiting Ruan, Qiujiu Li, Meijun Zhu, Fuyu Li, Ertao Wang, Phillip S. Poole, Pascal Gamas, Jeremy D. Murray
Summary: Leghemoglobins play a crucial role in facilitating nitrogen fixation in legume nodules by maintaining a micro-oxic environment while channeling oxygen for bacterial respiration. The NLP transcription factors NLP2 and NIN are found to directly activate leghemoglobin expression through a specific promoter motif, and knockout of the NRE-like element significantly decreases leghemoglobin expression. These findings suggest that the NLP-leghemoglobin module for oxygen buffering in nodules has ancient origins dating back to the pairing of NLPs with nonsymbiotic hemoglobins in hypoxic conditions.
Article
Plant Sciences
Maria E. Sanchez-Sandoval, Graciela E. Racagni Di-Palma, Victor M. Gonzalez-Mendoza, Yahaira A. Cab-Guillen, Jose A. Munoz-Sanchez, Ana Ramos-Diaz, S. M. Teresa Hernandez-Sotomayor
Summary: Morphological changes in Capsicum plants were observed 24 hours after inoculation with a microbial consortium, mainly consisting of C. ignotum. High levels of diacylglycerol pyrophosphate (DGPP) and phosphatidic acid (PA) were found around 6 hours after inoculation, correlating with high transcription levels of diacylglycerol-kinase (CchDGK1 and CchDG31) and pathogen gene markers (CchPR1 and CchPR5).
Article
Environmental Sciences
Judit Kalman, Ana-Belen Muniz-Gonzalez, Maria-Angeles Garcia, Jose -Luis Martinez-Guitarte
Summary: Microplastics are a major concern for the environment, being found globally due to industrial production and plastic debris degradation. They are small in size, less than 5 mm, and can exist in soil and aquatic ecosystems at micro and nanometer scales. They can have diverse chemical compositions, including potentially toxic additives. The research shows that Chironomus riparius, an aquatic dipteran species, is able to ingest microplastics and it affects the metabolism of these organisms. However, the understanding of the molecular-level effects of microplastics on invertebrates is still limited.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Biotechnology & Applied Microbiology
Satoshi Yamamoto, Kouki Ito, Masaharu Tokuda, Naoaki Tsutsui
Summary: This study identified the RhNrt2 and RhCpeb genes and characterized their expression in Rhodomonas species. It was found that the expression level of RhNrt2 significantly increased under nitrogen-starved conditions, while the expression level of RhCpeb was significantly suppressed. The regulated expression of these genes may serve as molecular indicators of nitrate status and intracellular Phycoerythrin biosynthesis.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2023)
Article
Plant Sciences
Xinhui Zhang, Zhicai Xie, Duoyong Lang, Yuankui Chu, Gaochang Cui, Xiaoxia Jia
Summary: The study indicates that Bacillus pumilus G5 can enhance the growth of Glycyrrhiza uralensis seedlings under drought stress by regulating C and N metabolisms, leading to increased accumulation of secondary metabolites and ultimately improving drought tolerance.
PHYSIOLOGIA PLANTARUM
(2021)
Article
Biotechnology & Applied Microbiology
Alex Di Genova, Gino Nardocci, Rodrigo Maldonado-Agurto, Christian Hodar, Camilo Valdivieso, Pamela Morales, Felipe Gajardo, Raquel Marina, Rodrigo A. Gutierrez, Ariel Orellana, Veronica Cambiazo, Mauricio Gonzalez, Alvaro Glavic, Marco A. Mendez, Alejandro Maass, Miguel L. Allende, Martin A. Montecino
Summary: The study on Orestias ascotanensis from the Chilean Altiplano reveals a genomic evolution process involving gene contraction and expansion, particularly in genes related to genome stability and DNA repair under positive selection to cope with high-altitude aquatic environments.
Review
Biochemistry & Molecular Biology
Liliana Lamig, Sebastian Moreno, Jose M. Alvarez, Rodrigo A. Gutierrez
Summary: This minireview discusses the molecular mechanisms of how plants adapt to nitrate conditions and emphasizes the relationship between components of the nitrate signaling pathway and plant metabolic and developmental responses. Further research is needed to identify missing components of the pathway and validate their molecular interactions to explain the complexity of phenotypical responses to nitrate.
Article
Plant Sciences
Thomas Dussarrat, Sylvain Prigent, Claudio Latorre, Stephane Bernillon, Amelie Flandin, Francisca P. Diaz, Cedric Cassan, Pierre Van Delft, Daniel Jacob, Kranthi Varala, Jerome Joubes, Yves Gibon, Dominique Rolin, Rodrigo A. Gutierrez, Pierre Petriacq
Summary: By studying the metabolome of 24 plant species in the Atacama Desert, it was found that these plants adapt to extreme environments through common metabolites, which can predict the plant's environment. These findings are of great significance for improving crop yields under harsh conditions.
Article
Plant Sciences
Jonathan E. Maldonado, Alexis Gaete, Dinka Mandakovic, Constanza Aguado-Norese, Melissa Aguilar, Rodrigo A. Gutierrez, Mauricio Gonzalez
Summary: The discovery and characterization of plant species adapted to extreme environmental conditions, such as Hoffmannseggia doellii in the Chilean Atacama Desert, have become important. This study found that H. doellii has a higher microbial diversity in its root soil, suggesting a microbial diversity soil modulation mechanism that may be a characteristic of highly tolerant plants to diverse and extreme environments. Furthermore, the study provides an opportunity for future research on the climate change adaptation of crop plants.
Article
Plant Sciences
Javier Martinez Pacheco, Limei Song, Lenka Kubenova, Miroslav Ovecka, Victoria Berdion Gabarain, Juan Manuel Peralta, Tomas Urzua Lehuede, Miguel Angel Ibeas, Martiniano M. Ricardi, Sirui Zhu, Yanan Shen, Mikhail Schepetilnikov, Lyubov A. Ryabova, Jose M. Alvarez, Rodrigo A. Gutierrez, Guido Grossmann, Jozef Samaj, Feng Yu, Jose M. Estevez
Summary: This study investigated the roles of the cell surface receptor kinase FERONIA (FER) and the nutrient sensing TOR Complex 1 (TORC) in root hair (RH) growth using genetic approaches. It was found that low temperature triggers a strong RH elongation response involving FER and TORC in Arabidopsis thaliana. FER is required for perceiving limited nutrient availability caused by low temperature and interacts with and activates TORC-downstream components to trigger RH growth.
Article
Biology
Nathan R. Johnson, Luis F. Larrondo, Jose M. Alvarez, Elena A. Vidal
Summary: This study systematically assesses the characteristics and annotation reliability of mi/milRNA in fungi. It finds that less than half of the reported loci meet the basic standards for hairpin RNA discovery. Fungal mi/milRNAs are generally similar in size to animal miRNAs and are frequently associated with protein-coding genes. The study also identifies 25 conserved mi/milRNA loci across multiple species.
Article
Microbiology
Gabriel Perez-Lara, Tomas C. Moyano, Andrea Vega, Luis F. Larrondo, Ruben Polanco, Jose M. Alvarez, Daniel Aguayo, Paulo Canessa
Summary: To comprehensively analyze gene expression in the phytopathogenic fungus Botrytis cinerea, a web-based gene expression browser called BEB was developed. This computationally inexpensive application provides publication-ready heatmaps of transcript levels and allows gene expression clustering and visualization options, along with the ability to download gene expression values for further exploration. The implementation of BEB is transferable to other organisms and allows for comparative transcriptomics and candidate gene identification.
Article
Plant Sciences
Valentin Chaput, Jianfu Li, David Sere, Pascal Tillard, Cecile Fizames, Tomas Moyano, Kaijing Zuo, Antoine Martin, Rodrigo A. Gutierrez, Alain Gojon, Laurence Lejay
Summary: In this study, the researchers characterized and identified molecular elements involved in the repression of NRT2 transporters and root nitrate uptake in response to nitrate in Arabidopsis thaliana. They found that NRT2.4 and NRT2.5 are repressed by NRT1.1-mediated nitrate sensing. These findings provide important insights into the regulation of high-affinity nitrate uptake in Arabidopsis.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Genetics & Heredity
Dinka Mandakovic, Constanza Aguado-Norese, Beatriz Garcia-Jimenez, Christian Hodar, Jonathan E. Maldonado, Alexis Gaete, Mauricio Latorre, Mark D. Wilkinson, Rodrigo A. Gutierrez, Lohengrin A. Cavieres, Joaquin Medina, Veronica Cambiazo, Mauricio Gonzalez
Summary: This study investigated the interactions between soil microorganisms and native plants in the extreme environment of the Atacama Desert. The results showed that different plant species have specific effects on the composition and ecological interactions of soil bacterial communities. The study also identified the key taxa driving the transition of soil bacteria from bulk soil to the rhizosphere and found that the potential functions of bacterial communities vary between different vegetation belts. Overall, the findings highlight the importance of plant-microbe interactions in shaping the soil ecosystem in extreme environments.
ENVIRONMENTAL MICROBIOME
(2023)
Article
Biology
Sanae Kobayashi, Jonathan Maldonado, Alexis Gaete, Ingrid Araya, Constanza Aguado-Norese, Nicolas Cumplido, Sebastian Diaz, Alonso Espinoza, Edelmira Fernandez, Felipe Gajardo, Felipe Gonzalez-Ordenes, Khantati Hauyon, Piedad Maldonado, Rodrigo Maldonado, Isabel Pochet, Anibal Riveros, Paula Sandoval, Ailynne Sepulveda-Gonzalez, Camila Stuardo, Patricio Tapia-Reyes, Carolina Thornton, Soledad Undurraga, Macarena A. Varas, Camilo Valdivieso, Rodrigo A. Gutierrez, Ariel Orellana, Martin L. Montecino, Alejandro Maass, Mauricio Gonzalez, Miguel Allende, Christian Hodar, Paula Irles
Summary: Despite being the largest fraction of animal life, there are only a few hundred insect species whose genomes have been sequenced. The earwigs, as one of the basally derived insect groups and with its importance in agroecosystems, have lacked genomic information. However, a project was successfully carried out by engaging high school students from twelve schools in Chile to collect earwig specimens, extract genomic DNA, and perform genome sequencing using nanopore sequencers.
BIOLOGICAL RESEARCH
(2023)
Review
Plant Sciences
Ariel Cerda, Jose M. Alvarez
Summary: Drought and nitrate availability have significant impacts on plant growth and crop productivity. Understanding the regulatory mechanisms involved in the crosstalk between drought and nitrate signaling pathways can help enhance plant development and stress resistance, which are crucial for optimizing crop yield and promoting sustainable agriculture.
Article
Multidisciplinary Sciences
Orlando Contreras-Lopez, Elena A. Vidal, Eleodoro Riveras, Jose M. Alvarez, Tomas C. Moyano, Erin E. Sparks, Joaquin Medina, Angelo Pasquino, Philip N. Benfey, Gloria M. Coruzzi, Rodrigo A. Gutierrez
Summary: Nitrate, a nutrient and signaling molecule, affects global gene expression in plants. This study investigated the temporal and cell type-specific responses to nitrate in major root cell types of Arabidopsis thaliana. The gene expression response to nitrate was found to be dynamic and highly localized, and specific transcription factor (TF)-target interactions were predicted. The endodermis cell layer exhibited the largest and most connected nitrate-regulatory gene network, with ABF2 and ABF3 identified as major hubs for transcriptional responses. Experimental validation confirmed TF-target interactions for ABF2 and ABF3, which accounted for over 50% of the nitrate-responsive transcriptome in the endodermis. Additionally, ABF2 and ABF3 were found to play a role in nitrate-induced lateral root growth. This study provides unprecedented spatiotemporal resolution of root response to nitrate and identifies important components of cell-specific gene regulatory networks.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
