Article
Microbiology
Beate Schwer, Angad Garg, Ana M. Sanchez, Mindy A. Bernstein, Bradley Benjamin, Stewart Shuman
Summary: Inositol pyrophosphate dynamics regulate the phosphate homeostasis regulon in fission yeast through lncRNA-mediated transcription interference. A genetic screen identified several proteins that are involved in IPP toxicosis and shed light on its determinants.
Article
Biochemistry & Molecular Biology
Brian S. Mantilla, Karunakaran Kalesh, Nathaniel W. Brown, Dorothea Fiedler, Roberto Docampo
Summary: This study identified potential targets of 5-IP7 in Trypanosoma cruzi using pull-down experiments and mass spectrometry analysis, revealing new functional roles of 5-IP7 in this divergent eukaryote and providing potential new targets for chemotherapy.
MOLECULAR MICROBIOLOGY
(2021)
Article
Microbiology
Beate Schwer, Angad Garg, Ana M. Sanchez, Mindy A. Bernstein, Bradley Benjamin, Stewart Shuman
Summary: This study explored the role of IPP in regulating phosphate homeostasis in fission yeast and uncovered several proteins required for IPP toxicity, including Gde1, Spx1, and Vtc2/4, which possess specific enzymatic functions. The research revealed the involvement of Spx1 in transmitting the IP8 signal to the RNA processing machinery.
Article
Plant Sciences
Yonggang Zhou, Monan Sun, Pengyu Sun, Hongtao Gao, He Yang, Yan Jing, Rachit K. Saxena, Qingyu Wang, Haiyan Li, Muhammad Azhar Hussain, Foka Idrice Carther
Summary: This review summarizes the recent findings on the role of inositol transporters (INT) in plant metabolism and stress response mechanisms. It highlights the emerging role of vacuoles and vacuolar INT in plant growth and development. A comprehensive understanding of the INT functioning system will greatly advance the study of plant stress adaptation.
JOURNAL OF PLANT PHYSIOLOGY
(2022)
Article
Plant Sciences
Runze Guo, Qi Zhang, Yinghui Ying, Wenying Liao, Yu Liu, James Whelan, Mao Chuanzao, Huixia Shou
Summary: This study found that SPX-MFS genes in rice vacuoles are Pi influx transporters, with OsSPX-MFS3 playing the most important role in growth, vacuolar, and tissue Pi concentrations. Alteration of vacuolar Pi sequestration may improve rice tolerance to low phosphorus.
PLANT CELL AND ENVIRONMENT
(2023)
Article
Biochemistry & Molecular Biology
Noelia Lander, Miguel A. Chiurillo, Roberto Docampo
Summary: Trypanosoma cruzi is a unicellular parasite responsible for Chagas disease, with a digenetic life cycle alternating between mammalian and insect hosts. This parasite must adapt to various environmental conditions such as temperature, nutrient availability, pH, and oxidative stress, which are regulated by signaling pathways. This review will focus on the signaling pathways and second messengers involved in the responses to these environmental changes.
MOLECULAR MICROBIOLOGY
(2021)
Review
Agronomy
Tao Wu, Chuang Wang, Bei Han, Zhu Liu, Xinyu Yang, Wei Wang, Guangda Ding, John P. Hammond, Philip J. White, Fangsen Xu, Lei Shi
Summary: Phosphorus (P) is essential for plant growth and development, and inositol pyrophosphates (PP-InsPs) serve as important nutrient messengers in signaling P status. This review highlights the biosynthesis of PP-InsPs and their regulation by plant P status, the impact of PP-InsPs on SPX domain-containing proteins in P status signaling, the effects of inositol pyrophosphates on auxin and jasmonate signaling, and the potential crosstalk between P status signaling and phytohormone signaling mediated by inositol pyrophosphates. The interaction between inositol pyrophosphates and their binding proteins is crucial for plant P status and developmental responses to different P supply.
Article
Plant Sciences
Zhengrui Wang, Hui-Fen Kuo, Tzyy-Jen Chiou
Summary: Recent research has shown that inositol pyrophosphates act as phosphate signaling molecules, perceived by the SPX domains in eukaryotic cells to regulate Pi transport and signaling. The PP-InsP-SPX signaling module is evolutionarily conserved and extensively utilized in plant systems for Pi transport and signaling.
Article
Plant Sciences
Xianan Xie, Wenzhen Lai, Xianrong Che, Sijia Wang, Ying Ren, Wentao Hu, Hui Chen, Ming Tang
Summary: More than 70% of terrestrial vascular plants have a reciprocal symbiotic relationship with arbuscular mycorrhizal (AM) fungi, where plants provide fatty acids and sugars, and AM fungi facilitate plant phosphate (Pi) uptake. This study identifies a phosphate transporter called RiPT7 from Rhizophagus irregularis, which plays a crucial role in fine-tuning Pi homeostasis across the fungal membrane to maintain AM development.
Article
Biotechnology & Applied Microbiology
M. Plouviez, C. S. Oliveira da Rocha, B. Guieysse
Summary: The study investigated the changes in cellular phosphorus content and granular polyP abundance in Chlamydomonas reinhardtii under different phosphorus supply conditions. The results suggest that polyP functions as a reserve of phosphorus in the absence of readily available extracellular phosphorus and is not utilized as an energy source in darkness.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2022)
Article
Biochemistry & Molecular Biology
Elena Lobakova, Olga Gorelova, Irina Selyakh, Larisa Semenova, Pavel Scherbakov, Svetlana Vasilieva, Petr Zaytsev, Karina Shibzukhova, Olga Chivkunova, Olga Baulina, Alexei Solovchenko
Summary: This study reports an exception of high phosphate resilience in Microactinium simplicissimum IPPAS C-2056, a microalgal species that cannot adapt to high concentrations of phosphate after a period of phosphate starvation. The failure of resilience may be attributed to the rapid formation of potentially toxic short-chain polyphosphate following the influx of phosphate into the starved cells. The findings have implications for avoiding sudden culture crashes and developing efficient algae-based technologies for phosphorus removal from waste streams.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Boah Lee, Seung Ju Park, Sehoon Hong, Kyunghan Kim, Seyun Kim
Summary: IPMK is a promiscuous enzyme with broad substrate specificity, phosphorylating multiple inositol phosphates and phosphatidylinositol 4,5-bisphosphate. In addition to its catalytic actions, IPMK can control signaling events via direct protein-protein interactions. It plays pleiotropic roles in physiological and pathological conditions.
MOLECULES AND CELLS
(2021)
Article
Biochemistry & Molecular Biology
Long Wang, Xianqing Jia, Yuxin Zhang, Lei Xu, Benoit Menand, Hongyu Zhao, Houqing Zeng, Liam Dolan, Yiyong Zhu, Keke Yi
Summary: The study revealed the crucial roles of CrPTC1 and CrVTC4 genes in polyP accumulation in vacuoles of chlorophyte algae. Phylogenetic analysis showed the conservation of certain SPX proteins in the common ancestor of green plants, with differential expression patterns in various plant types.
Article
Multidisciplinary Sciences
Hyungyu Min, Wooseob Kim, Sehoon Hong, Sungkyu Lee, Jinguk Jeong, Seyun Kim, Rho H. Seong
Summary: This study reveals that inositol polyphosphate multikinase (IPMK) is a crucial regulator of Treg cell differentiation downstream of TCR signaling, and deficiency of IPMK leads to abnormal T cell activation and impaired Treg cell differentiation and function.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Environmental Sciences
Xiang-Yu Long, Ran Tang, Tao Wang, Guang-Jian Tao, Jia-Yue Wang, Hai-Wei Zhou, Ming Xue, Yong-Ping Yu
Summary: The study investigated the characteristics of enhanced biological phosphorus removal process under the combined actions of intracellular and extracellular polyphosphate using P-31 Nuclear Magnetic Resonance and fractionation methods. It found that the hydrolysis/synthesis of polyP played a key role in phosphate migration and transformation, leading to the absorption and transformation of VFAs, as well as the continuous hydrolysis and synthesis of PHAs. The accumulation of orthoP in different compartments drove the migration of orthoP and VFAs, promoting the overall efficiency of phosphorus removal in EBPR.
Article
Plant Sciences
Shu-Yi Yang, Wen-Chien Lu, Swee-Suak Ko, Ching-Mei Sun, Jo-Chi Hung, Tzyy-Jen Chiou
Article
Plant Sciences
Abira Sahu, Swayoma Banerjee, Aditi Subramani Raju, Tzyy-Jen Chiou, L. Rene Garcia, Wayne K. Versaw
Review
Plant Sciences
Ching Chan, Ya-Yun Liao, Tzyy-Jen Chiou
Summary: Phosphorus is essential for plant growth and plays a significant role in plant immunity. Recent studies have shown that external soil P availability, internal cellular P levels, P starvation signaling machinery, and phosphate transporters are crucial for biotic interactions. Additionally, plant hormones are found to impact the signaling convergence between cellular P and immune responses.
PLANT AND CELL PHYSIOLOGY
(2021)
Editorial Material
Plant Sciences
Tzyy-Jen Chiou
PLANT AND CELL PHYSIOLOGY
(2021)
Article
Plant Sciences
Beatriz Val-Torregrosa, Mireia Bundo, Hector Martin-Cardoso, Marcel Bach-Pages, Tzyy-Jen Chiou, Victor Flors, Blanca San Segundo
Summary: In Arabidopsis, high phosphate treatment increases Pi content and reactive oxygen species (ROS) accumulation, resulting in resistance to fungal pathogen infection. High phosphate treatment also upregulates the expression of genes in the salicylic acid (SA) and jasmonic acid (JA) signaling pathways in non-infected plants. This study suggests that phosphate accumulation promotes immune responses in Arabidopsis and provides insights into the interactions between Pi signaling and hormonal signaling pathways.
Article
Plant Sciences
Yu-Heng Hsieh, Yi-Hsien Wei, Jui-Chi Lo, Hsuan-Yu Pan, Shu-Yi Yang
Summary: Plant lateral root growth is regulated by arbuscular mycorrhizal symbiosis, with CEP2 playing a critical role in modulating this process through the auxin-related pathway.
Article
Plant Sciences
Pei-Shan Chien, Ya-Ting Chao, Chia-Hui Chou, Yu-Ying Hsu, Su-Fen Chiang, Chih-Wei Tung, Tzyy-Jen Chiou
Summary: This study provides insights into the genetic basis of phosphorus acquisition regulation and the relationship between phosphate transporter PHT1;1 haplotype and Arabidopsis habitats.
Article
Biochemistry & Molecular Biology
Beatriz Val-Torregrosa, Mireia Bundo, Mani Deepika Mallavarapu, Tzyy-Jen Chiou, Victor Flors, Blanca San Segundo
Summary: This study reveals the important role of NLA gene in Arabidopsis immunity and its connection to phosphorus signaling. Loss-of-function of NLA and overexpression of MIR827 enhance phosphorus uptake and resistance to fungal infection in plants.
Article
Plant Sciences
Chen Hsieh, Yun-Hsin Chen, Kai-Chieh Chang, Shu-Yi Yang
Summary: More than half of the global population relies on rice as a staple food. This study reveals that arbuscular mycorrhizal (AM) symbiosis improves the growth and salt tolerance of rice by influencing gene expression and enhancing reactive oxygen species scavenging capacity.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Hayley L. Whitfield, Sining He, Yinghong Gu, Colleen Sprigg, Hui -Fen Kuo, Tzyy-Jen Chiou, Andrew M. Riley, Barry V. L. Potter, Andrew M. Hemmings, Charles A. Brearley
Summary: This study describes the differences in substrate specificity between two isoforms of ITPK enzymes (ITPK1 and ITPK4) in Arabidopsis, and provides a molecular explanation for the diverse phosphotransferase activity of ITPK4 by analyzing its ATP-coordinated structure. Furthermore, the study reveals a previously unreported N-terminal haloacid dehalogenase-like fold in ITPK4 and its homologues in other plants.
BIOCHEMICAL JOURNAL
(2023)
Article
Plant Sciences
Chang-Yi Chiu, Hui-Fang Lung, Wen-Chun Chou, Li-Yen Lin, Hong-Xuan Chow, Yu-Hao Kuo, Pei-Shan Chien, Tzyy-Jen Chiou, Tzu-Yin Liu
Summary: Autophagy in plants is regulated by different signaling cascades in response to environmental changes. Fine-tuning of its activity is critical for cellular homeostasis. This study observed that Pi deficiency moderately upregulates most ATG genes and increases autophagic structures in the root of Arabidopsis, but does not significantly alter autophagic flux in the whole roots. At the organismal level, mutants of ATG exhibited decreased shoot Pi concentrations and smaller meristem sizes under Pi sufficiency.
PLANT AND CELL PHYSIOLOGY
(2023)
Article
Plant Sciences
Joseph Yayen, Ching Chan, Ching-Mei Sun, Su-Fen Chiang, Tzyy-Jen Chiou
Summary: The number of genes encoding receptor-like kinases (RLKs) has expanded in land plants, resulting in diverse domain architectures. This study focused on RLCK XI, a subfamily of receptor-like cytoplasmic kinases in plants. The researchers discovered a long kinase insert domain (KID) between subdomains VII and VIII in RLCK XI, with an average length of 280 amino acids. This unique KID architecture was found in several hornwort and liverwort species. The KID showed a high propensity for being disordered and contained repetitive nuclear localization signals, suggesting a dual localization of RLCK XI from the plasma membrane to the nucleus.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Plant Sciences
Pei-Shan Chien, Pin-Hua Chen, Cheng-Ruei Lee, Tzyy-Jen Chiou
Summary: Genome-wide association studies (GWAS) have improved our understanding of complex traits, but challenges remain in distinguishing causation from linkage disequilibrium-caused association. Transcriptome-wide association studies (TWAS) offer an opportunity to prioritize candidate genes by identifying direct associations between expression levels and phenotypic variations. In this study, the feasibility of TWAS was assessed in Arabidopsis, specifically regarding flowering time. TWAS analysis identified several associated genes known to regulate growth allometry or metabolite production, and functionally validated six newly identified genes for flowering time.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Plant Sciences
Chih-Pin Chiang, Jia-Ling Li, Tzyy-Jen Chiou
Summary: MicroRNA399 (miR399), induced by phosphate starvation, is produced in shoots and moves to roots to suppress the gene PHO2, enhancing phosphate uptake and translocation from roots to shoots.
Article
Plant Sciences
Zhengrui Wang, Hui-Fen Kuo, Tzyy-Jen Chiou
Summary: Recent research has shown that inositol pyrophosphates act as phosphate signaling molecules, perceived by the SPX domains in eukaryotic cells to regulate Pi transport and signaling. The PP-InsP-SPX signaling module is evolutionarily conserved and extensively utilized in plant systems for Pi transport and signaling.